Degradation of pentachlorophenol by polyurethane-immobilized Flavobacterium cells

O’Reilly, Kirk T.; Crawford, Ronald L.

doi:10.1128/aem.55.9.2113-2118.1989

Cited by 121 publications

(32 citation statements)

References 22 publications

(22 reference statements)

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“…At least, during the detoxification of 0.5 g/l of MCPB in the PU-bubble reactor the maximum degradation rate of MCPB increased up to Q,,, = 130 pmoles/l * h, in contrast to the reactor failure in the experiment without the PU-supply. A similar protective effect of another type of PU-foam (2% w/v) was reported for Flavobacterium ATCC 39723 concerning pentachlorophenol (O'REILLY and CRAWFORD 1989). Thus, due to the reversible binding of this substance 0.3 g/l of pentachlorophenol was found to be mineralized by PU-immobilized cells, while only 0.15 g/1 were degraded without PU.…”

Section: Discussionsupporting

confidence: 76%

“…In the PU-supplied experiments the increase of the viable cell number of strain MH was always less than without PU due to a kind of association of the cells with the PU-foam. Thus, by the use of this PU-carrier no active immobilization procedure prior to the degradation experiments is required, in contrast to the operation with cells immobilized within the PU-foam (O'REILLY and CRAWFORD 1989) or with calcium alginate-encapsulated cells (REHG et al 1986, FERSCHL et al 1991. Furthermore, such PU-carriers are persistent to biodegradation, which is important for unsterile heterotrophic processes, whereas a lot of microorganisms are capable of utilizing natural polymers like alginate (KINOSHITA et al 199 1).…”

Section: Discussionmentioning

confidence: 99%

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Enhancement of the bacterial degradation of phenoxyalkanoate herbicides by the use of modified polyurethane foam as a support

Hinteregger

Loidl

Stockinger

et al. 1995

J. Basic Microbiol.

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The enhancement of the degradative behaviour of Flavobacterium sp. MH concerning the phenoxyalkanoate herbicides 2,4‐D, 2,4‐DP, 2,4‐DB, MCPA, MCPP, and MCPB, respectively, was achieved by the use of an inert polyurethane (PU)‐foam. In comparative batch‐degradation experiments with and without the addition of the PU‐carrier (1.25 % w/v), respectively, the advantages of the PU‐supply were demonstrated. When 2,4‐D, 2,4‐DP, and MCPP, respectively, was used as a growth substrate by strain MH the PU‐supply led to a reduced lag‐phase. In the case of the stronger toxic phenoxyalkanoates 2,4‐DB and MCPB, respectively, the inital substrate concentration (0.1 g/1) was significantly reduced to a minor toxic concentration due to a distinct adsorption to the PU‐carrier, thus enabling the biodegradation. Finally, the total initially supplied amount of the phenoxyalkanoates was detoxified as verified by thechloride balance. After several steps of subcultivation, even a five‐fold increased MCPB concentration (0.5 g/1) could be detoxified by strain MH in a PU‐supplied bubble reactor within several days.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Enhancement of the bacterial degradation of phenoxyalkanoate herbicides by the use of modified polyurethane foam as a support

Hinteregger

Loidl

Stockinger

et al. 1995

J. Basic Microbiol.

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“…Inoculation of pentachlorophenol-degrading bacteria to activated sludge or natural waters stimulated degradation of pentachlorophenol [389,390]. The use of immobilized chlorophenol degrading bacteria has been demonstrated in several studies [391][392][393][394][395][396]. Immobilization increases the tolerance of bacteria to toxic substrates.…”

Section: Bioremediation Of Contami-nated Soil and Groundwatermentioning

confidence: 99%

Microbial breakdown of halogenated aromatic pesticides and related compounds

Häggblom

1992

FEMS Microbiology Letters

363

132

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Considerable progress has been made in the last few years in understanding the mechanisms of microbial degradation of halogenated aromatic compounds. Much is already known about the degradation mechanisms under aerobic conditions, and metabolism under anaerobiosis has lately received increasing attention. The removal of the halogen substituent is a key step in degradation of halogenated aromatics. This may occur as an initial step via reductive, hydrolytic or oxygenolytic mechanisms, or after cleavage of the aromatic ring at a later stage of metabolism. In addition to degradation, several biotransformation reactions, such as methylation and polymerization, may take place and produce more toxic or recalcitrant metabolites. Studies with pure bacterial and fungal cultures have given detailed information on the biodegradation pathways of several halogenated aromatic compounds. Several of the key enzymes have been purified or studied in cell extracts, and there is an increasing understanding of the organization and regulation of the genes involved in haloaromatic degradation. This review will focus on the biodegradation and biotransformation pathways that have been established for halogenated phenols, phenoxyalkanoic acids, benzoic acids, benzenes, anilines and structurally related halogenated aromatic pesticides. There is a growing interest in developing microbiological methods for clean‐up of soil and water contaminated with halogenated aromatic compounds.

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“…Encapsulated bacteria are a safe and useful way to apply inoculants to soil environments in agriculture [20,21] and soil bioremediation [22,23]. During the early 1990s, reviews were published on methods of introducing bacteria into soil [24] and the use of solid carriers to introduce bacteria into soil [25].…”

Section: Introductionmentioning

confidence: 99%

“…In our laboratory, studies on the use of alginate and U-carrageenan as carriers for microbial inoculants with lac-lux-marked Pseudomonas aeruginosa UG2Lr showed that these microorganisms survived up to 360 days at 4³C [26]. Encapsulated bacterial cells have also been used in bioremediation of pentachlorophenol [23] and phenols [22].…”

Section: Introductionmentioning

confidence: 99%

Bacterial survival and mineralization of p-nitrophenol in soil by green fluorescent protein-marked Moraxella sp. G21 encapsulated cells

Errampalli

1999

FEMS Microbiology Ecology

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Moraxella sp. G21 cells marked with the green fluorescent protein (gfp) survived in U-carrageenan beads and as free cells for a month after inoculation into autoclaved soil and non-sterile soil contaminated with p-nitrophenol (PNP). Similar [U-14 C]PNP mineralization values were produced by encapsulated Moraxella sp. G21 cells and as free cells (53 and 60% mineralization). There was no significant difference between cell survival and [U-14 C]PNP mineralization activity in soil by the rifampicin-resistant Moraxella sp. mental strain and Moraxella sp. G21. The ability of encapsulated Moraxella sp. G21 cells to survive, retain their green fluorescence and mineralize [U-14 C]PNP suggests that the GFP-marked strain encapsulated in U-carrageenan may be useful for bioremediation of toxic chemicals in soil. ß

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Degradation of pentachlorophenol by polyurethane-immobilized Flavobacterium cells

Cited by 121 publications

References 22 publications

Enhancement of the bacterial degradation of phenoxyalkanoate herbicides by the use of modified polyurethane foam as a support

Enhancement of the bacterial degradation of phenoxyalkanoate herbicides by the use of modified polyurethane foam as a support

Microbial breakdown of halogenated aromatic pesticides and related compounds

Bacterial survival and mineralization of p-nitrophenol in soil by green fluorescent protein-marked Moraxella sp. G21 encapsulated cells

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