dehydrative S‐allylation

High-throughput DNA synthesis for data storage

“…Temperature is a key factor in controlling the start or end of the reaction, and it also influences the quality of products. 32,137,[148][149][150] Evonetix is the representative company for heat-controlled chip-based DNA synthesis technology. Andrew J. Ferguson et al managed to control the synthesis process by precisely adjusting the temperature of reaction sites, combined with a microfluidic system.…”

“…Temperature is a key factor in controlling the start or end of the reaction, and it also influences the quality of products. 32,137,148–150…”

“…1): encoding, writing, preservation, reading, and decoding. 8,12,18,[28][29][30][31][32][33][34][35] This journal is © The Royal Society of Chemistry 2024 Specifically, ''encoding'' refers to the conversion of the binary digital data (0/1) of the initial data into DNA base sequences (A/G/C/T) arranged in specific order according to the corresponding coding algorithms, such as: Huffman-code, 14,46 Fountain code, 23 Reed Solomon (RS) code, 15,45 and yin-yang code (YYC). 47 Usually, the final base sequences don't only contain valid data fragments, but may also include assisting fragments such as (1) primers located at both ends of a strand.…”

“…These technologies are based on the four-step phosphoramidite chemistry but with different deprotection mechanisms. These are realized by involving terminal protecting groups of different natures in the synthesis process, such as pH-sensitive protecting groups, 80,116,[132][133][134][135] temperature-sensitive protecting groups 32,136,137 or photolabile groups. [138][139][140] Several array-based DNA synthesis methods are briefly illustrated in Fig.…”

“…In the field of DNA data storage, there are already a large number of previous works on topics such as coding/decoding algorithms, 14,17,[22][23][24] error correction mechanisms, 15,20,25 preservation methods, 19,26,27 and overall reviews. 12,18,[28][29][30][31][32][33][34][35] Complementary to these articles and reviews, here, our discussion mainly focuses on the field of hardware manufacturing that is committed to the use of silicon-based chip technology to develop micro and nano scale integrated chips for highthroughput artificial DNA synthesis platforms: The history and current situation of DNA synthesis technology are introduced. Several high-throughput array-based DNA synthesis technologies are described in detail, and the state-of-the-art representative companies are listed.…”