Degradation-Dependent Controlled Delivery of Doxorubicin by Glyoxal Cross-Linked Magnetic and Porous Chitosan Microspheres

Abstract

Glyoxal cross-linked porous magnetic chitosan microspheres, GMS (∼170 μm size), with a tunable degradation profile were synthesized by a water-in-oil emulsion technique to accomplish controlled delivery of doxorubicin (DOX), a chemotherapeutic drug, to ensure prolonged chemotherapeutic effects. The GMS exhibit superparamagnetism with saturation magnetization, M s = 7.2 emu g –1 . The in vitro swelling and degradation results demonstrate that a swelling plateau of GMS is reached at 24 h, while degradation can be modulated to begin at 96–120 h by formulating the cross-linked network using glyoxal. MTT assay, live/dead staining, and F-actin staining (actin/DAPI) validated the cytocompatibility of GMS, which further assured good drug loading capacity (35.8%). The release mechanism has two stages, initiated by diffusion-inspired release of DOX through the swollen polymer network (72 h), which is followed by a disintegration-tuned release profile (>96 h) conferring GMS a potential candidate for DOX delivery.

Bioadhesives with Antimicrobial Properties

“…Antimicrobial agents are either physically entrapped within the bioadhesive and released via diffusion, or chemically bound to bioadhesive matrix and their release is stimulus- [74,75] or degradation-dependent. [76,77] Examples of bioadhesives incorporating antimicrobial agents include antimicrobial peptide Tet213 conjugated to methacryloyl-substituted recombinant human tropoelastin (MeTro)/GelMA, [63] and ciprofloxacin physically entrapped in dendronized polyvinylpyrrolidone (PVP)chitosan film (Figure 2A). [78] Nanocomposites are more complicated in design and optimization; however, they have unique features.…”

Advances in Biomedical and Environmental Applications of Magnetic Hydrogels

“…76 developed a salecan-based magnetic hydrogel for drug release testing of doxorubicin hydrochloride (DOX). Salecan is a water-soluble extracellular glucan that has been copolymerized with vinylacetic acid (VA) and hydroxyethyl acrylate (HEA) in the presence of Fe 3 O 4 @ DOX pH/temperature/magnetic field-responsive release 91 DOX pH/temperature-responsive release magnetic chitosan nanocomposite 92 DOX pH-responsive release magnetic dextran microgels 80 DOX pH/magnetic fieldresponsive MNP@DAS@CPE 93 DOX pH-responsive release Fe 3 O 4 /P(NIPAM-AA-MAPEG) nanogels 94 DOX pH-responsive release glyoxal cross-linked porous magnetic chitosan microspheres 95 DOX pH-responsive release CS/PAA/Fe 3 O 4 nanocomposite 86 5-Fu pH-responsive release Fe 3 O 4 -Dex-MA-g P(NVI/NVCL) 96 5-Fu pH/temperature-responsive ferrogel (methacrylic acid or 2-aminoethyl methacrylate hydrochloride containing ferronanoparticles) 66 5-Fu pH/magnetic fieldresponsive release magnetic gelatin microspheres (MGMs) 20 5-Fu magnetic field-responsive MC-PCL magnetic hydrogel 97 5-Fu pH/magnetic fieldresponsive release CMCS-Fe 3 O 4 microspheres 98 5-Fu magnetic field-responsive release magnetic alginate/chitosan microspheres (DM-ACMSs) 62 DOX magnetic field-responsive release MPTMS-MNP-poly(GMA-g-MACD) 99 5-Fu pH-responsive release agarose nanoparticles and is suitable for hydrogel formation. At pH 4.5, cumulative DOX release reached 76% after 64 h, which was significantly higher than the 16.9 and 10.5% observed at pH 6.8 and 7.4, respectively.…”

A tetracycline hydrochloride-loaded SiO2/polycaprolactone composite from bamboo stem for controlled drug release study

“…During the incubation period, the PBS solution was withdrawn at pre-defined time intervals to measure the absorbance with a UV/visible spectrophotometer (T70+ UV/Vis Spectrometer, PG Instruments Ltd.) at a wavelength of 358 nm to determine the concentration of TCH released from the composite, in accordance with previous studies. 21,22 The TCH stock concentration of 5−100 µg/mL in PBS solution was initially used to establish the calibration curve from which the drug concentration was extrapolated using an absorbance-concentration calibration curve. The cumulative TCH released from the SiPCL/TCH composite was computed relative to…”