Degradation of Acetonitrile by
            <i>Pseudomonas putida</i>

Nawaz, Mohamed S.; Chapatwala, Kirit D.; Wolfram, J.H.

doi:10.1128/aem.55.9.2267-2274.1989

Cited by 78 publications

(15 citation statements)

References 16 publications

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“…Both substrata were utilized at high rates by the two species of Dendryphiella . Succinamide is hydrolysed by a nitrilase, which is apparently absent from most other fungi, in contrast to succinate which is utilized by all fungi (Nawaz et al , 1989; Linardi et al , 1996). Turanose, on the other hand, serves as substrate for some but not all α‐glucosidases (Suzuki et al , 1997).…”

Section: Discussionmentioning

confidence: 99%

Carbon source utilization by the marine Dendryphiella species D. arenaria and D. salina

Cruz¹,

Schulz²,

Kubicek³

et al. 2006

FEMS Microbiology Ecology

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Carbon utilization by the marine Dendryphiella species, D. arenaria and D. salina, was investigated to detect differences in utilization and traits associated with their adaptation to the marine habitat. Fifty-four strains were isolated world-wide and tested for the utilization of various carbon sources using BIOLOG phenotype MicroArray (PM) and for the production of extracellular enzymes on solid culture media and on API ZYM assay strips. PM analysis showed that the fastest growth occurred on several monosaccharides and amino acids, 2-keto-d-gluconic acid, succinamide and turanose. Some polyols were poor carbon sources. However, the two species differed in their utilization rates of carbon sources, forming three major clusters: two separate clusters for D. arenaria and D. salina and a third cluster in which strains of the two species formed separate subclades that correlated with geographic origin. Several carbon sources were also found useful in differentiating the two speices. Dendryphiella salina did not utilize xylitol and quinic acid, whereas D. arenaria grew well on these substrates. The latter failed to grow on sorbitol and grew slowly on mannitol, both were good substrates for the former. There were also no qualitative differences between the extracellular enzymes produced, although laccase and peroxidase activities were confined only to some strains. The physiological similarities exhibited by the two species support the close relationship between D. arenaria and D. salina.

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Section: Discussionmentioning

confidence: 99%

Carbon source utilization by the marine Dendryphiella species D. arenaria and D. salina

Cruz¹,

Schulz²,

Kubicek³

et al. 2006

FEMS Microbiology Ecology

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“…Industrial use of nitrile may result in discharge of synthetic nitriles into marine, freshwater and soil environments. Increasing accumulation of such compounds in these ecosystems may cause deleterious effects since most of them are highly toxic, mutagenic and carcinogenic (7).…”

Section: Introductionmentioning

confidence: 99%

EFFECTS OF IMMOBILIZATION IN Ba-ALGINATE ON NITRILE-DEPENDENT OXYGEN UPTAKE RATES OF CANDIDA GUILLIERMONDII

Dias¹,

Rezende²,

Linardi³

2001

Braz. J. Microbiol.

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Yeast cells immobilized by entrapment in Ba-alginate gel were investigated for growth pattern and respiratory activity. The oxygen uptake rates (OUR) of cells entrapped in gels with 4% alginate were 5.2 and 23% lower than the OUR of 2% alginate and free cells, respectively. The mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for both a lower specific growth rate of immobilized cells with respect to that of free ones, and a heterogeneous biomass distribution, with progressively increasing cellular density from the inside to the outside of the matrix. Gel-matrix polymer concentration affected the maximum oxygen uptake of immobilized growing yeast cells.

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“…1) to their corresponding carboxylic acids and ammonia (3,9). Although numerous microorganisms catabolize aliphatic amides (1,3,5,10,11,13,14,19,21,22,30), acrylamide, because of its inhibitory effect on sulfhydryl proteins (4), inhibits their growth. Therefore, few microorganisms capable of degrading acrylamide have been isolated.…”

mentioning

confidence: 99%

Purification and characterization of an amidase from an acrylamide-degrading Rhodococcus sp

Nawaz

Khan

Seng

et al. 1994

Appl Environ Microbiol

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A constitutively expressed aliphatic amidase from a Rhodococcus sp. catalyzing acrylamide deamination was purified to electrophoretic homogeneity. The molecular weight of the native enzyme was estimated to be 360,000. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified preparation yielded a homogeneous protein band having an apparent molecular weight of about 44,500. The amidase had pH and temperature optima of 8.5 and 40°C, respectively, and its isoelectric point was pH 4.0. The amidase had apparent Km values of 1.2, 2.6, 3.0, 2.7, and 5.0 mM for acrylamide, acetamide, butyramide, propionamide, and isobutyramide, respectively. Inductively coupled plasma-atomic emission spectrometry analysis indicated that the enzyme contains 8 mol of iron per mol of the native enzyme. No labile sulfide was detected. The amidase activity was enhanced by, but not dependent on Fe2+, Ba2', and Cr2'. However, the enzyme activity was partially inhibited by Mg2+ and totally inhibited in the presence of Ni2+, Hg2+, Cu2+, Co2+, specific iron chelators, and thiol blocking reagents. The NH2-terminal sequence of the first 18 amino acids displayed 88% homology to the aliphatic amidase of Brevibacterium sp. strain R312.

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Degradation of Acetonitrile by Pseudomonas putida

Cited by 78 publications

References 16 publications

Carbon source utilization by the marine Dendryphiella species D. arenaria and D. salina

Carbon source utilization by the marine Dendryphiella species D. arenaria and D. salina

EFFECTS OF IMMOBILIZATION IN Ba-ALGINATE ON NITRILE-DEPENDENT OXYGEN UPTAKE RATES OF CANDIDA GUILLIERMONDII

Purification and characterization of an amidase from an acrylamide-degrading Rhodococcus sp

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