Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases

Kong, Lingyao; Cheng, Jinkui; Zhu, Yujuan; Ding, Yanglin; Meng, Jingjing; Chen, Zhizhong; Xie, Qi; Guo, Yan; Li, Jigang; Gong, Zhizhong

doi:10.1038/ncomms9630

Cited by 245 publications

(278 citation statements)

References 61 publications

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“…For example, BRASSINOSTEROID INSENSITIVE2 was found to positively modulate ABA signaling through phosphorylation of subgroup III SnRKs in Arabidopsis (Cai et al, 2014), and ARK plays an essential role in the activation of SnRK2 in the moss Physcomitrella patens (Cai et al, 2014;Saruhashi et al, 2015). Recently, ABI1 was found to be degraded through ubiquitination by PUB12 and PUB13 in the presence of ABA and an ABA receptor in Arabidopsis, which may be regarded, to our knowledge, as a new positive regulation pathway of ABA signaling (Kong et al, 2015).…”

Section: Osbzip23 Positively Regulates the Aba Level In Ricementioning

confidence: 93%

Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes

et al. 2016

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The OsbZIP23 transcription factor has been characterized for its essential role in drought resistance in rice (Oryza sativa), but the mechanism is unknown. In this study, we first investigated the transcriptional activation of OsbZIP23. A homolog of SnRK2 protein kinase (SAPK2) was found to interact with and phosphorylate OsbZIP23 for its transcriptional activation. SAPK2 also interacted with OsPP2C49, an ABI1 homolog, which deactivated the SAPK2 to inhibit the transcriptional activation activity of OsbZIP23. Next, we performed genome-wide identification of OsbZIP23 targets by immunoprecipitation sequencing and RNA sequencing analyses in the OsbZIP23-overexpression, osbzip23 mutant, and wild-type rice under normal and drought stress conditions. OsbZIP23 directly regulates a large number of reported genes that function in stress response, hormone signaling, and developmental processes. Among these targets, we found that OsbZIP23 could positively regulate OsPP2C49, and overexpression of OsPP2C49 in rice resulted in significantly decreased sensitivity of the abscisic acid (ABA) response and rapid dehydration. Moreover, OsNCED4 (9-cis-epoxycarotenoid dioxygenase4), a key gene in ABA biosynthesis, was also positively regulated by OsbZIP23. Together, our results suggest that OsbZIP23 acts as a central regulator in ABA signaling and biosynthesis, and drought resistance in rice.

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Section: Osbzip23 Positively Regulates the Aba Level In Ricementioning

confidence: 93%

Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes

et al. 2016

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“…T-DNA insertion mutants of CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR ( CAMTA ) 3 displayed enhanced disease resistance and dwarfism when grown at low temperatures (Du et al, 2009), whereas a gain-of-function mutation in CAMTA3 leads to compromised immune responses (Jing et al, 2011; Nie et al, 2012). PLANT U-BOX (PUB) 13 is a E3 ubiquitin ligase involved in degradation of FLAGELLIN-SENSITIVE2 and the ABA co-receptor ABI1 (Lu et al, 2011; Kong et al, 2015). Knockout mutant plants of pub13 exhibit spontaneous cell death and enhanced pathogen resistance (Li et al, 2012).…”

Section: Autoimmunity Caused By Mutations In Other Types Of Proteinmentioning

confidence: 99%

Mighty Dwarfs: Arabidopsis Autoimmune Mutants and Their Usages in Genetic Dissection of Plant Immunity

2016

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Plants lack the adaptive immune system possessed by mammals. Instead they rely on innate immunity to defend against pathogen attacks. Genomes of higher plants encode a large number of plant immune receptors belonging to different protein families, which are involved in the detection of pathogens and activation of downstream defense pathways. Plant immunity is tightly controlled to avoid activation of defense responses in the absence of pathogens, as failure to do so can lead to autoimmunity that compromises plant growth and development. Many autoimmune mutants have been reported, most of which are associated with dwarfism and often spontaneous cell death. In this review, we summarize previously reported Arabidopsis autoimmune mutants, categorizing them based on their functional groups. We also discuss how their obvious morphological phenotypes make them ideal tools for epistatic analysis and suppressor screens, and summarize genetic screens that have been carried out in various autoimmune mutant backgrounds.

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“…The E3 ligases PUB12 and PUB13 both work to attenuate PTI by ubiquitinating the flg22 receptor FLS2, facilitating its degradation (Lu et al, 2011). Recent Y2H screens have revealed potential coregulatory partners and substrates for ubiquitination by PUB13, including phosphatidylinositol-4 kinase and RABA4B, with which it complexes to negatively regulate salicylic acid-mediated defenses (Antignani et al, 2015), and the abscisic acid regulator ABI1, a PP2C family member, which is a PUB13 substrate for ubiquitination and degradation (Kong et al, 2015). In addition, PUB22, PUB23, and PUB24 also suppress immunity.…”

Section: Stnrl1 Overexpression Suppresses Inf1-mediated Cell Death Anmentioning

confidence: 99%

Potato NPH3/RPT2-Like Protein StNRL1, Targeted by a Phytophthora infestans RXLR Effector, Is a Susceptibility Factor

et al. 2016

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Plant pathogens deliver effectors to manipulate host processes. We know little about how fungal and oomycete effectors target host proteins to promote susceptibility, yet such knowledge is vital to understand crop disease. We show that either transient expression in Nicotiana benthamiana, or stable transgenic expression in potato (Solanum tuberosum), of the Phytophthora infestans RXLR effector Pi02860 enhances leaf colonization by the pathogen. Expression of Pi02860 also attenuates cell death triggered by the P. infestans microbeassociated molecular pattern INF1, indicating that the effector suppresses pattern-triggered immunity. However, the effector does not attenuate cell death triggered by Cf4/Avr4 coexpression, showing that it does not suppress all cell death activated by cell surface receptors. Pi02860 interacts in yeast two-hybrid assays with potato NPH3/RPT2-LIKE1 (NRL1), a predicted CULLIN3-associated ubiquitin E3 ligase. Interaction of Pi02860 in planta was confirmed by coimmunoprecipitation and bimolecular fluorescence complementation assays. Virus-induced gene silencing of NRL1 in N. benthamiana resulted in reduced P. infestans colonization and accelerated INF1-mediated cell death, indicating that this host protein acts as a negative regulator of immunity. Moreover, whereas NRL1 virus-induced gene silencing had no effect on the ability of the P. infestans effector Avr3a to suppress INF1-mediated cell death, such suppression by Pi02860 was significantly attenuated, indicating that this activity of Pi02860 is mediated by NRL1. Transient overexpression of NRL1 resulted in the suppression of INF1-mediated cell death and enhanced P. infestans leaf colonization, demonstrating that NRL1 acts as a susceptibility factor to promote late blight disease.

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Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases

Cited by 245 publications

References 61 publications

Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes

Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes

Mighty Dwarfs: Arabidopsis Autoimmune Mutants and Their Usages in Genetic Dissection of Plant Immunity

Potato NPH3/RPT2-Like Protein StNRL1, Targeted by a Phytophthora infestans RXLR Effector, Is a Susceptibility Factor

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