Degradation of carbendazim and 2,4-dichlorophenoxyacetic acid by immobilized consortium on loofa sponge

Pattanasupong, Anchana; Nagase, Hirotsugu; Sugimoto, Eiko; Hori, Yasuhisa; Hirata, Kazumasa; Tani, Katsuji; Nasu, Masao; Miyamoto, Kazuhisa

doi:10.1016/s1389-1723(04)70238-8

Cited by 44 publications

(24 citation statements)

References 18 publications

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“…While ceramic discs were originally used to support acclimatized river biofilms, they may be broken during preservation and they require a large space for storage. Thus, we removed acclimatized river biofilms from ceramic discs and let them grow and attach onto loofah sponges (Pattanasupong et al 2004b). The 28-day acclimatized river biofilms on 8 ceramic discs were removed into 300 ml MSM with 50 mg l -1 methomyl, using mild sonication for 2 min.…”

Section: Acclimatizing Natural River Biofilmsmentioning

confidence: 99%

“…It has been suggested that a microbial consortium can show a higher degradation ability than a single strain because it contains both the degraders of target compounds and strains that could utilize the metabolic intermediates of the target compounds (Pattanasupong et al 2004b). This agreed with our results where acclimatized bacterial mixtures (i.e.…”

Section: Removal Of Methomyl By Isolated Bacterial Strainsmentioning

confidence: 99%

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The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Chen

Wang

et al. 2014

Int. J. Environ. Sci. Technol.

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Complex microbial communities from river biofilms might contain microorganisms capable of degrading xenobiotic pollutants such as pesticides (e.g. methomyl, which is commonly detected in rivers). Therefore, this study was used to determine the methomyl degradation potential of bacteria consortia and single bacterial strains acclimatized and isolated from natural river biofilms to provide biomaterials for bioremediation of water that is contaminated with methomyl. Natural river biofilms were culture enriched with methomyl as the sole carbon source to obtain acclimatized bacterial consortia and single bacterial strains. The microbial consortium on the ceramic discs was able to remove 91 % of added methomyl (50 mg l -1 ) in 7 days. The longer-acclimatized bacterial consortium on loofah sponges removed methomyl more quickly than the shorter-acclimatized consortium, but both had similar removal capabilities (i.e. 92.4 and 92.2 %). This finding suggested that the former might contain more methomyl degraders than the latter. However, after preservation at 25, 4 and -20°C for 1 or 3 months, the methomyl degradation ability of the bacterial consortia decreased significantly, indicating loss of methomyl degraders during preservation. Three bacterial species were isolated from acclimatized river biofilms, and only one species, identified as Sphingomonas sp., was able to remove methomyl, with a 7-day removal rate of 44.7 % when sugar was added and of 32.5 % when no sugar was added. These results suggested that an additional carbon source might slightly improve the ability of Sphingomonas sp. to degrade methomyl. Acclimatized bacterial consortia have a higher potential for treating methomyl-contaminated water than isolated bacterial species.

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Section: Acclimatizing Natural River Biofilmsmentioning

confidence: 99%

Section: Removal Of Methomyl By Isolated Bacterial Strainsmentioning

confidence: 99%

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Chen

Wang

et al. 2014

Int. J. Environ. Sci. Technol.

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“…The number of attached bacteria was analyzed after the bacteria were extracted from cumin seeds by a procedure modified from the work of Pattanasupong et al (22). The immobilized matrices were rehydrated in MS medium for 3 min, sonicated (Clifton ultrasonic bath) for 2 min, and shaken vigorously on a vortex mixer for another 2 min.…”

Section: Methodsmentioning

confidence: 99%

Cometabolic Degradation of Trichloroethene by Rhodococcus sp. Strain L4 Immobilized on Plant Materials Rich in Essential Oils

Suttinun

Müller

Luepromchai

2010

Appl Environ Microbiol

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The cometabolic degradation of trichloroethene (TCE) by Rhodococcus sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively, Rhodococcus sp. L4 was immobilized on plant materials which contain those inducers in their essential oils. Cumin seeds were the most suitable immobilizing material, and the immobilized cells tolerated up to 68 M TCE and degraded TCE continuously. The activity of immobilized cells, which had been inactivated partially during TCE degradation, could be reactivated by incubation in mineral salts medium without TCE. These findings demonstrate that immobilization of Rhodococcus sp. L4 on plant materials rich in essential oils is a promising method for efficient cometabolic degradation of TCE.Various bacteria have been reported to degrade trichloroethene (TCE) aerobically via cometabolic degradation with broad-substrate-specificity enzymes (2). However, TCE cometabolic degradation is considered an unsustainable process due to cytotoxicity, inhibition, or inactivation of TCE-degrading enzymes. These phenomena have been observed in studies using both whole cells and purified enzymes, including soluble methane monooxygenases from Methylosinus trichosporium OB3b (9) and Nitrosomonas europaea (13), toluene 2-monooxygenase from Burkholderia cepacia G4 (19, 27), toluene dioxygenase (TDO) from Pseudomonas putida F1 (15, 18), and butaneoxidizing bacteria, i.e., Pseudomonas butanovora, Mycobacterium vaccae, and Nocardioides sp. CF8 (11). Nevertheless, the addition of an inducer or growth substrate can maintain TCE cometabolic degradation. For example, the TCE-degrading activity of P. putida F1 toluene dioxygenase was restored after adding benzene, cumene, or toluene to displace TCE and its reactive intermediates from the enzyme active site (18). Arp et al. (2) suggested that the rate of enzyme maintenance and recovery depended on the extent of inactivation and the balance of TCE and inducer/growth substrate concentrations.Plant essential oils and their components, such as citral, limonene, cumene, and cumin aldehyde, have been found to induce TCE degradation in Rhodococcus sp. L4 (24). However, the removal of TCE by this bacterium was effective only for a short period. The impacts of TCE on Rhodococcus spp. and their enzymes have not been studied in detail, even though many bacteria of this genus exhibited high TCE-degrading activities (i.e., Rhodococcus erythropolis JE 77, R. erythropolis BD2, Rhodococcus sp. Sm-1, and Rhodococcus sp. Wrink) (5,6,7,16). This study therefore investigated the changes in TCE-degrading activity of Rhodococcus sp. L4 cells and TDO during exposure to TCE. Two enzyme maintenance approaches were evaluated, namely, repeated addition of essential oil components to the system and immobilization of the bacterial cells on plant material rich in essential oils. Immobilized microorganisms are generally capable of degrading ...

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“…The degradation ability of the consortium was increased by immobilization on loofa (Luffa cylindrica) sponge, in comparison with that of free-living consortium. This immobilized consortium on loofa sponge is a promising material for bioremediation of polluted water with these pesticides in paddy fields (Pattanasupong et al, 2004). Afterwards, the carbamate carbendazin was converted to 2-aminobenzimidazole by Pseudomoans isolates (Figure 10).…”

Section: Microbial Degradation Of Carbamate Pesticidesmentioning

confidence: 99%

Biodegradation of Pesticides

Porto¹,

Melgar²,

Kasemodel³

et al. 2011

Pesticides in the Modern World - Pesticides Use and Management

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Degradation of carbendazim and 2,4-dichlorophenoxyacetic acid by immobilized consortium on loofa sponge

Cited by 44 publications

References 18 publications

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Cometabolic Degradation of Trichloroethene by Rhodococcus sp. Strain L4 Immobilized on Plant Materials Rich in Essential Oils

Biodegradation of Pesticides

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