Degradation of polyphosphates by polyphosphate kinases from Ruegeria pomeroyi

Achbergerová, Lucia; Nahálka, Jozef

doi:10.1007/s10529-014-1566-6

Cited by 23 publications

(21 citation statements)

References 10 publications

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“…It is affiliated within the marine Roseobacter clade, demonstrating its seawater habitat (Sonnenschein et al ., 2017; Kim et al ., 2019; Kitamura et al ., 2019). Furthermore, it was shown to utilize proteins and degrade triester compounds in aquatic environments and could produce secondary metabolites such as antibiotics (Porsby et al ., 2008; Achbergerova and Nahalka, 2014; Yamaguchi et al ., 2016). Thus, the digestive enzymes produced by Vibrio and Ruegeria in SW and SFW may indicate a higher metabolic rate in high salinity environments, and their growth and survival may require particular molecules in the seawater.…”

Section: Discussionmentioning

confidence: 99%

Osmotic stress induces gut microbiota community shift in fish

Lai

Lin

Tam

et al. 2020

Environmental Microbiology

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Summary Alteration of the gut microbiota plays an important role in animal health and metabolic diseases. However, little is known with respect to the influence of environmental osmolality on the gut microbial community. The aim of the current study was to determine whether the reduction in salinity affects the gut microbiota and identify its potential role in salinity acclimation. Using Oryzias melastigma as a model organism to perform progressive hypotonic transfer experiments, we evaluated three conditions: seawater control (SW), SW to 50% sea water transfer (SFW) and SW to SFW to freshwater transfer (FW). Our results showed that the SFW and FW transfer groups contained higher operational taxonomic unit microbiota diversities. The dominant bacteria in all conditions constituted the phylum Proteobacteria, with the majority in the SW and SFW transfer gut comprising Vibrio at the genus level, whereas this population was replaced by Pseudomonas in the FW transfer gut. Furthermore, our data revealed that the FW transfer gut microbiota exhibited a reduced renin–angiotensin system, which is important in SW acclimation. In addition, induced detoxification and immune mechanisms were found in the FW transfer gut microbiota. The shift of the bacteria community in different osmolality environments indicated possible roles of bacteria in facilitating host acclimation.

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Section: Discussionmentioning

confidence: 99%

Osmotic stress induces gut microbiota community shift in fish

Lai

Lin

Tam

et al. 2020

Environmental Microbiology

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“…Ruegeria and Shimia are both members of the Roseobacter clade, with essential metabolic capabilities, and are involved in protein utilization 38 . Ruegeria also have high phosphodiesterase and phosphomonoesterase activities that contribute to the degradation of phosphate triester compounds in aquatic environments 39,40 . Mameliella play important roles in the degradation of aromatic compounds 41,42 and can accumulate beneficial poly-β-hydroxybutyrate (PHB) granules in cells 43 .…”

Section: Discussionmentioning

confidence: 99%

Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch

Duan

Wang

Liu

et al. 2019

Sci Rep

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Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals. However, the roles of RS on shrimp intestine health is unknown. In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei . The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days. The results showed that dietary RS improved the morphology of the intestine mucosa. RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes ( proPO , ALF , Lys , HSP70 , Trx , Muc-1 , Muc-2 , Muc-5AC , Muc-5B , and Muc-19 ). A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial. Specifically, RS increased the abundances of Proteobacteria and decreased the abundance of Bacteroidetes. At the genus level, the beneficial bacteria ( Lutimonas , Ruegeria , Shimia , Mesoflavibacter , and Mameliella ) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens ( Formosa and Pseudoalteromonas ) were decreased in response to dietary RS. Our results revealed that dietary RS could improve the intestine health of L . vannamei , probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.

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“…RpPPK2-3 uses polyphosphate (PolyP) as an economic donor to convert nucleoside diphosphates (NDPs) to NTPs. [16][17][18] We here evaluate the efficiency of the enzyme for ATP and UTP regeneration as well as the combination of UDP-sugar and HA synthesis through analysis with multiplexed capillary electrophoreses (MP-CE).…”

Section: Introductionmentioning

confidence: 99%

Integration of a Nucleoside Triphosphate Regeneration System in the One‐pot Synthesis of UDP‐sugars and Hyaluronic Acid

et al. 2021

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Enzymatic synthesis is a promising approach to produce hyaluronic acid (HA) products with defined molecular weight (MW) and dispersity. We here report on the evaluation of nucleoside triphosphate (NTP) regeneration system for the synthesis of the precursors uridine-5'-diphosphate α-d-N-acetylglucosamine (UDP-GlcNAc) and uridine-5'-diphosphate α-dglucuronic acid (UDP-GlcA) and integration in the one-pot synthesis of HA. By integration of polyphosphate kinase from Ruegeria pomeroyi (RpPPK2-3) into the enzyme cascade to synthesize UDP-GlcA we reached 89 % yield after 1 h with a regeneration cycle number (RCN) of 17. With the UDP-GlcNAc cascade, the ATP concentration was lowered 250-fold and reached 94 % yield after 1 h with an RCN of 234. Integration of the RpPPK2-3 regeneration system in one-pot HA synthesis afforded optimization of MgCl 2 and ATP starting concentrations. With 25 mM MgCl 2 and 0.1 mM ATP, a HA concentration of 0.81 g L À 1 with an average MW of 1.17 MDa and dispersity of about 1.08 and an RCN of 75 was obtained. We conclude that integration of NTP regeneration with RpPPK2-3 expanded the enzymatic possibilities to produce HA with lower range MW.

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Degradation of polyphosphates by polyphosphate kinases from Ruegeria pomeroyi

Cited by 23 publications

References 10 publications

Osmotic stress induces gut microbiota community shift in fish

Osmotic stress induces gut microbiota community shift in fish

Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch

Integration of a Nucleoside Triphosphate Regeneration System in the One‐pot Synthesis of UDP‐sugars and Hyaluronic Acid

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