Degradation of High‐Molar‐Mass Hyaluronan by Ascorbate <i>plus</i> Cupric Ions: Effects of <scp>D</scp>‐Penicillamine Addition

Valachová, Katarína; Rapta, Peter; Kogan, Grigorij; Hrabárová, Eva; Gemeiner, Peter; Šoltés, Ladislav

doi:10.1002/cbdv.200800261

Cited by 14 publications

(4 citation statements)

References 15 publications

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“…When such a scavenging compound is loaded into the HA solution before applying the Weissbergers oxidative system, the scavenger may be tested in its function as a preventive antioxidant while, on applying the tested compound during the propagation phase of the HA degradation, its function may be tested as a chain-breaking antioxidant. The two already established experimental designs to investigate the HA degradation by the Weissbergers oxidative system [21] [22] were preferentially used on investigating both the preventive and chain-breaking antioxidative properties of the DMARD, i.e., aurothiomalate. The results represented in Fig.…”

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confidence: 99%

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Valachová

Vargová

Rapta

et al. 2011

Chemistry & Biodiversity

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The potential anti- or pro-oxidative effects of a disease-modifying antirheumatic drug, aurothiomalate, to protect high-molar-mass hyaluronan against radical degradation were investigated along with L-glutathione - tested in similar functions. Hyaluronan degradation was induced by the oxidative system Cu(II) plus ascorbate known as the Weissberger's oxidative system. The time- and dose-dependent changes of the dynamic viscosity of the hyaluronan solutions were studied by the method of rotational viscometry. Additionally, the antioxidative activity of aurothiomalate expressed as a radical-scavenging capacity based on a decolorization 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay was inspected. At the higher concentrations tested, L-glutathione showed excellent scavenging of (.) OH and peroxyl-type radicals, however, at the lowest concentration applied, its pro-oxidative effect was revealed. The effects of aurothiomalate on hyaluronan degradation were similar to that of L-glutathione, however, at the lowest concentration tested, no significant pro-oxidant effect was observed.

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confidence: 99%

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Valachová

Vargová

Rapta

et al. 2011

Chemistry & Biodiversity

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“…As detected by electron paramagnetic resonance with a spin trapper DMPO the product of the reaction of ascorbate with * OH within one hour was exclusively ascorbyl radicals, followed by a continuous increase in * OH formation. [19][20][21]…”

Section: Discussion Weissberger Biogenic Oxidation Systemmentioning

confidence: 99%

“…It is known that ascorbate has the capability to scavenge some of the forming • OH, i. e., the total concentration of 100 μM of • OH has not been achieved. As detected by electron paramagnetic resonance with a spin trapper DMPO the product of the reaction of ascorbate with • OH within one hour was exclusively ascorbyl radicals, followed by a continuous increase in • OH formation [19–21] …”

Section: Discussionmentioning

confidence: 99%

Redox Properties of Mn(III)porphyrins in Hyaluronan Oxidative Degradation: Single Electron Transfer Mechanism

Valachová,

Moura,

Šoltés

2024

Chemistry A European J

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Cationic Mn(III)‐meso‐tetraarylporphyrin derivatives, substituted in para position with different size alkyl chains, were investigated to function as antioxidants in free‐radical degradation of high‐molar‐mass hyaluronan by the methods of rotational viscometry and oximetry. The results of rotational viscometry showed that MnTM‐4‐PyP5+, MnTE‐4‐PyP5+, MnTPr‐4‐PyP5+, MnTPen‐4‐PyP5+ and MnTHep‐4‐PyP5+ showed high efficiency in decomposing H2O2, and reducing of peroxidized hyaluronan. When using oxygen electrode, MnTE‐4‐PyP5+, MnTPr‐4‐PyP5+, MnTPen‐4‐PyP5+, and MnTHep‐4‐PyP5+ applied to function as protective antioxidants in hyaluronan degradation, the uptake of dissolved oxygen from the reaction milieu was rapid, followed by continual increase in oxygen concentration up to the end of the measurement. However, when especially MnTE‐4‐PyP5+, MnTPr‐4‐PyP5+, and MnTPen‐4‐PyP5+ were examined as hyaluronan chain‐breaking antioxidants, after short‐term dissolved oxygen uptake, almost no increase in oxygen concentration was shown.

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“…It exhibits a dual behavior producing and scavenging H 2 O 2 . This fact might partially explain its pro-and antioxidative properties observed in vitro [6] [7]. Reduced l-glutathione (l-g-glutamyl-l-cysteinyl-glycine; GSH; Fig.…”

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An Alternative Standard forTrolox‐Equivalent Antioxidant‐Capacity Estimation Based on Thiol Antioxidants. Comparative 2,2′‐Azinobis[3‐ethylbenzothiazoline‐6‐sulfonic Acid] Decolorization and Rotational Viscometry Study Regarding Hyaluronan Degradation

Hrabárová

Valachová

Rapta

et al. 2010

Chemistry & Biodiversity

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Comparison of the effectiveness of antioxidant activity of three thiol compounds, D-penicillamine, reduced L-glutathione, and 1,4-dithioerythritol, expressed as a radical-scavenging capacity based on the two independent methods, namely a decolorization 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] assay and a rotational viscometry, is reported. Particular concern was focused on the testing of potential free-radical scavenging effects of thiols against hyaluronan degradation, induced by hydroxyl radicals. A promising, solvent-independent, antioxidative function of 1,4-dithioerythritol, comparable to that of a standard compound, Trolox(®), was confirmed by the 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] assay. The new potential antioxidant 1,4-dithioerythritol exhibited very good solubility in a variety of solvents (e.g., H(2)O, EtOH, and DMSO) and could be widely accepted and used as an effective antioxidant standard instead of a routinely used Trolox(®) on 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] assay.

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Degradation of High‐Molar‐Mass Hyaluronan by Ascorbate plus Cupric Ions: Effects of D‐Penicillamine Addition

Cited by 14 publications

References 15 publications

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Aurothiomalate as Preventive and Chain‐Breaking Antioxidant in Radical Degradation of High‐Molar‐Mass Hyaluronan

Redox Properties of Mn(III)porphyrins in Hyaluronan Oxidative Degradation: Single Electron Transfer Mechanism

An Alternative Standard forTrolox‐Equivalent Antioxidant‐Capacity Estimation Based on Thiol Antioxidants. Comparative 2,2′‐Azinobis[3‐ethylbenzothiazoline‐6‐sulfonic Acid] Decolorization and Rotational Viscometry Study Regarding Hyaluronan Degradation

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