Degradation of bisphenol A and acute toxicity reduction by different thermo-tolerant ascomycete strains isolated from arid soils

Mtibaà, Rim; Olicón-Hernández, Darío Rafael; Pozo, Clementina; Nasri, Monçef; Mechichi, Tahar; González, Jesús; Aranda, Elisabet

doi:10.1016/j.ecoenv.2018.02.077

Cited by 54 publications

(24 citation statements)

References 58 publications

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“…For the molecular identification, genomic DNA was extracted from fungal mycelium using DNeasy Plant Mini Kit (QIAGEN) according to the supplier's instruction. The partial sequence of the 18S ribosomal RNA gene, the internal transcribed spacer 1, the 5.8S ribosomal RNA gene, the internal transcribed spacer 2, and the 28S ribosomal RNA gene partial sequence, were amplified using universal primers ITS1/ITS4 [11]. The PCR reaction was made in a total volume of 50 µL, containing 1.25 µL of each primer (10 µM stock), 50 ng of genomic DNA, 1U of Taq DNA polymerase, 1.25 µL of the mixture dNTPs (10 mM stock), 1.5 µL of MgCl2 (25 mM stock) and 5 µL of Taq DNA buffer.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Amplified DNA was separated on 0.7% (w/v) agarose gel in TAE buffer. The amplified amplicon of around 600 bp was purified from the gel using the QIAquick® Gel Extraction Kit, sequenced using the BigDye Terminator v3.1 cycle sequencing kit and the automated ABI Prism 3730 DNA sequencer, in Secugen (Madrid, Spain), and compared with the sequences in GenBank database using the Basic Local Alignment Search Tool (BLAST) software [11].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

Mtibaà

Barriuso

Eugenio

et al. 2018

International Journal of Biological Macromolecules

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Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

Mtibaà

Barriuso

Eugenio

et al. 2018

International Journal of Biological Macromolecules

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“…However, the main limitation of the cited study was the use of commercially available end products of one of the BPA degradation pathways, while it can be biological decomposed during various mechanisms (Noszczyńska and Piotrowska-Seget 2018). Conversely, others have shown that even when organisms degrade BPA with high RE, intermediates formed during degradation are often more toxic than the parent compound (Mtibaà et al 2018). Similarly, chemical technologies such as photocatalytic oxidation did not reduce BPA toxicity but even increased it in comparison with untreated BPA solution (Plahuta et al 2014).…”

Section: Toxicity Of Medium Containing Bpamentioning

confidence: 90%

A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12

et al. 2020

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Bisphenol A (BPA) is an endocrine disrupting chemical. Its extensive use has led to the wide occurrence of BPA in various environmental ecosystems, at levels that may cause negative effects to the ecosystem and public health. Although there are many bacteria able to BPA utilization, only a few of them have a strong capacity for its biodegradation. Therefore, it is important to search for new bacteria strains, investigate their BPA biodegradation ability and potential effect of pH and other organic compounds on the process. These tasks have become the object of the present study. The results of our research show that for the newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12 after 15 days, with an initial BPA concentration of 100 mg L− 1, the highest BPA removal was achieved at pH 8, while sodium glutamate as a biostimulant best accelerated BPA degradation. Kinetic data for BPA biodegradation by both strains best fitted the Monod model. The specific degradation rate and the half saturation constant were estimated respectively as 8.75 mg L− 1 day− 1 and 111.27 mg L− 1 for Acinetobacter sp. K1MN, and 8.6 mg L− 1 day− 1 and 135.79 mg L− 1 for Pseudomonas sp. BG12. The half-maximal effective concentration (EC50) of BPA for Acinetobacter sp. K1MN was 120 mg L− 1 and for Pseudomonas sp. BG12 it was 123 mg L− 1. The toxicity bioassay (Microtox test) showed that elimination of BPA by both strains is accompanied by reduction of its toxic effect. The ability of tested strains to degrade BPA combined with their high resistance to this xenobiotic indicates that Acinetobacter sp. K1MN and Pseudomonas sp. BG12 are potential tools for BPA removal during wastewater treatment plant.

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“…The same procedure was employed for flask and bioreactor bench-scale reactions. Using the same equipment and conditions the metabolites analysis was created according to our previously reported protocol using the MassLynx software (version 4.1, Waters, Milford, USA) [21].…”

Section: Methodsmentioning

confidence: 99%

Metabolic Capability of Penicillium oxalicum to Transform High Concentrations of Anti-Inflammatory and Analgesic Drugs

et al. 2020

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Non-steroidal anti-inflammatory drugs (NSAIDs) and analgesics are two of the most employed drug groups around the world due to their use in the treatment of edema and pain. However, they also present an ecological challenge because they are considered as potential water pollutants. In this work, the biodegradation of four NSAIDs (diclofenac, ibuprofen, naproxen and ketoprofen) and one analgesic (acetaminophen) at 50 µM (initial concentration) by Penicillium oxalicum, at both flask and bioreactor bench scales, was evaluated. An important co-metabolic mechanism as part of the global bioremediation process for the elimination of these drugs was observed, as in some cases it was necessary to supplement glucose to achieve a 100% removal rate: both individually and as a complex mixture. Identical behavior in the implementation of a fluidized bench-scale batch bioreactor, inoculated with pellets of this fungus and the complex mix of the drugs, was observed. The role of the cytochrome P450 enzymes (CYP) in the biodegradation of the drugs mix were evidenced by the observation of hydroxylated by-products. The results on the reduction of toxicity (micro and phyto) were not conclusive; however, a reduction in phytotoxicity was detected.

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Degradation of bisphenol A and acute toxicity reduction by different thermo-tolerant ascomycete strains isolated from arid soils

Cited by 54 publications

References 58 publications

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

Purification and characterization of a fungal laccase from the ascomycete Thielavia sp. and its role in the decolorization of a recalcitrant dye

A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12

Metabolic Capability of Penicillium oxalicum to Transform High Concentrations of Anti-Inflammatory and Analgesic Drugs

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