Degradation of trichloroethylene and trans-1,2-dichloroethylene by a methanotrophic consortium in a fixed-film, packed-bed bioreactor

Strandberg, G.W.; Donaldson, T.L.; Farr, L.L.

doi:10.1021/es00069a016

Cited by 81 publications

(43 citation statements)

References 3 publications

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“…2B). Similar results were observed in other studies (12,13), where degradation of trichloroethylene became less efficient at low trichloroethylene concentrations. No volatile intermediates of trichloroethylene degradation were detected in either of the reactors during the time-course of the experiment.…”

Section: Resultssupporting

confidence: 92%

“…Decreased rates of trichloroethylene observed in reactor 2 after 8 d agreed well with other studies (12,13). These studies have reported a decrease in trichloroethylene degradation rates as concentrations of trichloroethylene decreased to approx 500 #g/L.…”

Section: Resultssupporting

confidence: 91%

“…The methanotrophic bacteria are known to cometabolize trichloroethylene, the soluble methane monooxygenase playing a major role in this step of cometabolism (6). Biodegradation of chlorinated alkenes has been reported by cultures stimulated with methane or natural gas (6,7,10,13). This study suggests that propane may also play an important role in promoting trichloroethylene degradation.…”

Section: Resultssupporting

confidence: 54%

“…Biodegradation of trichloroethylene has been shown to be stimulated by methane and propane (7,(10)(11)(12)(13)(14). Bacterial cultures capable of degrading aromatics have been well documented (14)(15)(16)(17)(18)(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of chlorinated aliphatics and aromatic compounds in total-recycle expanded-bed biofilm reactors

Korde

Phelps

Bienkowski

et al. 1993

Appl Biochem Biotechnol

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Ground-water contamination by chlorinated aliphafic compounds is a major cause for concern because of their toxicity. This study examined the biodegradation of trichloroethylene and aromatic compounds by microbial consortia enriched from contaminated subsurface sediments. The consortia were capable of utilizing methane and propane as sources of carbon and energy. Two continuously recycled expanded-bed bioreactors were inoculated with (1) the subsurface consortium, and (2) P. fluorescence, P. putida (strains pRB1401 and pWWO), and M. trichosporium OB3b. An uninoculated reactor containing 0.2% sodium azide and 0.5% formalin served as the control. Methane (5% v/v) and propane (3% v/v) were maintained by batch feeding through the course of the experiment. Greater than 97% degradation of trichloroethylene was observed over a period of 12 d. More than 99% of benzene, toluene, and xylene were degraded within the first 7 d. Dissolved oxygen levels were measured and found to be in the range 4.9-6.5 mg/L throughout the experiments.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Biodegradation of chlorinated aliphatics and aromatic compounds in total-recycle expanded-bed biofilm reactors

Korde

Phelps

Bienkowski

et al. 1993

Appl Biochem Biotechnol

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“…The ability to degrade TCE has been observed in both pure cultures and mixed microbial consortia (1,2,(5)(6)(7)(8)(9)(10)(11)(12)(13). Methanotrophs show the ability to degrade TCE and other chlorinated compounds (2,5).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of chlorinated aliphatic hydrocarbon mixtures in a single-pass packed-bed reactor

Lackey

Phelps

Bienkowski

et al. 1993

Appl Biochem Biotechnol

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Aliphatic chlorinated compounds, such as trichloroethylene (TCE) and tetrachloroethylene (PCE), are major contaminants of ground water. A single-pass packed-bed bioreactor was utilized to study the biodegradation of organic waste mixtures consisting of PCE, TCE, and other short-chain chlorinated organics. The bioreactor consisted of two 1960-mL glass columns joined in a series. One column was packed with sand containing a microbial consortia enriched from a contaminated site. The other column provided a reservoir for oxygen and a carbon source of methane/propane that was recirculated through the reactor. Sampling was accomplished by both direct headspace and liquid effluent concentration analyses. The reactor was operated in a single-pass mode. Greater than 99% degradation of trichloroethylene, approaching drinking water standards, was observed when the bioreactor residence time ranged from 1.9 to 3.2 d. Typically, when the reactor was pulse-fed with methane, propane, and air, 1 mol of TCE was degraded/110 mol of substrate utilized. Perturbation studies were performed to characterize reactor behavior. The system's degradation behavior was affected by providing different carbon sources, a pulse feeding regime, supplementing microbial biomass, and by altering flow rates.

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Laboratory evaluation of a two-stage treatment system for TCE cometabolism by a methane-oxidizing mixed culture

Smith

McCarty

1997

Biotechnol. Bioeng.

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Degradation of trichloroethylene and trans-1,2-dichloroethylene by a methanotrophic consortium in a fixed-film, packed-bed bioreactor

Cited by 81 publications

References 3 publications

Biodegradation of chlorinated aliphatics and aromatic compounds in total-recycle expanded-bed biofilm reactors

Biodegradation of chlorinated aliphatics and aromatic compounds in total-recycle expanded-bed biofilm reactors

Biodegradation of chlorinated aliphatic hydrocarbon mixtures in a single-pass packed-bed reactor

Laboratory evaluation of a two-stage treatment system for TCE cometabolism by a methane-oxidizing mixed culture

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