Dehydrated human amniotic membrane modulates canonical Wnt signaling in multiple cell types in vitro

Moreno, Sarah; Massee, Michelle; Bara, Heather; Koob, Thomas J.

doi:10.1016/j.ejcb.2021.151168

Cited by 6 publications

(4 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In SFiPSCs and PBiPSCs, marker genes for skin fibroblasts (CDK1 and NOTCH3) and those for peripheral blood (STMN3) were highly expressed in the cells that originated from the corresponding tissues, respectively. On the other hand, in AMiPSCs, positive expression of a marker gene for amniotic membrane (POSTN) was not observed unlike previously reported ( 75 ), although negative expressions of AXIN2 and TCF7 were consistent with previous findings ( 76 , 77 ). Apparently, two subsets of ESCs belonging to C1 and C2, respectively, showed a similar expression pattern to those of iPSCs in each cluster.…”

Section: Resultssupporting

confidence: 89%

Comprehensive comparison of gene expression diversity among a variety of human stem cells

Yamatani

Nakai

2022

NAR Genomics and Bioinformatics

View full text Add to dashboard Cite

Several factors, including tissue origins and culture conditions, affect the gene expression of undifferentiated stem cells. However, understanding the basic identity across different stem cells has not been pursued well despite its importance in stem cell biology. Thus, we aimed to rank the relative importance of multiple factors to gene expression profile among undifferentiated human stem cells by analyzing publicly available RNA-seq datasets. We first conducted batch effect correction to avoid undefined variance in the dataset as possible. Then, we highlighted the relative impact of biological and technical factors among undifferentiated stem cell types: a more influence on tissue origins in induced pluripotent stem cells than in other stem cell types; a stronger impact of culture condition in embryonic stem cells and somatic stem cell types, including mesenchymal stem cells and hematopoietic stem cells. In addition, we found that a characteristic gene module, enriched in histones, exhibits higher expression across different stem cell types that were annotated by specific culture conditions. This tendency was also observed in mouse stem cell RNA-seq data. Our findings would help to obtain general insights into stem cell quality, such as the balance of differentiation potentials that undifferentiated stem cells possess.

show abstract

Section: Resultssupporting

confidence: 89%

Comprehensive comparison of gene expression diversity among a variety of human stem cells

Yamatani

Nakai

2022

NAR Genomics and Bioinformatics

View full text Add to dashboard Cite

show abstract

“…It is designed to monitor the transcriptional activity of TCF/LEF and can be used for studying Wnt signaling pathways, as well as screening of activators or inhibitors that affect the TCF/LEF transcriptional activity. 21 , 22 In this study, the cells were firstly co-transfected with a Wnt-1 plasmid and a sclerostin plasmid to construct a model in which Wnt signaling induced by Wnt-1 was greatly suppressed by sclerostin. After the cells were treated with sclerostin inhibitors, the Wnt signaling would be reactivated.…”

Section: Resultsmentioning

confidence: 99%

“…Referring to a published protocol, 21 , 22 the TCF/LEF luciferase Reporter HEK293 cells were cultured at 37°C under humidified atmosphere and 5% CO 2 with DMEM containing 10% FBS and 1% penicillin-streptomycin (Gibco by Life Technology). After seeded on 96-well plate at a density of 5 × 10 4 in 100 μL DMEM, the cells were cultured 24 h. A Wnt-1 plasmid (25 ng/well) and a sclerostin/sclerostin C124A plasmid (25 ng/well) were co-transfected into the cells using lipofectamine 2000 (0.35 μL per well).…”

Section: Methodsmentioning

confidence: 99%

Unique quinoline orientations shape the modified aptamer to sclerostin for enhanced binding affinity and bone anabolic potential

Gubu,

Ma,

et al. 2024

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

“…21,23,25,26,43,44 Through more advanced analysis, it has been demonstrated that amniotic membrane can not only impact cellular signaling but also that its effects can vary depending upon the environment in which it is placed. [43][44][45] Functional assays derived from the critical steps in the healing cascade were developed to model the role LHACM and DHACM may play in clinical applications. Fibroblast migration and proliferation are essential and rate-limiting steps to repair wounds due to their central role in the formation of granulation tissue which can provide a matrix for additional cells and blood vessels to grow.…”

Section: Discussionmentioning

confidence: 99%

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

Moreno,

Massee,

Campbell

et al. 2024

J Biomater Appl

Self Cite

View full text Add to dashboard Cite

The reparative properties of amniotic membrane allografts are well-suited for a broad spectrum of specialties. Further enhancement of their utility can be achieved by designing to the needs of each application through the development of novel processing techniques and tissue configurations. As such, this study evaluated the material characteristics and biological properties of two PURION® processed amniotic membrane products, a lyophilized human amnion, intermediate layer, and chorion membrane (LHACM) and a dehydrated human amnion, chorion membrane (DHACM). LHACM is thicker; therefore, its handling properties are ideal for deep, soft tissue deficits; whereas DHACM is more similar to a film-like overlay and may be used for shallow defects or surgical on-lays. Characterization of the similarities and differences between LHACM and DHACM was conducted through a series of in vitro and in vivo studies relevant to the healing cascade. Compositional analysis was performed through histological staining along with assessment of barrier membrane properties through equilibrium dialysis. In vitro cellular response was assessed in fibroblasts and endothelial cells using cell proliferation, migration, and metabolic assays. The in vivo cellular response was assessed in an athymic nude mouse subcutaneous implantation model. The results indicated the PURION® process preserved the native membrane structure, nonviable cells and collagen distributed in the individual layers of both products. Although, LHACM is thicker than DHACM, a similar composition of growth factors, cytokines, chemokines and proteases is retained and consequently elicit comparable in vitro and in vivo cellular responses. In culture, both treatments behaved as potent mitogens, chemoattractants and stimulants, which translated to the promotion of cellular infiltration, neocollagen deposition and angiogenesis in a murine model. PURION® processed LHACM and DHACM differ in physical properties but possess similar in vitro and in vivo activities highlighting the impact of processing method on the versatility of clinical use of amniotic membrane allografts.

show abstract

Dehydrated human amniotic membrane modulates canonical Wnt signaling in multiple cell types in vitro

Cited by 6 publications

References 70 publications

Comprehensive comparison of gene expression diversity among a variety of human stem cells

Comprehensive comparison of gene expression diversity among a variety of human stem cells

Unique quinoline orientations shape the modified aptamer to sclerostin for enhanced binding affinity and bone anabolic potential

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

Contact Info

Product

Resources

About

Dehydrated human amniotic membrane modulates canonical Wnt signaling in multiple cell types in vitro

Cited by 6 publications

References 70 publications

Comprehensive comparison of gene expression diversity among a variety of human stem cells

Comprehensive comparison of gene expression diversity among a variety of human stem cells

Unique quinoline orientations shape the modified aptamer to sclerostin for enhanced binding affinity and bone anabolic potential

PURION® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

Contact Info

Product

Resources

About

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair