Degradation of 4-chlorophenol by the strain Rhodococcus opacus 6a

Коновалова, Е. Ю.; Solyanikova, Inna P.; Golovleva, L. A.

doi:10.1134/s0026261709060204

Cited by 16 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A7–2 [ 28 ], Alcaligenes xylosoxidans JH1 [ 29 ], Arthrobacter ureafaciens CPR706 [ 30 ], Arthrobacter chlorophenolicus A6 [ 31 ], and Herbaspirillum chlorophenolicum CPW301 (previously known as Comamonas testosteroni CPW301) [ 32 , 33 ]. The bacterial degradation of 4CP occurs via either the CC pathway [ 34 ] or the hydroquinone (HQ) pathway [ 35 ]. In the CC pathway, 4CP is first converted to 4CC by a 4CP-2-monooxygenase (EC = 1.14.13.-).…”

Section: Introductionmentioning

confidence: 99%

“…In the CC pathway, 4CP is first converted to 4CC by a 4CP-2-monooxygenase (EC = 1.14.13.-). Further degradation of 4CC then proceeds via the modified ortho -ring cleavage or meta -ring cleavage pathway [ 34 ]. In the modified ortho -cleavage pathway, 4CC is cleaved into 3-chloromuconate by a catechol-1,2-dioxygenase (EC 1.13.11.1) [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial degradation of chlorophenols and their derivatives

2014

View full text Add to dashboard Cite

Chlorophenols (CPs) and their derivatives are persistent environmental pollutants which are used in the manufacture of dyes, drugs, pesticides and other industrial products. CPs, which include monochlorophenols, polychlorophenols, chloronitrophenols, chloroaminophenols and chloromethylphenols, are highly toxic to living beings due to their carcinogenic, mutagenic and cytotoxic properties. Several physico-chemical and biological methods have been used for removal of CPs from the environment. Bacterial degradation has been considered a cost-effective and eco-friendly method of removing CPs from the environment. Several bacteria that use CPs as their sole carbon and energy sources have been isolated and characterized. Additionally, the metabolic pathways for degradation of CPs have been studied in bacteria and the genes and enzymes involved in the degradation of various CPs have been identified and characterized. This review describes the biochemical and genetic basis of the degradation of CPs and their derivatives.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacterial degradation of chlorophenols and their derivatives

2014

View full text Add to dashboard Cite

show abstract

“…According to Goodfellow et al (22), rhodococci colonies may be mucoid, rough or smooth, as well as a pigmented, yellow, orange, red, or colorless buff and cream. The splitting of a homogeneous rhodococci population into variants with different morphological, physiological, biochemical, and genotypic properties was observed by many researchers (23)(24)(25)(26).…”

Section: Discussionmentioning

confidence: 97%

Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60

Postolachi

Rastimesina

Josan

2021

OH&RM

View full text Add to dashboard Cite

Introduction. In recent years, due to wide applications of nanotechnologies in various fields, the safety of nanomaterials has become a pressing issue. Fullerene C60 is not an exception. Research on the activity of microorganisms and their interaction with nanoparticles is of major importance, both for microorganisms and for the ecosystem as a whole. Material and methods. Fullerene C60 powder was purchased from Sigma-Aldrich. The object of study was R. rhodochrous CNMN-Ac-05 strain. The number of viable bacterial cells was estimated by colony-forming units (CFU). The morphological features of the rhodococci colonies have been described according to the usual microbiological method. Results. It was established that fullerene C60 in concentrations of 1-25 mg/L fullerene C60 stimulated the growth of R. rhodochrous by 2.4-2.8 times. As the concentration of fullerene C60 increased up to 50-100 mg/L, the multiplication and growth of rhodococci decreased by 29.5% and 38% respectively. In the presence of 1-10 mg/L fullerene C60 the rhodococci population remained homogeneous, being composed of 100% S type colonies. The increase of fullerene C60 concentration led both to the decrease in the CFU number and to the appearance of R type colonies, up to 1.3% of population. Conclusions. Fullerene C60 in concentrations 1-100 mg/L had no obvious toxic effect on the rhodococci strain. The optimum concentration is 10 mg/L. The concentrations higher than 25 mg/L led to the dissociation of rhodococcal population and diminution in the CFU counts, but not to the total inhibition.

show abstract

“…The splitting of a homogeneous rhodococci population into variants with different mor pho logical, physiological, biochemical, and genotypic properties have been observed by many researchers. For example R. opacus 1CP strain grown on chlorophenols has dissociated on two morphologically and physiologically distinct variants and a labile transitional variant, which was an intermediate case [19,27]. Studying of the ability of actinobacteria R. opacus 1CP to survive under unfavorable conditions (starvation, oxidative stress, low temperature (4 °С), and dehydration) has revealed that general strategy for survival was decreased cell size/volume and formation of densely-packed cell conglomerates [20].…”

Section: F I G 1 Influence Of Magnetite Nps On the Viability Of R Rhodochrous Cnmn-ac-05 Strainmentioning

confidence: 99%

Viability and Colony Morphology Variation of Rhodococcus rhodochrous CNMN-Ac-05 in the Presence of Magnetite Nanoparticles

Postolachi¹,

Rastimesina²,

Josan³

et al. 2021

Mikrobiol. Z.

View full text Add to dashboard Cite

In recent decades the use of nanotechnologies in the remediation of xenobiotic substances has proven its effectiveness, but not its safety. Nanoparticles often accumulate in the remedied environment, having, over time, toxic effects on living organisms. In this context, research on the vital activity of microorganisms and their interaction with nanoparticles is of major importance. Aim of the research was to determine the influence of Fe3O4 nanoparticles, obtained by different ways (laboratory method and synthesis in the reactor) on the viability and colony morphology of Rhodococcus rhodochrous CNMN-Ac-05 strain. Methods. Encapsulated magnetite (Fe3O4) nanoparticles were synthesized by chemical co-precipitation method, using iron(II) sulfate and iron(III) chloride in the presence of poly-N-vinylpyrrolidone, used as a stabilizer. Fe3O4 SR (Synthesis in the Reactor) was produced in the multifunctional reactor VGR-50, at the same conditions. Cell biomass was determined on the spectrophotometer by the optical density at 540 nm,with subsequent recalculation to cell dry weight according to the calibration curve. The cell dry weight was determined by gravimetric method. The morphological features of the rhodococci colonies were described according to the standard microbiological method. Results. It was established that magnetite nanoparticles in concentrations of 1–100 mg/L were not toxic to the R. rhodochrous strain, had a positive effect on the viability of rhodococci by stimulating the growth of biomass, regardless of their concentration and the method of their synthesis. In the presence of Fe3O4 nanoparticles the population dissociated to S1, S2, R1, R2 forms, and S-R type of colonies, while the basic morphological features of R. rhodochrous colonies corresponded to type S1. Conclusions. The optimal concentration of magnetite nanoparticles, which stimulated the growth and development of R. rhodochrous was 25 mg/L for Fe3O4 and 50 mg/L Fe3O4 SR. At all concentration of Fe3O4 nanoparticles the main colony morphotype of the rhodococci was smooth S1-type; the new types of colonies represented only 0.1–0.6% of the population, and the lowest degree of variability corresponded with the highest colony-forming units index.

show abstract

Degradation of 4-chlorophenol by the strain Rhodococcus opacus 6a

Cited by 16 publications

References 2 publications

Bacterial degradation of chlorophenols and their derivatives

Bacterial degradation of chlorophenols and their derivatives

Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60

Viability and Colony Morphology Variation of Rhodococcus rhodochrous CNMN-Ac-05 in the Presence of Magnetite Nanoparticles

Contact Info

Product

Resources

About