Degradation of 2,4-dichlorophenol catalyzed by the immobilized laccase with the carrier of Fe3O4@MSS–NH2

Huang, Yan; Xi, Yanjie; Yang, Yuxiang; Cheng, Chen; Yuan, Hongming; Liu, Xiangnong

doi:10.1007/s11434-013-0086-4

Cited by 26 publications

(4 citation statements)

References 13 publications

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“…In a slightly high pH environment, the dissociation equilibrium of 2,4-DCP shifted in the direction of producing more H + . According to the principle of chemical reaction equilibrium, anions (OH − ) enable and promote the catalytic oxidation reaction [ 56 ]. However, in this study, this led to lower catalytic oxidation efficiency when the pH value was further increased.…”

Section: Resultsmentioning

confidence: 99%

Mesoporous Polymeric Ionic Liquid via Confined Polymerization for Laccase Immobilization towards Efficient Degradation of Phenolic Pollutants

et al. 2023

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Laccase immobilization is a promising method that can be used for the recyclable treatment of refractory phenolic pollutants (e.g., chlorophenols) under mild conditions, but the method is still hindered by the trade-off limits of supports in terms of their high specific surface area and rich functional groups. Herein, confined polymerization was applied to create abundant amino-functionalized polymeric ionic liquids (PILs) featuring a highly specific surface area and mesoporous structure for chemically immobilizing laccase. Benefiting from this strategy, the specific surface area of the as-synthesized PILs was significantly increased by 60-fold, from 5 to 302 m2/g. Further, a maximum activity recovery of 82% towards laccase was recorded. The tolerance and circulation of the immobilized laccase under harsh operating conditions were significantly improved, and the immobilized laccase retained more than 84% of its initial activity after 15 days. After 10 cycles, the immobilized laccase was still able to maintain 80% of its activity. Compared with the free laccase, the immobilized laccase exhibited enhanced stability in the biodegradation of 2,4-dichlorophenol (2,4-DCP), recording around 80% (seven cycles) efficiency. It is proposed that the synergistic effect between PILs and laccase plays an important role in the enhancement of stability and activity in phenolic pollutant degradation. This work provides a strategy for the development of synthetic methods for PILs and the improvement of immobilized laccase stability.

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Section: Resultsmentioning

confidence: 99%

Mesoporous Polymeric Ionic Liquid via Confined Polymerization for Laccase Immobilization towards Efficient Degradation of Phenolic Pollutants

et al. 2023

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“…Residual 2,4-DCP was measured through spectrophotometry using 4-aminoantipyrine solution and ferricyanide solution according to the method mentioned by Yang and Humphery [21] and Huang et al [22]. The resulting color was measured at 510 nm, and the concentration of residual 2,4-DCP was calculated from the standard curve of different 2,4-DCP concentrations ranging from 10 to 100 mg/ml.…”

Section: Determination Of Residual 24-dcpmentioning

confidence: 99%

2,4-Dichlorophenol biotransformation using immobilized marine halophilic Bacillus subtilis culture and laccase enzyme: application in wastewater treatment

Farag

El-Naggar

Ghanem

2022

Journal of Genetic Engineering and Biotechnology

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Background 2,4-Dichlorophenol (2,4-DCP) is a very toxic aromatic compound for humans and the environment and is highly resistant to degradation. Therefore, it is necessary to develop efficient remediation and cost-effective approaches to this pollutant. Microbial enzymes such as laccases can degrade phenols, but limited information is known about immobilized bacterial laccase and their reuse. Methods Immobilization of marine halophilic Bacillus subtilis AAK cultures via entrapment and adsorption techniques and degradation of different phenolic compounds by immobilized cells were estimated. Partial purification and immobilization of laccase enzymes were carried out. In addition, the biodegradation of 2,4-DCP and others contaminated by wastewater was investigated. Results Immobilization of cells and partially purified laccase enzymes by adsorption into 3% alginate increased 2,4-DCP biotransformation compared with free cells and free enzymes. In addition, the reuse of both the immobilized culture and laccase enzymes was evaluated. The highest removal of 2,4-DCP from pulp and paper wastewater samples inoculated by immobilized cells and the immobilized enzyme was 90% and 95%, respectively, at 50 h and 52 h of incubation, compared to free cells and free enzyme. Conclusion The results of this study have revealed the immobilization of a biocatalyst and its laccase enzyme as a promising technique for enhancing the degradation of 2,4-DCP and other toxic phenolic and aromatic compounds. The reuse of the biocatalyst and its laccase enzyme enabled the application of this cost-effective bioremediation strategy.

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“…Under optimal conditions, the removal rate of 2,4-DCP reached 88%. After ve cycles of operation, the removal rate was 61.5% (Huang 2014). In another study, we used MNPs@ALG@SiO 2 composites (ALG: alginic acid) with a magnetic host-guest cage structure to embed a speci c enzyme as its guest in the host matrix.…”

Section: Introductionmentioning

confidence: 99%

Pyrite-assisted degradation of methoxychlor by laccase immobilized on Fe3S4/earthworm-like mesoporous SiO2

Yang,

Chang

et al. 2024

Environ Sci Pollut Res

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Laccase immobilized and cross-linked on Fe 3 S 4 /earthworm-like mesoporous SiO 2 (Fe 3 S 4 /EW-mSiO 2 ), was used to degrade methoxychlor (MXC) in aqueous environments. The effects of various parameters on the degradation of MXC were determined using free and immobilized laccase. Immobilization improved the thermal stability and reuse of laccase signi cantly. Under the conditions of pH 4.5, temperature 40°C and reaction time 8h, the degradation rate of MXC by immobilized laccase reached a maximum value of 40.99% and remained at 1/3 of the original after six cycles. The excellent degradation performance of Fe 3 S 4 /EW-mSiO 2 was attributable to the pyrite (FeS 2 ) impurity in Fe 3 S 4 , which could act as an electron donor in reductive dehalogenation. Sul de groups and Fe 2+ reduced the activation energy of the system resulting in pyrite-assisted degradation of MXC. The degradation mechanism of MXC in aqueous environments by laccase immobilized on Fe 3 S 4 /EW-mSiO 2 was determined via mass spectroscopy of the degradation products. This study is a new attempt to use pyrite to support immobilized laccase degradation. mol − 1 , which is attributed to the fact that pyrite, FeS 2 , in Fe 3 S 4 can rapidly catalyze a reductive dehalogenation reaction, thus accelerating the degradation reaction of MXC. Moreover, 1-cyclohexyl-1phenylethanol and 3-methyl-2-heptanol were identi ed as the degradation products using gas chromatography/mass spectroscopy, and the degradation mechanism of MXC was accordingly inferred.The information provided by this work will undoubtedly facilitate the study of the control of chlorinated organic pesticides.

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Degradation of 2,4-dichlorophenol catalyzed by the immobilized laccase with the carrier of Fe3O4@MSS–NH2

Cited by 26 publications

References 13 publications

Mesoporous Polymeric Ionic Liquid via Confined Polymerization for Laccase Immobilization towards Efficient Degradation of Phenolic Pollutants

Mesoporous Polymeric Ionic Liquid via Confined Polymerization for Laccase Immobilization towards Efficient Degradation of Phenolic Pollutants

2,4-Dichlorophenol biotransformation using immobilized marine halophilic Bacillus subtilis culture and laccase enzyme: application in wastewater treatment

Pyrite-assisted degradation of methoxychlor by laccase immobilized on Fe3S4/earthworm-like mesoporous SiO2

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