Degradation of Phytochrome Interacting Factor 3 in Phytochrome-Mediated Light Signaling

Park, Eunae; Kim, Jong-Hyun; Lee, Yeon; Shin, Jieun; Oh, Eunkyoo; Chung, Won Sang; Liu, Jang Ryul; Choi, Giltsu

doi:10.1093/pcp/pch125

Cited by 194 publications

(175 citation statements)

References 46 publications

Supporting

Mentioning

163

Contrasting

Unclassified

Order By: Relevance

“…This pattern of light regulation was specifically induced by R but not FR light (Figure 4c). The light-regulated protein abundance of PIF4-HA and PIF5-HA was thus similar to that reported for their close relatives PIF1 and PIF3, except that PIF4 and PIF5 re-accumulated much faster than PIF1 after return into darkness and FR light did not induce a significant decrease in PIF4 and PIF5 (Figure 4c and Supplementary Figure S2c, and data not shown) (Al-Sady et al, 2006;Bauer et al, 2004;Monte et al, 2004;Park et al, 2004;Shen et al, 2005;Viczian et al, 2005).…”

Section: Pif4 and Pif5 Promote The Shade-avoidance Responsesupporting

confidence: 81%

“…A similar mechanism operates for PIF3; moreover, PIF3 degradation requires the interaction with phyB through a functional APB domain (Al-Sady et al, 2006). In both cases, the degradation of these transcription factors is proteasome-mediated (Al-Sady et al, 2006;Bauer et al, 2004;Monte et al, 2004;Oh et al, 2006;Park et al, 2004;Shen et al, 2005;Viczian et al, 2005).…”

Section: Introductionmentioning

confidence: 93%

“…The rapid decrease in PIF4 and PIF5 abundance in response to a R-light treatment, and the precedent for PIF1 and PIF3 light regulation, suggested involvement of the proteasome in the regulation of PIF4 and PIF5 (Bauer et al, 2004;Monte et al, 2004;Park et al, 2004;Shen et al, 2005). To test this directly, etiolated seedlings either kept in the dark or transferred into R light were treated with proteasome inhibitors.…”

Section: Pif5 Protein Accumulation Is Controlled By R/fr Ratiomentioning

confidence: 99%

See 2 more Smart Citations

Phytochrome‐mediated inhibition of shade avoidance involves degradation of growth‐promoting bHLH transcription factors

Lorrain¹,

Allen²,

Duek³

et al. 2007

The Plant Journal

672

848

View full text Add to dashboard Cite

SummaryPlant growth and development are particularly sensitive to changes in the light environment and especially to vegetational shading. The shade-avoidance response is mainly controlled by the phytochrome photoreceptors. In Arabidopsis, recent studies have identified several related bHLH class transcription factors (PIF, for phytochrome-interacting factors) as important components in phytochrome signaling. In addition to a related bHLH domain, most of the PIFs contain an active phytochrome binding (APB) domain that mediates their interaction with light-activated phytochrome B (phyB). Here we show that PIF4 and PIF5 act early in the phytochrome signaling pathways to promote the shade-avoidance response. PIF4 and PIF5 accumulate to high levels in the dark, are selectively degraded in response to red light, and remain at high levels under shademimicking conditions. Degradation of these transcription factors is preceded by phosphorylation, requires the APB domain and is sensitive to inhibitors of the proteasome, suggesting that PIF4 and PIF5 are degraded upon interaction with light-activated phyB. Our data suggest that, in dense vegetation, which is rich in far-red light, shade avoidance is triggered, at least partially, as a consequence of reduced phytochrome-mediated degradation of transcription factors such as PIF4 and PIF5. Consistent with this idea, the constitutive shadeavoidance phenotype of phyB mutants partially reverts in the absence of PIF4 and PIF5.

show abstract

Section: Pif4 and Pif5 Promote The Shade-avoidance Responsesupporting

confidence: 81%

Section: Introductionmentioning

confidence: 93%

Section: Pif5 Protein Accumulation Is Controlled By R/fr Ratiomentioning

confidence: 99%

See 1 more Smart Citation

Phytochrome‐mediated inhibition of shade avoidance involves degradation of growth‐promoting bHLH transcription factors

Lorrain¹,

Allen²,

Duek³

et al. 2007

The Plant Journal

672

848

View full text Add to dashboard Cite

show abstract

“…The striking constitutively photomorphogeniclike phenotype of quadruple pif1 pif3 pif4 pif5 (pifq) mutant seedlings grown in complete darkness (Leivar et al, 2008a) has provided compelling evidence that these PIF family members function collectively, in a partially redundant or overlapping fashion, to constitutively promote skotomorphogenesis (repress photomorphogenesis) in young dark-grown seedlings (Leivar et al, 2008a;Shin et al, 2009). Additional evidence shows that photoactivated phy reverses this promotion/repression, upon initial exposure to light, by inducing rapid degradation of the PIF molecules, via the ubiquitin-proteasome system (Bauer et al, 2004;Park et al, 2004;Shen et al, 2005Shen et al, , 2008Oh et al, 2006;Nozue et al, 2007;Shen et al, 2007;Lorrain et al, 2008). This process involves rapid, phy-induced phosphorylation of the interacting bHLH protein in the nucleus, as a prelude to ubiquitin-proteasome system-mediated proteolysis (Al-Sady et al, 2006Shen et al, 2007Shen et al, , 2008Lorrain et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Dynamic Antagonism between Phytochromes and PIF Family Basic Helix-Loop-Helix Factors Induces Selective Reciprocal Responses to Light and Shade in a Rapidly Responsive Transcriptional Network in Arabidopsis

et al. 2012

View full text Add to dashboard Cite

“…These analyses revealed that the individual family members have, at least in part, redundant functions (Castillon et al 2007; Leivar et al 2008). PIF function is also negatively regulated by interactions with light-activated phytochrome B that induce PIF proteolysis in the light (Bauer et al 2004;Park et al 2004;Shen et al 2005). PIFs thus integrate light and GA signals to control developmental responses.…”

mentioning

confidence: 99%

The GATA-type transcription factors GNC and GNL/CGA1 repress gibberellin signaling downstream from DELLA proteins and PHYTOCHROME-INTERACTING FACTORS

Richter

Behringer²,

Müller³

et al. 2010

Genes Dev.

198

309

View full text Add to dashboard Cite

The phytohormone gibberellin (GA) regulates various developmental processes in plants such as germination, greening, elongation growth, and flowering time. DELLA proteins, which are degraded in response to GA, repress GA signaling by inhibitory interactions with PHYTOCHROME-INTERACTING FACTOR (PIF) family transcription factors. How GA signaling is controlled downstream from the DELLA and PIF regulators is, at present, unclear. Here, we characterize GNC (GATA, NITRATE-INDUCIBLE, CARBON-METABOLISM INVOLVED) and GNL/CGA1 (GNC-LIKE/CYTOKININ-RESPONSIVE GATA FACTOR1), two homologous GATA-type transcription factors from Arabidopsis thaliana that we initially identified as GA-regulated genes. Our genetic analyses of loss-of-function mutants and overexpression lines establish that GNC and GNL are functionally redundant regulators of germination, greening, elongation growth and flowering time. We further show by chromatin immunoprecipitation that both genes are potentially direct transcription targets of PIF transcription factors, and that their expression is up-regulated in pif mutant backgrounds. In line with a key role of GNC or GNL downstream from DELLA and PIF signaling, we find that their overexpression leads to gene expression changes that largely resemble those observed in a ga1 biosynthesis mutant or a pif quadruple mutant. These findings, together with the fact that gnc and gnl loss-of-function mutations suppress ga1 phenotypes, support the hypothesis that GNC and GNL are important repressors of GA signaling downstream from the DELLA and PIF regulators.[Keywords: Arabidopsis thaliana; DELLA protein; GATA factor; gibberellin; LLM domain; phytochrome-interacting factor; PIF] Supplemental material is available at http://www.genesdev.org.

show abstract

Degradation of Phytochrome Interacting Factor 3 in Phytochrome-Mediated Light Signaling

Cited by 194 publications

References 46 publications

Phytochrome‐mediated inhibition of shade avoidance involves degradation of growth‐promoting bHLH transcription factors

Phytochrome‐mediated inhibition of shade avoidance involves degradation of growth‐promoting bHLH transcription factors

Dynamic Antagonism between Phytochromes and PIF Family Basic Helix-Loop-Helix Factors Induces Selective Reciprocal Responses to Light and Shade in a Rapidly Responsive Transcriptional Network in Arabidopsis

The GATA-type transcription factors GNC and GNL/CGA1 repress gibberellin signaling downstream from DELLA proteins and PHYTOCHROME-INTERACTING FACTORS

Contact Info

Product

Resources

About