Degradation of Long-Chain Fatty Acids by Sulfate-Reducing and Methanogenic Communities

Sousa, D. Z.; Balk, Melike; Alves, M. M.; Schink, Bernhard; McInerney, Michael J.; Smidt, Hauke; Plugge, Caroline M.; Stams, Alfons Johannes Maria

doi:10.1007/978-3-540-77587-4_69

Cited by 16 publications

(15 citation statements)

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“…None of the genera present in HP culture was previously associated to LCFA FIGURE 2 | Phylogenetic tree of 16S rRNA gene sequences highlighting (in blue) the position of "Candidatus Desulfomonile palmitatoxidans" from HP culture relative to other species of the same genus, as well as selected reference sequences of SRB within the class Deltaproteobacteria. These SRB, belonging to the Desulfobacterales, Desulfarculales, and Syntrophobacterales orders, were chosen based on their capability to degrade SCFA, MCFA, and/or LCFA (Sousa et al, 2007b(Sousa et al, , 2009b(Sousa et al, , 2010. LCFA degraders are shown in bold and syntrophs are underlined.…”

Section: Resultsmentioning

confidence: 99%

Long-Chain Fatty Acids Degradation by Desulfomonile Species and Proposal of “Candidatus Desulfomonile Palmitatoxidans”

et al. 2020

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Microbial communities with the ability to convert long-chain fatty acids (LCFA) coupled to sulfate reduction can be important in the removal of these compounds from wastewater. In this work, an enrichment culture, able to oxidize the long-chain fatty acid palmitate (C16:0) coupled to sulfate reduction, was obtained from anaerobic granular sludge. Microscopic analysis of this culture, designated HP culture, revealed that it was mainly composed of one morphotype with a typical collar-like cell wall invagination, a distinct morphological feature of the Desulfomonile genus. 16S rRNA gene amplicon and metagenome-assembled genome (MAG) indeed confirmed that the abundant phylotype in HP culture belong to Desulfomonile genus [ca. 92% 16S rRNA gene sequences closely related to Desulfomonile spp.; and ca. 82% whole genome shotgun (WGS)]. Based on similar cell morphology and average nucleotide identity (ANI) (77%) between the Desulfomonile sp. in HP culture and the type strain Desulfomonile tiedjei strain DCB-1T, we propose a novel species designated as “Candidatus Desulfomonile palmitatoxidans.” This bacterium shares 94.3 and 93.6% 16S rRNA gene identity with Desulfomonile limimaris strain DCB-MT and D. tiedjei strain DCB-1T, respectively. Based on sequence abundance of Desulfomonile-morphotype in HP culture, its predominance in the microscopic observations, and presence of several genes coding for enzymes involved in LCFA degradation, the proposed species “Ca. Desulfomonile palmitatoxidans” most probably plays an important role in palmitate degradation in HP culture. Analysis of the growth of HP culture and D. tiedjei strain DCB-1T with short- (butyrate), medium- (caprylate) and long-chain fatty acids (palmitate, stearate, and oleate) showed that both cultures degraded all fatty acids coupled to sulfate reduction, except oleate that was only utilized by HP culture. In the absence of sulfate, neither HP culture, nor D. tiedjei strain DCB-1T degraded palmitate when incubated with Methanobacterium formicicum as a possible methanogenic syntrophic partner. Unlike D. tiedjei strain DCB-1T, “Ca. Desulfomonile palmitatoxidans” lacks reductive dehalogenase genes in its genome, and HP culture was not able to grow by organohalide respiration. An emended description of the genus Desulfomonile is proposed. Our study reveals an unrecognized LCFA degradation feature of the Desulfomonile genus.

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Section: Resultsmentioning

confidence: 99%

Long-Chain Fatty Acids Degradation by Desulfomonile Species and Proposal of “Candidatus Desulfomonile Palmitatoxidans”

et al. 2020

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“…The presence of SRB affiliated to Desulfovibrio genus in enrichment cultures grown on oleate or palmitate and sulfate was previously reported and linked to hydrogen consumption (Sousa et al, 2009a). Members of the Desulfovibrio genus have also been detected in oleate-degrading cultures without sulfate, although their role in these enrichments was not clear (Sousa et al, 2010;Salvador et al, 2019). Furthermore, a Desulfovibrio sp.…”

Section: Resultsmentioning

confidence: 77%

“…In the Bacteria domain, 17 sulfate-reducing species are described as LCFA-degraders. These are distributed by 16 genera and 5 different families, namely Desulfobacteraceae, Desulfarculaceae, Desulfohalobiaceae, Syntrophobacteraceae, and Peptococcaceae, within the phyla Proteobacteria and Firmicutes (Rabus et al, 2006;Sousa et al, 2009aSousa et al, , 2010. From those, 15 species can oxidize saturated LCFA (without double bonds in the aliphatic chain) up to 16 carbon atoms (C 16:0 , palmitate), and 9 of these 15 species also grow with stearate (C 18:0 ).…”

Section: Introductionmentioning

confidence: 99%

“…These two species are thus the only sulfate-reducing bacteria (SRB) described as capable of growing with oleate (C 18:1 ) or linoleate (C 18:2 ). Some SRB oxidize LCFA completely to CO 2 , while other convert LCFA to acetate, in both cases coupled to sulfate reduction to sulfide (Sousa et al, 2010). LCFA-degrading SRB are also known to grow on short-and medium-chain fatty acids (SCFA and MCFA), which contain less than 6 and 6-12 carbon atoms, respectively (Muyzer and Stams, 2008;Sousa et al, 2009a;Hao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Under sulfate-reducing conditions, SRB may also utilize hydrogen or acetate (which are intermediaries of LCFA degradation), working as syntrophic partners of LCFA-oxidizing proton-reducing acetogenic bacteria (Sousa et al, 2009b(Sousa et al, , 2010. For example, Desulfovibrio sp.…”

Section: Introductionmentioning

confidence: 99%

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