Degradation of barley glucan and lichenan by a Bacillus pumilus enzyme.

Abstract

A bacterial strain was isolated from soil, which rapidly degraded purified barley ƒÀ-glucan as well as lichenan. The strain belonged to Bacillus pumilus , and some authentic strains of this species were also shown to hydrolyze the glucan. An enzyme active on the above sub strates but not on laminaran and on CM-cellulose was partially purified from the culture fluid. This enzyme, about 27,000 in molecular weight, was found to cleave a ƒÀ-(1•¨4) linkage adjacent to a ƒÀ-(1•¨3) in the polymers. It was suggested that only an enzyme of this type should be called a 'lichenanase' and discriminated from cellulases and laminaranases.

Characterization of a Thermostable and Acidic‐Tolerable β‐Glucanase from Aerobic Fungi Trichoderma koningii ZJU‐T

“…As compared with β‐glucanase in our study, β‐glucanase from Penicillium capsulatum (Connelly and Coughlan 1991) had a T opt and T 1/2 value of 65 °C and 210 min at 65 °C, while β‐glucanases from Bacillus ploymyxa (Gosalbes and others 1991), B. pumilis (Suzuki and Kaneko 1976), and B. brevis (Louw and others 1993) had optimal temperatures of 55 to 60, 45, and 65 °C, respectively. A 75‐kDa lichenase from Clostridium thermocellum (Zverlov and others 2005) had a T opt and T 1/2 value of 78.5 °C and 30 min at 90 °C, pH opt of 6.0.…”

Draft Genome Sequence of Bacillus pumilus ku-bf1 Isolated from the Gut Contents of Wood Boring Mesomorphus sp.

“…Bacteria belonging to the genera Bacillus, Bacteroides, Butyrivibrio, Cellulosimicrobium, Citrobacter, Clostridium, Devosia, Dyadobacter, Ensifer, Kaistia, Labrys, Methanobrevibacter, Microbacterium, Ochrobactrum, Paracoccus, Pseudomonas, Rhizobium, Ruminococcus, Shinella, Siphonobacter, Stenotrophomonas, Trichonympha , and Variovorax , were found to be cellulolytic (Saxena et al, 1993 ; Schwarz, 2001 ; Gupta et al, 2012 ; Huang et al, 2012 ; Yanga et al, 2014 ). Bacillus pumilus strains are known to produce cellulase enzyme up to a maximum of 11.4 mg/g of cell dry mass (Suzuki and Kaneko, 1976 ; Kotchoni and Shonukan, 2002 ; Ariffin et al, 2006 ). The cellulase enzyme produced by B. pumilus strain EB3 has been found to be superior to fungal cellulases due to its higher optimum pH and temperature (Ariffin et al, 2006 ).…”

Mapping and Properties of bgl ( -Glucanase) Mutants of Bacillus subtilis

“…The substrate range of these bacterial P-glucan-endohydrolases is similar to lichenase (EC 3.2.1 .73 ; 1,3-1,4-P-D-glUCan 4glucanohydrolase) of germinating barley and is restricted to mixed-linked glucans like lichenan and barley P-glucan (Anderson & Stone, 1975). The purified enzymes of B. subtilis (Moscatelli et al, 1961), B. pumilus (Suzuki & Kaneko, 1976) and Bacillus sp. IMET B376 are more completely characterized.…”