Degradation of 2,4-D herbicide by microorganisms isolated from Brazilian contaminated soil

Silva, Tatiane M.; Stets, Maria Isabel; Mazzetto, André Mancebo; Andrade, Fabiana Diuk; Pileggi, Sônia Alvim Veiga; Fávero, Paulo R.; Cantú, Marcelo Delmar; Carrilho, Emanuel; Carneiro, Paulo Irajara Borba; Pileggi, Marcos

doi:10.1590/s1517-83822007000300026

Cited by 61 publications

(22 citation statements)

References 17 publications

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“…In the growth curve no lag phase was observed, demonstrating the ability of strains to rapidly adapt to the culture medium. Similar phenomena were observed by Silva et al [29], who studied the potential to degrade 2,4-D among 25 strains isolated from soil regularly treated with herbicide for three years. As a consequence of microbial growth and 2,4-D metabolism, a decrease of medium pH was measured.…”

Section: Degradation Of 24-d and 24-dcp In Mineral Salt Mediumsupporting

confidence: 85%

Biodegradation kinetics of 2,4-D by bacterial strains isolated from soil

2011

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The aim of the study was to characterize the 2,4-dichlorophenoxyacetic acid (2,4-D) degradative potential of three bacterial strains identified by MIDI-FAME profiling as Burkholderia cepacia (DS-1), Pseudomonas sp. (DS-2) and Sphingomonas paucimobilis (DS-3) isolated from soil with herbicide treatment history. All strains were capable of using herbicide as the only source of carbon and energy when grown in mineral salt medium (MSM) containing 2,4-D (50 mg/l). Over a 10 day incubation period, 69%, 73% and 54% of the initial dose of 2,4-D were degraded by strains DS-1, DS-2 and DS-3, respectively. Analysis of 2,4-dichlorophenol (2,4-DCP) concentration, the main metabolite of 2,4-D degradation, revealed that strains DS-1 and DS-2 may also have the potential to metabolize this compound. The percentage of 2,4-DCP removal was 67% and 77% in relation to maximum values of 9.5 and 9.2 mg/l determined after 4 and 2 days for MSM+DS-1 and MSM+DS-2, respectively. The degradation kinetics of 2,4-D (50 mg/kg) in sterile soil (SS) showed different potential of tested strains to degrade 2,4-D. The times within which the initial 2,4-D concentration was reduced by 50% (DT50) were 6.3, 5.0 and 9.4 days for SS+DS-1, SS+DS-2 and SS+DS-3, respectively.

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Section: Degradation Of 24-d and 24-dcp In Mineral Salt Mediumsupporting

confidence: 85%

Biodegradation kinetics of 2,4-D by bacterial strains isolated from soil

2011

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“…Studies exist about the influence of isolated microorganisms on the degradation of other organic compounds (5,18,23,32,33,35).…”

Section: Discussionmentioning

confidence: 99%

Microbial degradation of sulfentrazone in a Brazilian rhodic hapludox soil

Martínez¹,

Silva²,

Fay³

et al. 2010

Braz. J. Microbiol.

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Sulfentrazone is amongst the most widely used herbicides for treating the main crops in the State of São Paulo, Brazil, but few studies are available on the biotransformation of this compound in Brazilian soils.Soil samples of Rhodic Hapludox soil were supplemented with sulfentrazone (0.7 µg active ingredient (a.i.) g -1 soil) and maintained at 27ºC. The soil moisture content was corrected to 30, 70 or 100 % water holding capacity (WHC) and maintained constant until the end of the experimental period. Herbicide-free soil samples were used as controls. Another experiment was carried out using soil samples maintained at a constant moisture content of 70% WHC, supplemented or otherwise with the herbicide, and submitted to different temperatures of 15, 30 and 40º C. In both experiments, aliquots were removed after various incubation periods for the quantitative analysis of sulfentrazone residues by gas chromatography.Herbicide-degrading microorganisms were isolated and identified. After 120 days a significant effect on herbicide degradation was observed for the factor of temperature, degradation being higher at 30 and 40º C. A half-life of 91.6 days was estimated at 27º C and 70 % WHC. The soil moisture content did not significantly affect sulfentrazone degradation and the microorganisms identified as potential sulfentrazone degraders were Nocardia brasiliensis and Penicillium sp. The present study enhanced the prospects for future studies on the bio-prospecting for microbial populations related to the degradation of sulfentrazone, and may also contribute to the development of strategies for the bioremediation of sulfentrazone-polluted soils.

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“…Among these herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D) is very difficult to be decomposed due to its chemical and biological stability [3,4]. It is considered to be a carcinogenic and high toxic pollutant, injuring human and animals [5].…”

Section: Introductionmentioning

confidence: 99%