Degradation of aromatic petroleum hydrocarbons by pure microbial cultures

Solanas, Anna M.; Bayona, J.M.

doi:10.1016/0045-6535(84)90196-6

Cited by 41 publications

(20 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is due to variable composition as regards alkanes, aromatic compounds, nitrogen, sulfur and oxygen-containing compounds and asphaltene fraction. For artificially-constructed consortia, the problems usually faced are inhibition between strains used and intermediate metabolites produced by some strains, which can be toxic for others (Solanas et al 1984;Rahman et al 2002). Even so, natural bacterial communities of polluted sites or oily sludges have been shown to be more effective than pure cultures and constructed consortia (Zhuang et al 2003).…”

Section: Introductionmentioning

confidence: 98%

Efficiency of refinery sludge biodegradation using municipal wastewater and activated sludge and effect of hydrocarbon concentration on culturable bacteria community

et al. 2010

View full text Add to dashboard Cite

The efficiency of activated sludge as inoculum and municipal wastewater as diluent for the biodegradation of refinery sludge was investigated. At a laboratory scale, biodegradation experiments carried out in an aerobic batch reactor showed that toxic oily sludge was biodegradable after dilution. Compared with freshwater, the use of municipal wastewater for dilution clearly enhanced the biodegradation of this toxic pollutant. Using municipal wastewater, experiments showed that, starting from an initial total hydrocarbon concentration (THC) of 750 mg L-1, we could reach a final THC of 5 mg L-1 and a COD removal of 97% in only 19 days. In addition, we found that bacterial dominance was affected by hydrocarbon load. An absolute dominance of Gram-positive strains with high hydrocarbon load (7500 mg L-1) was found. However, with a hydrocarbon load of 750 mg L-1, some Gram-negative strains were also active. This bacterial dominance also changed in the course of treatment. We conclude that municipal wastewater and activated sludge, less often used nowadays in oily sludge biodegradation processes, contained bacterial strains that are efficient and able to work in a community. Municipal wastewater is also strongly recommended as a diluent as it offers an effective low-cost solution

show abstract

Section: Introductionmentioning

confidence: 98%

Efficiency of refinery sludge biodegradation using municipal wastewater and activated sludge and effect of hydrocarbon concentration on culturable bacteria community

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Since PAHs and other components are contained within a predominantly aliphatic matrix in crude oil, most of these studies reported actions of alkane degraders on individual oil components (2,34,38,41,50). In addition to alkanes, these alkane degraders selectively depleted some alkylated PAHs (2,41), a process that has been attributed to partial oxidation due to a monooxygenase attack on the methyl groups to produce the corresponding carboxylic acids (35). Recent studies reported the isolation of a number of two-and three-ring-PAH-degrading bacterial strains from coastal sediments affected by crude oil spills.…”

mentioning

confidence: 99%

“…For example, given the resistance of 9-methyl phenanthrene to microbial oxidation in relation to the other isomers, the ratio of 3-methylphenanthrene plus 2-methylphenanthrene to 9-methylphenanthrene plus 1-methylphenanthrene has been utilized as a diagnostic ratio (47). These ratios have been defined on the basis of analysis of environmental samples (47) and results of crude oil biodegradation assays with mixed cultures (10,48) or single strains (2,41), mainly alkane-degrading pseudomonads. The actions of high-molecular-weight-PAH-degrading mycobacteria on the alkylated families of PAHs present in crude oil and derivatives have not been addressed.…”

mentioning

confidence: 99%

“…These singularities of strain AP1 relating the preference in degradation of the different alkyl-PAH families and the isomer discrimination within some of these families could be due to the types of reactions involved in the degradative attack of those compounds. The depletion of methyl derivatives of PAHs from crude oil by alkane-degrading strains (2,11,21,41) has been attributed to the mere oxidation of the alkylic carbons to form carboxylic aromatic acids (35). In this case, the accumulation of naphthalene carboxylic (III) and methylnaphthalene carboxylic (V) acids would indicate similar reactions by strain AP1, but the presence of mono (II)-and dimethylphthalates (IV) demonstrates that aromatic ring opening and further degradation of alkylated compounds also occur.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Actions of Mycobacterium sp. Strain AP1 on the Saturated- and Aromatic-Hydrocarbon Fractions of Fuel Oil in a Marine Medium

Vilà

Grifoll

2009

Appl Environ Microbiol

View full text Add to dashboard Cite

The pyrene-degrading Mycobacterium sp. strain AP1 grew in nutrient-supplemented artificial seawater with a heavy fuel oil as the sole carbon source, causing the complete removal of all linear (C 12 to C 40 ) and branched alkanes from the aliphatic fraction, as well as an extensive degradation of the three-and four-ring polycyclic aromatic hydrocarbons (PAHs) phenanthrene (95%), anthracene (80%), fluoranthene (80%), pyrene (75%), and benzo(a)anthracene (30%). Alkylated PAHs, which are more abundant in crude oils than the nonsubstituted compounds, were selectively attacked at extents that varied from more than 90% for dimethylnaphthalenes, methylphenanthrenes, methylfluorenes, and methyldibenzothiophenes to about 30% for monomethylated fluoranthenes/pyrenes and trimethylated phenanthrenes and dibenzothiophenes. Identification of key metabolites indicated the utilization of phenanthrene, pyrene, and fluoranthene by known assimilatory metabolic routes, while other components were cooxidized. Detection of mono-and dimethylated phthalic acids demonstrated ring cleavage and further oxidation of alkyl PAHs. The extensive degradation of the alkanes, the two-, three-, and four-ring PAHs, and their 1-, 2-, and 3-methyl derivatives from a complex mixture of hydrocarbons by Mycobacterium sp. strain AP1 illustrates the great substrate versatility of alkane-and PAH-degrading mycobacteria.

show abstract

“…However, most of the crude oil degradation studies reported in the literature have been carried out with single or mixed bacterial strains isolated because of their ability to grow in mineral media with crude oil as the only carbon source. [19,20]. Pseudomonas aeruginosa belongs to this group of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Combined strategy for the precipitation of heavy metals and biodegradation of petroleum in industrial wastewaters

Pérez

Cabrera

Gómez

et al. 2010

Journal of Hazardous Materials

View full text Add to dashboard Cite

a b s t r a c tThe precipitation of chromium(III), copper(II), manganese(II) and zinc(II) by biogenic hydrogen sulfide generated by sulfate-reducing bacteria, Desulfovibrio sp., and the degradation of total petroleum hydrocarbons (TPH) in the presence of heavy metal by Pseudomonas aeruginosa AT18 have been carried out. An anaerobic stirred tank reactor was used to generate hydrogen sulfide with Desulfovibrio sp. culture and the precipitation of more than 95% of each metal was achieved in 24 h (metal solutions contained: 60, 49, 50 and 80 mg L −1 of chromium, copper, manganese and zinc sulfates). A stirred tank reactor with P. aeruginosa AT18, in the presence of the heavy metal solution and 2% (v/v) of petroleum, led to the degradation of 60% of the total petroleum hydrocarbons and the removal of Cr(III) 99%, Cu(II) 93%, Zn(II) 46% and Mn(II) 88% in the medium through biosorption phenomena. These results enabled the development of an integrated system in which the two processes were combined. The overall aim of the study was achieved, with 84% of TPH degraded and all of the metals completely removed. Work is currently underway aimed at improving this system (decrease in operation time, culture of P. aeruginosa in anaerobic conditions) in an effort to apply this process in the bioremediation of natural media contaminated with heavy metals and petroleum.

show abstract

Degradation of aromatic petroleum hydrocarbons by pure microbial cultures

Cited by 41 publications

References 26 publications

Efficiency of refinery sludge biodegradation using municipal wastewater and activated sludge and effect of hydrocarbon concentration on culturable bacteria community

Efficiency of refinery sludge biodegradation using municipal wastewater and activated sludge and effect of hydrocarbon concentration on culturable bacteria community

Actions of Mycobacterium sp. Strain AP1 on the Saturated- and Aromatic-Hydrocarbon Fractions of Fuel Oil in a Marine Medium

Combined strategy for the precipitation of heavy metals and biodegradation of petroleum in industrial wastewaters

Contact Info

Product

Resources

About