Degradation of beta‐endosulfan by<i>Aspergillus Niger</i>

Mukherjee, Irani; Gopal, Madhuban

doi:10.1080/02772249409358115

Cited by 36 publications

(22 citation statements)

References 6 publications

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“…However, endodiol and endosulfan hydroxyether have been degraded by inocula from soil (27). Endosulfan strongly adsorbs to microorganisms, with the majority of the insecticide being associated with the cell membrane rather than the growth medium (22,25). Hence, degradation of endosulfan presumably leads to an accumulation of products within the cell, facilitating their further degradation.…”

Section: Enrichment Of Microorganismsmentioning

confidence: 99%

“…An essential initial step in the investigation of an enzymatic method for endosulfan detoxification is the definitive identification of a biological source of endosulfandegrading activity. Numerous studies have described the degradation of endosulfan in soils (14,26), soil inocula (11,21,27), mixed microbial cultures (1), and isolated microorganisms (9,13,17,20,22). The compound is degraded by attack at the sulfite group via both oxidation and hydrolysis to form the toxic endosulfate (endosulfan sulfate) and the nontoxic endodiol (endosulfan diol), respectively.…”

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confidence: 99%

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Enrichment of an Endosulfan-Degrading Mixed Bacterial Culture

Sutherland

Horne

Lacey

et al. 2000

Appl Environ Microbiol

155

158

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An endosulfan-degrading mixed bacterial culture was enriched from soil with a history of endosulfan exposure. Enrichment was obtained by using the insecticide as the sole source of sulfur. Chemical hydrolysis was minimized by using strongly buffered culture medium (pH 6.6), and the detergent Tween 80 was included to emulsify the insecticide, thereby increasing the amount of endosulfan in contact with the bacteria. No growth occurred in control cultures in the absence of endosulfan. Degradation of the insecticide occurred concomitant with bacterial growth. The compound was both oxidized and hydrolyzed. The oxidation reaction favored the alpha isomer and produced endosulfate, a terminal pathway product. Hydrolysis involved a novel intermediate, tentatively identified as endosulfan monoaldehyde on the basis of gas chromatography-mass spectrometry and chemical derivatization results. The accumulation and decline of metabolites suggest that the parent compound was hydrolyzed to the putative monoaldehyde, thereby releasing the sulfite moiety required for growth. The monoaldehyde was then oxidized to endosulfan hydroxyether and further metabolized to (a) polar product(s). The cytochrome P450 inhibitor, piperonyl butoxide, did not prevent endosulfan oxidation or the formation of other metabolites. These results suggest that this mixed culture is worth investigating as a source of endosulfan-hydrolyzing enzymes for use in enzymatic bioremediation of endosulfan residues.

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Section: Enrichment Of Microorganismsmentioning

confidence: 99%

mentioning

confidence: 99%

Enrichment of an Endosulfan-Degrading Mixed Bacterial Culture

Sutherland

Horne

Lacey

et al. 2000

Appl Environ Microbiol

155

158

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“…4) due to the degradation of α-isomer, as confi rmed by GC-MS. Pervious reports also indicated the formation of endosulfan sulfate as the major metabolite of endosulfan oxidation, which was not utilized by the majority of the Gram +ve bacteria [19,20] and so accumulated in the system [13,10,19]. Endosulfan sulfate has been found to be more toxic and persistent than endosulfan and might hinders the further degradation of endosulfan.…”

Section: Resultsmentioning

confidence: 99%

“…Endosulfan diol, endosulfan sulfate, endosulfan ether, endosulfan hydroxyether, endosulfan lactone and endosulfan aldehyde have been reported as the major metabolites formed during biotransformation of endosulfan. Under soil conditions, endosulfan sulfate, which is more toxic than the parent compound itself [13], has been reported to be the major metabolite and) which increases in the environment due to very low degradation rates [20].…”

Section: Introductionmentioning

confidence: 99%

Microbial biodiversity and in situ bioremediation of endosulfan contaminated soil

2008

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Molecular characterization based on 16s rDNA gene sequence analysis of bacterial colonies isolated from endosulfan contaminated soil showed the presence of Ochrobacterum sp, Burkholderia sp, Pseudomonas alcaligenes, Pseudomonas sp and Arthrobacter sp which degraded 57-90% of α-endosulfan and 74-94% of β-endosulfan after 7days. Whole cells of Pseudomonas sp and Pseudomonas alcaligenes showed 94 and 89% uptake of α-isomer and 86 and 89% of β-endosulfan respectively in 120 min. In Pseudomonas sp, endosulfan sulfate was the major metabolite detected during the degradation of α-isomer, with minor amount of endosulfan diol while in Pseudomonas alcaligenes endosulfan diol was the only product during α-endosulfan degradation. Whole cells of Pseudomonas sp also utilized 83% of endosulfan sulfate in 120 min. In situ applications of the defi ned consortium consisting of Pseudomonas alcaligenes and Pseudomonas sp (1:1) in plots contaminated with endosulfan showed that 80% of α-endosulfan and 65% of β-endosulfan was degraded after 12 weeks of incubation. Endosulfan sulfate formed during endosulfan degradation was subsequently degraded to unknown metabolites. ERIC-PCR analysis indicated 80% survival of introduced population of Pseudomonas alcaligenes and Pseudomonas sp in treated plots.

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“…These include strains of Aspergillus niger (Mukherjee and Gopal, 1994;Bhaleraoand Puranik, 2003), A. terreus, Cladosporium oxysporum (Mukherjee and Mittal, 2005), Mucor thermohyalospora (Shetty et al, 2000), Fusarium ventricosum (Siddique et al, 2003a), and Phanerochaete chrysosporium (Kullman and Matsumura, 1996).…”

Section: Open Access Research Articlementioning

confidence: 99%

Biodegradation of Organochlorine Insecticide Endosulfan by the Fungus Eutypella sp. KEF-1

Lee¹,

Park²,

Shin³

et al. 2011

Korean Journal of Environmental Agriculture

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BACKGROUD: α-and β-Endosulfan isomers of endosulfan, an endocrine disrupting chemical, are widely used cyclodiene organochlorine pesticide in worldwide, and it has widespread application in agriculture and can contaminate river-system as runoff from soil or aerial deposition METHOD AND RESULTS: In this study, an attempt was made to isolate an endosulfan degrading fungus from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture in media containing endosulfan and its metabolites as the sole carbon source, a fungus designated KEF-1 was isolated. Based on phylogenetic analysis, strain KEF-1 was assigned to the genus Eutypella. Also, the ITS (internal transcribed spacer) sequences of KEF-1 were submitted to GenBank under accession number EF581006. In potato dextrose broth containing 8 µg/mL endosulfan, strain KEF-1 completely degraded the endosulfanin 12 days. CONCLUSION:These results suggested that Eutypella sp. KEF-1 has potential as a biocatalyst for endosulfan bioremediation

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Degradation of beta‐endosulfan byAspergillus Niger

Cited by 36 publications

References 6 publications

Enrichment of an Endosulfan-Degrading Mixed Bacterial Culture

Enrichment of an Endosulfan-Degrading Mixed Bacterial Culture

Microbial biodiversity and in situ bioremediation of endosulfan contaminated soil

Biodegradation of Organochlorine Insecticide Endosulfan by the Fungus Eutypella sp. KEF-1

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