Degradation of Distillery Lees (&lt;i&gt;Shochu kasu&lt;/i&gt;) by Cellulase-Producing Thraustochytrids

Taoka, Yousuke; Nagano, Naoki; Kai, Hiroyuki; Hayashi, Masahiro

doi:10.5650/jos.ess16148

Cited by 15 publications

(10 citation statements)

References 38 publications

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“…The gold nanoparticles also exhibited the maximum activity (21.84 ± 2.00 mm) against Klebsiella pneumonia (Table 6). This inhibitory activity is due to the destruction of the bacterial cell wall, as a result of hydrolytic enzymes produced by thraustochytrids [2,50]. The antibacterial activity is also attributed to fatty acids such as linoleic acid, which are proven to have antibacterial, antiviral, antitumor, anti-inflammatory, anti-proliferative and antioxidant activities [51].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Kalidasan

Nabikhan

Gomathi

et al. 2021

JMSE

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This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

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Section: Discussionmentioning

confidence: 99%

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Kalidasan

Nabikhan

Gomathi

et al. 2021

JMSE

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“…In a previous study, S. aggregatum and Schizochytrium sp. TM02Bc were cultured on distillery lees for 15 d [25]. Although the amount of the distillery lees decreased after cultivation, the growth of these strains was not investigated further.…”

Section: Screening Of Solid Food Wastes For Cultivationmentioning

confidence: 99%

“…Certain thraustochytrids are known to produce cellulase [23,24], indicating that solid-state waste might be usable as a substrate. Taoka et al (2017) [25] used distillery lees (Shochu kasu) for culturing Schizochytrium aggregatum and Schizochytrium sp. TM02Bc and found that the contents of PUFAs such as DHA and EPA were enriched in the resultant Shochu kasu mixture but no details on the growth of thraustochytrids or the PUFAs contents in the biomass were provided.…”

Section: Introductionmentioning

confidence: 99%

Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

Suenaga

Nakai

Umehara

et al. 2023

Waste Biomass Valor

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Purpose: This study aimed at valorizing solid food waste containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA).Methods: Aurantiochytrium sp. L3W that produces DHA and EPA was cultivated on eight types of solid food waste: sake lees (SL), crown daisy, Japanese mustard spinach (JMS), soy sauce residue, lemon peel (LP), orange peel, grape skin, and Hiroshimana old pickle (HOP). The biomass mixture of the remaining food waste and strain L3W was analyzed for DHA and EPA. To characterize the types of food waste, the leachability of dissolved organic carbon (DOC) and dissolved nitrogen (DN) were compared.Results: The strain L3W grew on both pasteurized and unsterilized food waste such as SL and JMS. Elution of DOC and DN from the food waste might be a factor affecting the growth of strain L3W.However, the strain L3W might utilize solid-state organic compounds in JMS. Despite the unsterile conditions, the biomass mixture of SL contained both DHA and EPA, whereas DHA was found in the biomass mixtures of JMS, LP and HOP, thereby con rming the valorization of these types of solid food waste. Unsterile mass cultivation of the strain L3W using SL and HOP in a 200 L tank also produced a biomass mixture containing 12.6 mg-DHA/g and 0.217 mg-EPA/g. These DHA and EPA contents were 1500-times and 37-times higher, respectively, than that in commercial poultry feed, indicating that these mixtures could be used as an additive in poultry feed. Statement Of NoveltyThe demand for docosahexaenoic and eicosapentaenoic acids (DHA and EPA) from sh oil has been increasing because of its bene cial use in the aquaculture and food industries but sh oil resources are now becoming unsustainable. Therefore, we have focused on using the heterotrophic microorganism, Aurantiochytrium sp. L3W, to produce DHA and EPA. The present study demonstrated that solid food waste can be used for culturing Aurantiochytrium sp. L3W without sterilization and hydrolysis pretreatments and provided evidence that the strain L3W may utilize solid-state carbon and nitrogen. We obtained biomass mixtures consisting of the remaining food waste and analyzed them for DHA and EPA. The DHA and EPA contents were 1500-times and 37-times higher, respectively, than those in commercial poultry feed. This is the rst report on valorizing solid food waste as a source of PUFAs that are potentially usable as an additive for poultry feed to produce products enriched with PUFAs, such as eggs.

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“…S1 in the supplemental material) ( 24 , 25 ). Strains belonging to Aplanochytrium , Botryochytrium , Oblongichytrium , Parietichytrium , Schizochytrium , Sicyoidochytrium , Thraustochytrium , and Ulkenia genera have been reported to produce cellulases ( 16 , 26 ). Furthermore, studies have also revealed that extracellular enzyme production is strain specific ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomic Analyses of Cellulolytic Machinery Reveal Two Nutritional Strategies of Marine Labyrinthulomycetes Protists

Liu

et al. 2023

Microbiol Spectr

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Degradation of Distillery Lees (<i>Shochu kasu</i>) by Cellulase-Producing Thraustochytrids

Cited by 15 publications

References 38 publications

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

Comparative Genomic Analyses of Cellulolytic Machinery Reveal Two Nutritional Strategies of Marine Labyrinthulomycetes Protists

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