Degradation of raw corn stover powder (RCSP) by an enriched microbial consortium and its community structure

Degradation of Lignocellulosic Components in Un-pretreated Vinegar Residue Using an Artificially Constructed Fungal Consortium

“…Both the highest reducing sugar release and CMCase activity were observed after five days (Table 5). These results are similar to previous reports (Yang et al 2004;Feng et al 2011). These findings showed that aerobic metabolism prevailed during the first five days, and that, in subsequent fermentation stages, oxygen was limited and the reducing sugar was converted to other products (Feng et al 2011).…”

“…Cellulase activity was detected using sodium carboxymethyl cellulose (CMC) as the substrate, which is a soluble cellulose derivative, according to the method of Feng et al (2011). Xylanase activity was tested by the methodology described by Latif et al (2006).…”

Thermophilic and cellulolytic consortium isolated from composting plants improves anaerobic digestion of cellulosic biomass: Toward a microbial resource management approach

“…At the end of the culture, centrifugation (8000 x g) allowed separation of supernatant and pellet which included residual substrate and microbial biomass. Pellet was then suspended in 100 ml acetic acid/nitric acid reagent (Feng et al, 2011) and heated at 100 °C for 30 min to remove the biological cells. Then, acetic acid/nitric acid treated suspension was filtered.…”

“…The remaining cellulosic material was washed three times using 100 ml of distilled water each time. After washing and filtration, filtered solids were dried at 105 °C and determined gravimetrically (Feng et al, 2011). Degradation ratio was calculated according to the equation:…”

“…As well in aerobic conditions (Wongwilaiwalin et al, 2010) as in anaerobic conditions (Izquierdo et al, 2010) compost is a frequent microbial resource for enrichment culture. Nevertheless, other types of inocula have been tested for the development of efficient microbial cellulolytic communities, such as forest soil (Feng et al, 2011), or anaerobic digester sludge (Yan et al, 2012). Accordingly, based on enrichment method from compost samples, the present work has led to the design of an anaerobic thermophilic cellulolytic microbial consortium able to degrade cellulose under conditions related to anaerobic digestion process.…”