Degradation of β-lactam antibiotic ampicillin using sustainable microbial peroxide producing cell system

Mukhopadhyay, Dhruva; Khan, Nawaz; Kamal, Neha; Varjani, Sunita; Singh, Sonal; Sindhu, Raveendran; Gupta, Pratima; Bhargava, Preeti Chaturvedi

doi:10.1016/j.biortech.2022.127605

Cited by 10 publications

(5 citation statements)

References 45 publications

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“…20 Mukhopadhyay et al explored the degradation of the beta-lactam antibiotic ampicillin utilizing a self-sustaining MPPC system with maximum electrical output in the range of 92−117 mV and a maximum H 2 O 2 production of 36−88 mM concentration,. 21 At an applied voltage of 200 mV, Dongwon Ki illustrated the H 2 O 2 production in an MPPC fed with primary sludge, accomplishing the total electrochemical production of H 2 O 2 of 230 mg/L. 22 In the present study, the concentration of the microbial electrochemically produced hydrogen peroxide in the catholyte of MPPC d and e was greater compared to the other catholyte, and this was accurate with their greatest electrochemical activity in terms of voltage generation.…”

Section: ■ Introductionsupporting

confidence: 58%

“…Utilizing heat-treated graphite electrodes, Asghar and colleagues studied the production of H 2 O 2 , achieving a maximum of 140 mg/L of H 2 O 2 with electrochemical voltage production in the range of 410–580 mV . Mukhopadhyay et al explored the degradation of the beta-lactam antibiotic ampicillin utilizing a self-sustaining MPPC system with maximum electrical output in the range of 92–117 mV and a maximum H 2 O 2 production of 36–88 mM concentration, . At an applied voltage of 200 mV, Dongwon Ki illustrated the H 2 O 2 production in an MPPC fed with primary sludge, accomplishing the total electrochemical production of H 2 O 2 of 230 mg/L …”

Section: Resultsmentioning

confidence: 99%

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Exploring the Oxidative Effects of the Microbial Electro-Fenton Process on the Depolymerization of Lignin Extracted from Rice Straw in a Bio-Electrochemical System Coupled with Wastewater Treatment

Chang

Gupta

2023

Biomacromolecules

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Lignin is a potential renewable feedstock to produce value-added compounds, but the overwhelming bulk of it is either burned for energy or discarded as waste. This paper addressed two critical issues: waste-to-value generation and management by demonstrating the in situ depolymerization of lignin extracted from waste rice straw utilizing the microbial electro-Fenton process in a microbial peroxide-producing cell (MPPC), a type of bio-electrochemical cell, for value addition while synchronously treating wastewater. The MPPC electrochemical voltage yields of 0.171 ± 0.05–0.497 ± 0.2 V produced 9 ± 0.43–34 ± 0.11 mM of H2O2, which was utilized to depolymerize lignin at various concentrations. Interestingly, a direct correlation was observed between lignin depolymerization and H2O2 concentration, while Fourier-transform infrared spectroscopy data revealed a constant disruption of the lignin structure accurately in the wavenumber region of 1000–1750 cm–1 irrespective of the H2O2 concentration. Carboxylic acid derivatives, benzopyran, hexanoic acid, and other valuable compounds were detected in the LC QTOF MS data from the depolymerized lignin mixture. Remarkably, SEM analysis demonstrated morphological changes in depolymerized lignin induced by the oxidative effects of hydroxyl radicals. Biochemical oxygen demand and chemical oxygen demand removal was 60 ± 3–85 ± 1% in anodic wastewater treatment. This research provides a sustainable and efficient technique for lignin valorization and wastewater treatment.

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Section: ■ Introductionsupporting

confidence: 58%

Section: Resultsmentioning

confidence: 99%

Exploring the Oxidative Effects of the Microbial Electro-Fenton Process on the Depolymerization of Lignin Extracted from Rice Straw in a Bio-Electrochemical System Coupled with Wastewater Treatment

Chang

Gupta

2023

Biomacromolecules

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“…In contrast, lignin is depolymerized in an MPPC system using only H 2 O 2 , a green oxidant that degrades quickly and does not need to be extensively purified to produce products with value added. Microbial peroxide‐producing cell systems provide a sustainable and environmentally friendly technique for depolymerizing lignin to its aromatic monomers, and the depolymerization is still much faster and tends to involve fewer complexities than biological depolymerization by the action of microorganisms 11 . The objective of this research was to evaluate the effect of fed‐batch mode over the batch mode for lignin depolymerization and its valorization to obtain platform chemicals of high commercial value with synchronized wastewater treatment in a microbial peroxide‐producing cell, including the effect of increasing lignin concentration in fed‐batch operation on MPPC performance, and also to analyze the performance of anodic microbes existing in wastewater via electricity generation.…”

Section: Introductionmentioning

confidence: 99%

Valorization of lignin to obtain platform chemicals via bio‐electrochemical systems: batch and fed‐batch mode analysis

Chang

Gupta

2023

J of Chemical Tech & Biotech

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BACKGROUND: There is tremendous potential for reusing lignin, which is generally discarded as waste. This research analyses the batch and fed-batch mode of the microbial peroxide-producing cell (MPPC), a type of bio-electrochemical cell that produces H 2 O 2 , a potent green oxidant utilized in the oxidation of Kraft lignin to produce platform chemicals with simultaneous wastewater treatment.RESULTS: The batch mode MPPC had a total shelf life of 10 days, with a peak phase of 5 days and maximum H 2 O 2 of 9.7 ± 0.06 mmol L −1 , and voltage of 164 ± 0.021 mV. The intermittent nutrition feeding strategy of fed-batch MPPCs aided microbe metabolic reactions, extending the system's lifespan to 21 days with a maximum voltage of 294 ± 0.01 to 525 ± 0.008 mV and H 2 O 2 of 14.14 ± 0.12 to 32.96 ± 1.35 mmol L −1 , respectively. This offered more exposure time for lignin hydroxyl radical oxidation by H 2 O 2 , with enhanced depolymerization of 37-80% of high lignin concentration in repeated cycles compared to batch mode, which accomplished only 53.46%. Fourier transform infrared spectroscopy confirmed the structural changes in lignin of all systems, displaying loss and disorientation of major functional groups with greater intensity in fed batch-operated MPPCs. Platform chemicals with high commercial value, including guaiacol, ferulic acid, vanillin, and others, were identified using liquid chromatography-mass spectrometry. In terms of wastewater treatment, biochemical oxygen demand and chemical oxygen demand removal efficiency ranged from 59% to 83%, with fed-batch being the most efficient.CONCLUSION: This research suggests that fed-batch mode MPPC is significantly more productive than batch mode MPPC for lignin valorization to produce platform chemicals, making it more sustainable, economical, and environmentally friendly.

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“…Laccase exhibits the ability to reduce oxygen molecules, leading to the one-electron oxidation of antibiotics (Piontek et al 2002). Moreover, recent investigations have demonstrated the efficacy of microbial laccase in degrading β-lactam drugs, attributing this capability to its potential for β-lactam ring deformation (Mukhopadhyay et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Electricity generation in a ceramic separator microbial fuel cell employing a bacterial consortium for penicillin removal

2023

Archives of Environmental Protection

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The contamination of the environment by antibiotics has become a serious problem, supported by abundant scientific evidence of its negative impact on both aquatic ecosystems and human health. Therefore, it is crucial to intensify research efforts towards developing effective and efficient processes for removing antibiotics from the aquatic environment. In this study, a bacterial consortium capable of breaking down penicillin was employed in a ceramic separator microbial fuel cell (MFC) to generate electricity. The consortium's properties such as laccase activity, penicillin removal and microbial structure were studied. The SF11 bacterial consortium, with a laccase activity of 6.16±0.04 U/mL, was found to be effective in breaking down penicillin. The highest rate of penicillin removal (92.15±0.27%) was achieved when the SF11 consortium was incubated at 30 °C for 48 hours. Furthermore, when used as a whole-cell biocatalyst in a low-cost upflow MFC, the Morganella morganii-rich SF11 consortium demonstrated the highest voltage and power density of 964.93±1.86 mV and 0.56±0.00 W/m3, respectively. These results suggest that the SF11 bacterial consortium has the potential for use in ceramic separator MFCs for the removal of penicillin and electricity generation.

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Degradation of β-lactam antibiotic ampicillin using sustainable microbial peroxide producing cell system

Cited by 10 publications

References 45 publications

Exploring the Oxidative Effects of the Microbial Electro-Fenton Process on the Depolymerization of Lignin Extracted from Rice Straw in a Bio-Electrochemical System Coupled with Wastewater Treatment

Exploring the Oxidative Effects of the Microbial Electro-Fenton Process on the Depolymerization of Lignin Extracted from Rice Straw in a Bio-Electrochemical System Coupled with Wastewater Treatment

Valorization of lignin to obtain platform chemicals via bio‐electrochemical systems: batch and fed‐batch mode analysis

Electricity generation in a ceramic separator microbial fuel cell employing a bacterial consortium for penicillin removal

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