Degradation products of the mRNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean and transgenic petunia.

Thompson, Deborah; Tanzer, Matthew M.; Meagher, Richard B.

doi:10.1105/tpc.4.1.47

Cited by 33 publications

(57 citation statements)

References 40 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This stabilization could occur because translation of a labile transacting factor, or translation of the mRNA itself, is required for rapid degradation. The role of polyribosomes (polysomes) as sites of mRNA decay is supported by reports that the decay intermediates of SRS4 (small subunit of ribulose-1,5-bisphosphate carboxylase in soybean [Glycine max]) and PHYA (phytochrome A in oat [Avena sativa]) mRNAs are polysome associated (Thompson et al, 1992;Higgs and Colbert, 1994) and that most in vitro decay systems are polysome based (Tanzer and Meagher, 1994;Ross, 1995). In another model system, several mutations of the pea Fed-1 gene that block translational initiation or elongation also abolish the light response of Fed-1, consistent with the model of translation being a requirement for the stabilization of Fed-1 in a light environment (Chiba and Green, 2009).…”

mentioning

confidence: 89%

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

et al. 2012

View full text Add to dashboard Cite

Abiotic stress, including drought, salinity, and temperature extremes, regulates gene expression at the transcriptional and posttranscriptional levels. Expression profiling of total messenger RNAs (mRNAs) from rice (Oryza sativa) leaves grown under stress conditions revealed that the transcript levels of photosynthetic genes are reduced more rapidly than others, a phenomenon referred to as stress-induced mRNA decay (SMD). By comparing RNA polymerase II engagement with the steady-state mRNA level, we show here that SMD is a posttranscriptional event. The SMD of photosynthetic genes was further verified by measuring the half-lives of the small subunit of Rubisco (RbcS1) and Chlorophyll a/b-Binding Protein1 (Cab1) mRNAs during stress conditions in the presence of the transcription inhibitor cordycepin. To discern any correlation between SMD and the process of translation, changes in total and polysome-associated mRNA levels after stress were measured. Total and polysome-associated mRNA levels of two photosynthetic (RbcS1 and Cab1) and two stress-inducible (Dehydration Stress-Inducible Protein1 and Salt-Induced Protein) genes were found to be markedly similar. This demonstrated the importance of polysome association for transcript stability under stress conditions. Microarray experiments performed on total and polysomal mRNAs indicate that approximately half of all mRNAs that undergo SMD remain polysome associated during stress treatments. To delineate the functional determinant(s) of mRNAs responsible for SMD, the RbcS1 and Cab1 transcripts were dissected into several components. The expressions of different combinations of the mRNA components were analyzed under stress conditions, revealing that both 39 and 59 untranslated regions are necessary for SMD. Our results, therefore, suggest that the posttranscriptional control of photosynthetic mRNA decay under stress conditions requires both 39 and 59 untranslated regions and correlates with differential polysome association.

show abstract

mentioning

confidence: 89%

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Poly(A) tail-shortening has been shown to precede the decay of several mammalian and yeast mRNAs (Decker and Parker, 1994;Muhlrad and Parker, 1992;Shyu et al, 1991). In plants, most of the degradative intermediates of the soybean SRS4 mRNAs (which encode the small subunit of ribulose bisphosphate carboxylase) lack poly(A) tails, suggesting that removal of poly(A) tails is an early step in the degradation of these mRNAs (Thompson et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Pollination induces mRNA poly(A) tail‐shortening and cell deterioration in flower transmitting tissue

Wang

Cheung³

1996

The Plant Journal

View full text Add to dashboard Cite

SummaryPollination induces many physiological responses in the flower, including deterioration and death in specific pistil cell types. It is shown here that within the style of tobacco, pollination-induced cell deterioration was restricted to the transmitting tissue while the surrounding cortical tissue was not affected. It was distinct from general senescence since exogenously applying the senescence-inducing hormone ethylene, or its precursor aminocyclopropane-1-carboxylic acid (ACC), to the flower or the pistil induced overall deterioration in the entire flower. Furthermore, both pollen tube growth and ethylene action were needed for the entire spectrum of cellular changes associated with this pollination-induced transmitting tissue deterioration process. It is also shown that pollination-induced mRNA poly(A) tail-shortening for at least three major classes of transmitting tissue-specific mRNAs. As is commonly observed for poly(A) tail-shortened mRNAs, the levels of two of these three mRNA classes decline after pollination. On the other hand, the third class of mRNAs, transmitting tissue-specific ('r'rs) mRNAs, was maintained at a very high level subsequent to pollination, even after substantial poly(A)-tail shortening. TTS mRNAs encode a pollen tube growth-promoting and -attracting protein needed for optimal in vivo pollen tube growth. The specific preservation of TTS mRNAs in the deteriorating transmitting tissue cells suggests that these cells can distinguish molecules needed in the pollinated styles from those that are dispensable, and protect them from degradation. It is suggested that the pollination-induced mRNA poly(A) tail-shortening and cell death are programmed processes suited to the post-pollination transmitting tissue environment. Results showing that ACC is a candidate signal molecule for the pollination-induced mRNA-shortening which is accentuated by ethylene and mediated via a protein phosphorylation-dependent signal transduction pathway are also presented.

show abstract

“…Procedures for RNA gel blot analysis and hybridization of total maize RNA from agarose gels were described by Seeley et al (1992). Procedures for polyacrylamide gel blot analyses of RNA were described by Thompson et al (1992).…”

Section: Maize Transcript Analysesmentioning

confidence: 99%

empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

Meeley

Scanlon

2002

Plant Cell

View full text Add to dashboard Cite

The heat shock response (HSR) is an evolutionarily conserved molecular/biochemical reaction to thermal stress that is essential to the survival of eukaryotic organisms. Recessive Mutator transposon mutations at the maize empty pericarp2 ( emp2 ) locus led to dramatically increased expression of heat shock genes, retarded embryo development, and early-stage abortion of embryogenesis. The developmental timing of emp2 mutant embryo lethality was correlated with the initial competence of maize kernels to invoke the HSR. Cloning and sequence analyses revealed that the emp2 gene encoded a predicted protein with high similarity to HEAT SHOCK BINDING PROTEIN1, which was first described in animals as a negative regulator of the HSR. emp2 is a loss-of-function mutation of an HSR-negative regulator in plants. Despite the recessive emp2 phenotype, steady state levels of emp2 transcripts were abundant in mutant kernels, and the predicted coding region was unaffected. These expression data suggest that emp2 transcription is feedback regulated, whereas S1 nuclease mapping suggests that emp2 mutant transcripts are 5 Ј truncated and nontranslatable. In support of this model, immunoblot assays revealed that EMP2 protein did not accumulate in mutant kernels. These data support a model whereby an unattenuated HSR results in the early abortion of emp2 mutant embryos. Furthermore, the developmental retardation of emp2 mutant kernels before the HSR suggests an additional role for EMP2 during embryo development distinct from the HSR.

show abstract

Degradation products of the mRNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean and transgenic petunia.

Cited by 33 publications

References 40 publications

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3′ and 5′ Untranslated Regions and Correlates with Differential Polysome Association in Rice

Pollination induces mRNA poly(A) tail‐shortening and cell deterioration in flower transmitting tissue

empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis

Contact Info

Product

Resources

About