Degradation of caffeine by Pseudomonas monteilii KRM9

Abstract

Aims:The objective of this research was to isolate caffeine-degrading bacteria from coffee pulp waste in Indonesia and characterize their caffeine degradation activity. Methodology and results: The caffeine-degrading bacteria were isolated from coffee pulp wastes of Coffea arabica and C. canephora. These isolates were selected based on their caffeine degradation activity. The identification and biochemical properties of the best isolate were conducted via 16S rDNA sequence analyses and by using the Microbact kit. Meanwhile, caffeine degradation activity of this bacteria was analyzed by using LC-MS/MS. The results indicated that fourteen bacterial isolates were able to degrade caffeine. The highest caffeine degradation activity was performe d by isolate KRM9 at the rate of 99.26 ± 0.01%, on a caffeine medium after 24 h of incubation. Based on the 16S rDNA analyses, the KRM9 isolate was identified as Pseudomonas monteilii. Till present, this species has not been reported as a caffeine-degrading bacterium. However, LC-MS/MS analysis indicated that caffeine was degraded by P. monteilii KRM9 and theobromine was not the secondary metabolite of caffeine degradation. Conclusion, significance and impact of study: Pseudomonas monteilii KRM9 was detected as a new isolate of caffeine-degrading bacteria. This bacterium can be introduced as an agent to degrade caffeine from coffee pulp waste. It is expected that further research can be conducted on the overall mechanism of caffeine degradation by P. monteilii KRM9.

Cultivation and Genome Sequencing of Bacteria Isolated From the Coffee Berry Borer (Hypothenemus hampei), With Emphasis on the Role of Caffeine Degradation

“…Vega et al (2005) identified 19 bacterial genera as endophytes in coffee plants from Colombia, Hawai'i, and Mexico, with Bacillus, Burkholderia, Clavibacter, Curtobacterium, Escherichia, Micrococcus, Pantoea, Pseudomonas, Serratia, and Stenotrophomonas isolated from the seed. Pseudomonas sp., P. chloroaphis, and P. putida were identified as endophytes in various coffee plant tissues sampled (Vega et al, 2005) and P. monteilii has been isolated from coffee pulp waste (Arimurti et al, 2018). A coffee plant endophytic Pseudomonas sp.…”

Coffee Pulp: An Industrial By-product with Uses in Agriculture, Nutrition and Biotechnology

“…As for bacteria with caffeine degrading potential, a coffee pulp isolate of Pseudomonas monteilii has been studied, with a maximum degradation of 99 % at 24 h; and E. coli DH5a transformed with a plasmid isolated from Brevibacterium sp., degraded caffeine up to 2 g/L, and with a high tolerance to coffee (Nayak et al, 2012;Arimurti et al, 2018). Caffeine degrading soil bacteria have also been isolated and identified, including 5 gram-negative species, namely Pseudomonas japonica (4/12), Methylobacterium populi (5/12), Raoultella ornithinolytica (1/12), Klebsiella quasipneumoniae (1/12) and Stenotrophomonas chelatiphaga (1/12) with a potential to be used in various applications that require removal of this antinutrient (Iswanto et al, 2019b).…”

“…Chrysosporium keratinophilum Nayak et al, 2012Nayak et al, , 2013Pai et al, 2013;Arimurti et al, 2018 Gliocladium roseum…”

Characterization and Identification of Caffeine-Degrading Bacteria KAJ 36

“…These microbes can utilize caffeine as a source of carbon and nitrogen and produce CO 2 and NH 3 as the final results (Nayak et al, 2012). In the process of utilization, the concentration of caffeine decrease (Arimurti et al, 2018). There are several caffeine degrading microbes i.e.…”