Degradation of microcystins using immobilized microorganism isolated in an eutrophic lake

Tsuji, Kiyomi; Asakawa, Miki; Anzai, Yojiro; Sumino, Tatsuo; Harada, Kenichi

doi:10.1016/j.chemosphere.2006.02.018

Cited by 109 publications

(87 citation statements)

References 28 publications

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“…The isolation of MCs-degrading bacteria and the biodegradation of MCs under laboratory conditions have been reported before (Tsuji et al, 2006;Valeria et al, 2006;Zhang et al, 2010). These studies improve our understanding on biodegradation mechanisms and on degradation abilities of different strains (Bombach et al, 2010).…”

Section: Introductionmentioning

confidence: 69%

“…So far the number and diversity of reported MCs-degrading bacteria are still very low (Edwards et al, 2008) and the majority of them have been identified as "Sphingomonads" (Tsuji et al, 2006;Valeria et al, 2006). The reason for limited diversity is partly attributed to the failure of some bacteria to form colonies on the artificial media (Eilers et al, 2000).…”

Section: The Effect Of Natural Media On Diversification Of Mcs-degradmentioning

confidence: 99%

“…The different conditions of growth requirements between artificial media and the natural environment may lead to the degeneration of their adaptability in the environment (Markiewicz et al, 2011). Besides, the number and diversity of reported strains isolated from artificial media are still limited (Edwards et al, 2008) and the majority of them is identified as Sphingomonad (Tsuji et al, 2006;Valeria et al, 2006). In addition, potential degradative capacity, as assessed by studies performed in laboratory experimental systems, is often not fully realized in contaminated environments since field conditions are often characterized by fluctuating substrate concentrations resulting from heterogeneous source distributions (Alexander, 1985;Vroblesky and Chapelle, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced and continued degradation of microcystins using microorganisms obtained through natural media

Hong

Pan

2014

Journal of Microbiological Methods

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Microorganisms isolated through artificial media are often unsustainable in biodegrading microcystins (MCs) in natural water. Here we studied alternative approaches to isolate MCs-degrading bacteria using natural media. In comparison to two species (MS-1 and MS-2) isolated from artificial media and the failure of bacterial colonies formation using water extracts of sediment (10%, w/v), five colony species (WC-1 to WC-5) appeared using concentrated water extracts of sediment that is 10-fold enhancement of nutrient level. In the simulated biodegradation test in Lake Taihu water with continuous supply of MCs, a lag phase of 6 days was required for MS-1 and M-2 to degrade 13% and 15% of the added MC-RR and MC-LR, respectively, whereas the lag phase was only 3 days with approximately 44% and 31% removal of the added MC-RR and MC-LR by WC-1 to WC-5. During the continuous supply experiment, degradation of MCs by MS-1 and MS-2 stopped after 3 days, while degradation of MCs by WC-1 to WC-5 lasted continuously throughout the 18 day test period with 2 to 6-fold enhancement of removal rate. 16S rRNA gene sequences and phylogenetic analysis indicated the potential to amplify species of MCsdegrading bacteria when natural media were used. The results suggested that the increased adaptability of bacteria obtained through concentrated natural media was responsible for the enhanced and continued biodegradation under simulated natural water conditions.

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Section: Introductionmentioning

confidence: 69%

Section: The Effect Of Natural Media On Diversification Of Mcs-degradmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced and continued degradation of microcystins using microorganisms obtained through natural media

Hong

Pan

2014

Journal of Microbiological Methods

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“…Such filtration systems are based on undefined microorganisms collected from raw freshwater. A different pilot-scale study was performed by Bourne et al [20] and Tsuji et al [21] who proposed systems with immobilized bacteria belonging to Sphingomonas with known biodegradation activity. In the work of Bourne et al [20] an individual slow sand filter column was constructed of a sand bed with defined size and a PVC cartridge to avoid algal growth.…”

Section: Discussionmentioning

confidence: 99%

“…The documented MC degradation was 80% within 2 days. In the system proposed by Tsuji et al [21], the bacterial strain Sphingomonas sp. B-9 was immobilized on polyester in a continuous bioreactor (cell density 7.9 x 10 6 cells mL -1 ).…”

Section: Discussionmentioning

confidence: 99%