Degradation of PCB congeners by bacterial strains

Rein, Arno; Fernqvist, Margit M.; Mayer, Philipp; Trapp, Stefan; Bittens, Martin; Karlson, U.

doi:10.1007/s00253-007-1175-6

Cited by 37 publications

(17 citation statements)

References 42 publications

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“…In unplanted soil with bioaugmentation, PCB 77 and 153 removal was not improved (Figure 1). This observation is consistent with previous LB400 PCB biodegradation studies, where LB400 displayed a stronger capability to degrade PCB 52 than PCB 77 and 153 (Bedard et al, 1986; Bopp, 1986; Gibson et al, 1993; Rein et al, 2007). Compared with PCB 52, PCB 77 and 153 have higher K ow values and lower water solubilities (Van Noort et al, 2010).…”

Section: Resultssupporting

confidence: 92%

Enhanced polychlorinated biphenyl removal in a switchgrass rhizosphere by bioaugmentation with Burkholderia xenovorans LB400

Liang

Meggo

et al. 2014

Ecological Engineering

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Phytoremediation makes use of plants and associated microorganisms to clean up soils and sediments contaminated with inorganic and organic pollutants. In this study, switchgrass (Panicum virgatum) was used to test for its efficiency in improving the removal of three specific polychlorinated biphenyl (PCB) congeners (PCB 52, 77 and 153) in soil microcosms. The congeners were chosen for their ubiquity, toxicity, and recalcitrance. After 24 weeks of incubation, loss of 39.9 ± 0.41% of total PCB molar mass was observed in switchgrass treated soil, significantly higher than in unplanted soil (29.5 ± 3.4%) (p<0.05). The improved PCB removal in switchgrass treated soils could be explained by phytoextraction processes and enhanced microbial activity in the rhizosphere. Bioaugmentation with Burkholderia xenovorans LB400 was performed to further enhance aerobic PCB degradation. The presence of LB400 was associated with improved degradation of PCB 52, but not PCB 77 or PCB 153. Increased abundances of bphA (a functional gene that codes for a subunit of PCB-degrading biphenyl dioxygenase in bacteria) and its transcript were observed after bioaugmentation. The highest total PCB removal was observed in switchgrass treated soil with LB400 bioaugmentation (47.3 ± 1.22 %), and the presence of switchgrass facilitated LB400 survival in the soil. Overall, our results suggest the combined use of phytoremediation and bioaugmentation could be an efficient and sustainable strategy to eliminate recalcitrant PCB congeners and remediate PCB-contaminated soil.

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Section: Resultssupporting

confidence: 92%

Enhanced polychlorinated biphenyl removal in a switchgrass rhizosphere by bioaugmentation with Burkholderia xenovorans LB400

Liang

Meggo

et al. 2014

Ecological Engineering

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“…Biodegradation efficiency depends on the organic compounds present and soil type. Soils contaminated with PAH's are difficult to bioremediate because these hydrophobic compounds are poorly soluble and strongly sorbed to the soil matrix (Rein et al 2007). Furthermore, the root cortex is larger where plants are grown in naphthalenecontaminated sand and drought-like symptoms occur (Chapman et al 2009).…”

Section: Outcomes At Field Scalementioning

confidence: 99%

Successes and limitations of phytotechnologies at field scale: outcomes, assessment and outlook from COST Action 859

et al. 2010

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Purpose Many agricultural and brownfield soils are polluted and more have become marginalised due to the introduction of new, risk-based legislation. The European Environment Agency estimates that there are at least 250,000 polluted sites in the member states that require urgent remedial action. There is also significant volumes of wastewaters and dredged polluted sediments. Phytotechnologies potentially offer a cost-effective in situ alternative to conventional technologies for remediation of low to mediumcontaminated matrices, e.g. soils, sediments, tailings, solid wastes and waters. For further development, social and commercial acceptance, there is a clear requirement for upto-date information on successes and failures of these technologies based on evidence from the field. This review reports the outcomes from several integrated experimental attempts to address this at both field and market level in the 29 countries participating in COST Action 859. Results and discussion This review offers insight into the deployment of promising and emergent in situ phytotechnologies, for sustainable remediation and management of contaminated soils and water, that integrative research findings produced between 2004 and 2009 by members of COST Action 859. Many phytotechnologies are at the demonstration level, but relatively few have been applied in practice on large sites. They are not capable of solving all problems. Those options that may prove successful at market level are (a) phytoextraction of metals, As and Se from marginally contaminated agricultural soils, (b) phytoexclusion and phytostabilisation of metal-and AsResponsible editor: Stefan Norra M. Mench (*) UMR BIOGECO INRA 1202, Ecologie des Communautés,contaminated soils, (c) rhizodegradation of organic pollutants and (d) rhizofiltration/rhizodegradation and phytodegradation of organics in constructed wetlands. Each incidence of pollution in an environmental compartment is different and successful sustainable management requires the careful integration of all relevant factors, within the limits set by policy, social acceptance and available finances. Many plant stress factors that are not evident in short-term laboratory experiments can limit the effective deployment of phytotechnologies at field level. The current lack of knowledge on physicochemical and biological mechanisms that underpin phytoremediation, the transfer of contaminants to bioavailable fractions within the matrices, the long-term sustainability and decision support mechanisms are highlighted to identify future R&D priorities that will enable potential end-users to identify particular technologies to meet both statutory and financial requirements. Conclusions Multidisciplinary research teams and a meaningful partnership between stakeholders are primary requirements that determine long-term ecological, ecotoxicological, social and financial sustainability of phytotechnologies and to demonstrate their efficiency for the solution of large-scale pollution problems. The gap between research and develop...

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“…In addition, the transgenic strain, F113::1180, was able to metabolize Delor 103 better than initial bph donor strain, B. xenovorans LB400. Recently, another group reported higher PCB metabolization rates with transgenic P. fluorescens F113::1180 and B. xenovorans LB400, as compared to strain F113pcb (114). Using mesocosm experiments with PCB-contaminated soil, the authors reported a good survival ability of F113 strains in willow plant rhizosphere, suggesting that association of transgenic rhizosphere bacteria with plants constitute a promising approach for the treatment of PCB-contaminated soils.…”

Section: Transgenic Plant-associated Bacteria For Rhizoremediation Ofmentioning

confidence: 99%

Phytoremediation of Polychlorinated Biphenyls: New Trends and Promises

Aken

Correa

Schnoor

2009

Environ. Sci. Technol.

315

128

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Transgenic plants and associated bacteria constitute a new generation of genetically modified organisms for efficient and environmental-friendly treatment of soil and water contaminated with polychlorinated biphenyls (PCBs). This review focuses on recent advances in phytoremediation for the treatment of PCBs, including the development of transgenic plants and associated bacteria. Phytoremediation, or the use of higher plants for rehabilitation of soil and groundwater, is a promising strategy for cost-effective treatment of sites contaminated by toxic compounds, including toxic PCBs. Plants can help mitigate environmental pollution by PCBs through a range of mechanisms: besides uptake from soil (phytoextraction), plants are capable of enzymatic transformation of PCBs (phytotransformation); by releasing a variety of secondary metabolites, plants also enhance the microbial activity in the root zone, improving biodegradation of PCBs (rhizoremediation). However, because of their hydrophobicity and chemical stability, PCBs are only slowly taken up and degraded by plants and associated bacteria, resulting in incomplete treatment and potential release of toxic metabolites into the environment. Moreover, naturally occurring plant-associated bacteria may not possess the enzymatic machinery necessary for PCB degradation. In order to overcome these limitations, bacterial genes involved in the metabolism of PCBs, such as biphenyl dioxygenases, have been introduced into higher plants, following a strategy similar to the development of transgenic crops. Similarly, bacteria have then been genetically modified that exhibit improved biodegradation capabilities and are able to maintain stable relationships with plants. Transgenic plants and associated bacteria bring hope for a broader and more efficient application of phytoremediation for the treatment of PCBs.

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Degradation of PCB congeners by bacterial strains

Cited by 37 publications

References 42 publications

Enhanced polychlorinated biphenyl removal in a switchgrass rhizosphere by bioaugmentation with Burkholderia xenovorans LB400

Enhanced polychlorinated biphenyl removal in a switchgrass rhizosphere by bioaugmentation with Burkholderia xenovorans LB400

Successes and limitations of phytotechnologies at field scale: outcomes, assessment and outlook from COST Action 859

Phytoremediation of Polychlorinated Biphenyls: New Trends and Promises

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