Degradation potential of biological tissues fixed with various fixatives: Anin vitro study

Sung, Hsing‐Wen; Hsu, Chin-Sheng; Wang, Shengping; Hsu, Hung-Liang

doi:10.1002/(sici)1097-4636(199705)35:2<147::aid-jbm2>3.0.co;2-n

Cited by 38 publications

(16 citation statements)

References 28 publications

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“…Moreover, the introduction of cross-links by GA causes resistance to the rearrangement of collagen in the direction of the applied load. A similar study by Sung, et al , in porcine pericardia, where free amino group content and tensile tests were performed after FA and GA exposure for 3 days, reported significant reductions in free amino groups content as compared to fresh ones [54]. This suggests that FA and GA were effective cross-linking agents.…”

Section: Discussionmentioning

confidence: 76%

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Shearwave dispersion ultrasound vibrometry (sduv) on swine kidney

Amador

Urban

Chen

et al. 2011

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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Shearwave Dispersion Ultrasound Vibrometry (SDUV) is used to quantify both tissue shear elasticity and shear viscosity by evaluating dispersion of shear wave propagation speed over a certain bandwidth (50–500 Hz). The motivation for developing elasticity imaging techniques is based on the possibility of diagnosing disease process. However, it is important to study the mechanical properties of healthy tissues; such data can enhance clinical knowledge and improve understanding of the mechanical properties of tissue. The purpose of this study is to evaluate the feasibility of SDUV for in vitro measurements of renal cortex shear elasticity and shear viscosity on healthy swine kidney. A total of eight excised kidneys from female pigs were used in these in vitro experiments, and a battery of different tests were performed to gain insight on the material properties of the renal cortex. From these eight kidneys, the overall renal cortex elasticity and viscosity was 1.81 ± 0.17 kPa and 1.48 ± 0.49 Pa·s, respectively. In an analysis of the material properties over time after excision, there was not a statistically significant difference in shear elasticity over a 24 hour period, but a statistically significant difference in shear viscosity was found. Homogeneity of the renal cortex was examined and it was found that shear elasticity and shear viscosity were statistically different within a kidney, suggesting global tissue inhomogeneity. More than 30% increases in shear elasticity and shear viscosity were observed after immersion in 10% formaldehyde. Lastly, it was found that the renal cortex is rather anisotropic. Two values for shear elasticity and shear viscosity were measured depending on shear wave propagation direction. These various tests elucidated different aspects of the material properties and the structure of the ex vivo renal cortex.

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Section: Discussionmentioning

confidence: 76%

“…Tissue stiffness can be increased by inducing collagen cross-link with aldehydes [50-54]. Differences in the material properties in the renal cortex when exposed to a chemical agent that changed the tissue structure and stiffness were explored.…”

Section: Methodsmentioning

confidence: 99%

Shearwave dispersion ultrasound vibrometry (sduv) on swine kidney

Amador

Urban

Chen

et al. 2011

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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“…However, degradation in vitro using bacterial collagenase has been shown to correlate well with in vivo degradation, despite the differences in the cleavage mechanism [ 17 ]. Therefore, in vitro analysis can be used as a preliminary mechanism for determining degradative behavior [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility and degradation of tendon-derived scaffolds

Alberti

2015

Regen Biomater

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Decellularized extracellular matrix has often been used as a biomaterial for tissue engineering applications. Its function, once implanted can be crucial to determining whether a tissue engineered construct will be successful, both in terms of how the material breaks down, and how the body reacts to the material’s presence in the first place. Collagen is one of the primary components of extracellular matrix and has been used for a number of biomedical applications. Scaffolds comprised of highly aligned collagen fibrils can be fabricated directly from decellularized tendon using a slicing, stacking, and rolling technique, to create two- and three-dimensional constructs. Here, the degradation characteristics of the material are evaluated in vitro, showing that chemical crosslinking can reduce degradation while maintaining fiber structure. In vivo, non-crosslinked and crosslinked samples are implanted, and their biological response and degradation evaluated through histological sectioning, trichrome staining, and immunohistochemical staining for macrophages. Non-crosslinked samples are rapidly degraded and lose fiber morphology while crosslinked samples retain both macroscopic structure as well as fiber orientation. The cellular response of both materials is also investigated. The in vivo response demonstrates that the decellularized tendon material is biocompatible, biodegradable and can be crosslinked to maintain surface features for extended periods of time in vivo. This study provides material characteristics for the use of decellularized tendon as biomaterial for tissue engineering.

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“… 28 Moreover, a dense layered network structure in fixed BAM tissues was formed, which was an obstacle for enzymatic penetration into fixed-BAM tissues. 29 …”

Section: Resultsmentioning

confidence: 99%