Degradation of pharmaceuticals in non-sterile urban wastewater by Trametes versicolor in a fluidized bed bioreactor

Cruz-Morató, Carles; Ferrando-Climent, Laura; Rodríguez-Mozaz, Sara; Barceló, Damià; Marco‐Urrea, Ernest; Vicent, Teresa; Sarrà, Montserrat

doi:10.1016/j.watres.2013.06.007

Cited by 194 publications

(121 citation statements)

References 41 publications

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“…Laccase activity was determined spectrophotometrically in the culture supernatant obtained by filtering through 0.45 μm syringe filters and measured by monitoring the oxidation of 2,6-dimethoxiphenol (DMP) as described before [44]. The enzyme activity was expressed in enzyme units (U: micromoles/min).…”

Section: Discussionmentioning

confidence: 99%

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Zahmatkesh

Spanjers

Lier

2017

Environmental Technology

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Citation (APA)Zahmatkesh, M., Spanjers, H., & van Lier, J. B. (2017). A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum. Environmental Technology, 1-11. https://doi.org/10.1080/09593330.2017.1347718 Important noteTo cite this publication, please use the final published version (if applicable). Please check the document version above. CopyrightOther than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons. Takedown policyPlease contact us and provide details if you believe this document breaches copyrights. We will remove access to the work immediately and investigate your claim. In this study, we tested a new approach to facilitate the application of white rot fungi (WRF) under non-sterile conditions, by introducing grain sorghum as carrier and sole carbon and nutrient source for WRF. To this end, Trametes versicolor was immobilized on sorghum, and its ability to remove humic acid (HA) from synthetic and real industrial wastewater was studied. HA removal was measured as colour reduction and also analysed via size exclusion chromatography (SEC). Under sterile conditions, 80% colour removal was achieved for both synthetic and real wastewater using immobilized WRF on sorghum, without adding any additional carbon or nutrient sources. Under non-sterile conditions, immobilized fungi could again remove 80% of the colour and reached a maximum of 40 U/L laccase activity. In contrast, non-immobilized fungi cultivated in non-sterile wastewater supplemented with additional nutrients, reached only 10% decolourization and maximum 5 U/L laccase activity. SEC analysis showed that bioremoval of HA by WRF was associated with degradation of HA. Finally, immobilized fungi were used to treat real wastewater, under non-sterile conditions, in a sequential batch order without renewing the immobilized fungi. Four batch feedings were conducted and 80%, 70%, 50% and 40% colour removal was achieved for each batch, respectively, over a total incubation period of 19 days.

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Section: Discussionmentioning

confidence: 99%

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Zahmatkesh

Spanjers

Lier

2017

Environmental Technology

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“…An improved version of the fluidized-bed is the pulsing bioreactor, which avoids the drawbacks of conventional fluidized bed reactors by controlling the pellet growth with a system that allows not only to maintain active biomass, but also to "shave" the fungal biomass when the desired thickness has been exceeded [77]. The formation of compact pellets as spheres is promoted by the air pulses [42], along with their homogeneous distribution in the reactor [90]. The fluidized bed reactor has been used for the removal of pharmaceutical active compounds [55,69,89,90], endocrine disrupting contaminants [91], dyes [92], colourants, COD, etc, among others [59,93].…”

Section: Fluidized Bed Reactormentioning

confidence: 99%

Fungal pelleted reactors in wastewater treatment: Applications and perspectives

Espinosa-Ortiz

Rene

Pakshirajan

et al. 2016

Chemical Engineering Journal

197

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“…This suggested that growth on poplar wood significantly altered T. versicolor's expression of enzymes that hydrolyze methyl and benzoic acid groups. Catechol and benzoate were important products in lignin degradation (Aranda et al 2010;Sari et al2012;Cruz-Morató et al 2013;Anasonye et al 2014), but were also produced during aminobenzoate degradation. From these results it was inferred that lignin degradation may have been related to aminobenzoate degradation or have similar intermediate compounds.…”

Section: Validation Of the Gene Expression Profiles By Qrt-pcrmentioning

confidence: 99%

Transcriptomic Profile of Lignocellulose Degradation from Trametes versicolor on Poplar Wood

Zhang

Wang

et al. 2017

BioResources

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The Trametes versicolor genome is predicted to encode many enzymes that effectively degrade lignin, making it a potentially useful tool for biopulping. However, the wood degradation mechanism of T. versicolor is not clear. To identify the enzymes that contribute to lignocellulose degradation, changes in the T. versicolor transcriptome during growth on poplar wood, relative to growth on glucose medium, were evaluated. Eight hundred and fifty-three genes were differentially expressed, with 360 genes up-regulated and 493 genes were down-regulated on poplar wood. Notably, most genes involved in lignin degradation were upregulated, including eight lignin peroxidase (LiP) genes. Genes encoding cellulose and hemicellulose degrading-enzymes were mostly downregulated, including six endo-β-1,4-glucanase genes and three cellobiohydrolase I genes. These results characterized transcriptomic changes related to lignocellulose degradation. This information could be used to develop T. versicolor as a tool to improve the efficiency of lignin degradation or to provide a theoretical foundation for a new paper pulp manufacturing process.

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Degradation of pharmaceuticals in non-sterile urban wastewater by Trametes versicolor in a fluidized bed bioreactor

Cited by 194 publications

References 41 publications

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Fungal pelleted reactors in wastewater treatment: Applications and perspectives

Transcriptomic Profile of Lignocellulose Degradation from Trametes versicolor on Poplar Wood

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