Degradation characteristics and metabolic pathway of 17β-estradiol (E2) by Rhodococcus sp. DS201

Yu, Qingmiao; Wang, Ping; Liu, Dongbo; Gao, Ruixia; Shao, Huanhuan; Zhao, Hongyan; Ma, Zhe; Wang, Dan; Huo, Hongliang

doi:10.1007/s12257-016-0283-5

Cited by 25 publications

(18 citation statements)

References 19 publications

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“…The degradation steps follows release of HCOOH or CO 2 and hydroxylation of C-9 occurs producing keto group. Further, the B ring is reported to cleave followed by breakdown of D ring [1,32]. The GC MS analysis in this study shows that parent compound, E2 was degraded to glucose in the final stage.…”

Section: In Vivo Biodegradation Of 17 β-Estradiol By Trichoderma Citrmentioning

confidence: 60%

Mycoremediation of 17 β‐Estradiol Using Trichoderma citrinoviride Strain AJAC3 along with Enzyme Studies

Chatterjee

Abraham

2019

Env Prog and Sustain Energy

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Extensive and inappropriate use of steroids in drugs and pharmaceutical products and improper release of steroids into the immediate environment has found to affect soil and water resulting in imbalance of ecosystem. The steroids reach environment due to inaccurate disposal of expired drugs as well as manufacturing disposal from pharmaceutical industries and are urgently required to be removed from the environment. The present study deals with elimination of 17 β‐estradiol (E2) using Trichoderma citrinoviride AJAC3. The fungal isolate could tolerate E2 and degrade 99.6% of the contaminant at concentration of 200 mg L−1. Trichoderma citrinoviride AJAC3 secreted extracellular ligninolytic enzymes which played significant role in degradation of E2. High Performance Liquid Chromatography (HPLC) was used to determine the biodegradation pattern of E2 and confirmed by Gas Chromatography Mass Spectroscopy (GC–MS), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Kinetics study revealed that the degradation pathway followed first‐order kinetic model. The results affirmed that the isolate was able to biodegrade E2 efficiently. © 2019 American Institute of Chemical Engineers Environ Prog, 38:e13142, 2019

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Section: In Vivo Biodegradation Of 17 β-Estradiol By Trichoderma Citrmentioning

confidence: 60%

Mycoremediation of 17 β‐Estradiol Using Trichoderma citrinoviride Strain AJAC3 along with Enzyme Studies

Chatterjee

Abraham

2019

Env Prog and Sustain Energy

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“…In the B pathway, 4-OH-E1 is cleaved between C-4 and C-5, leading to its microbial-mediated oxidation and the formation of substances described in Yu et al [27]. The C-5 position is then oxidized to a carboxyl group through a series of redox reactions, resulting in the formation of intermediate metabolite i [34], a substance that was among the degradation products for the RS strain and the mixed culture.…”

Section: Analysis Of the Mechanisms Of E2 Degradation By The Mixed Bamentioning

confidence: 98%

“…Thus, E2 degradation is more thorough by degradation of mixed cultures than that observed in the single-strain cultures, and greater degradation ability was exhibited in the mixed culture system than in the single culture systems. Bacterial competition can be avoided by the existence of metabolic diversity among bacteria that degrade natural oestrogen [27], as the strains are competing not only for substrates but also for metabolic intermediates.…”

Section: Analysis Of the E2 Degradation Productsmentioning

confidence: 99%

“…In the E2 degradation experiment, we analyzed the E2 metabolites in the mixed culture according to their molecular formulae. The degradation of E2 has been shown to typically start from E1 under anaerobic, anoxic and aerobic conditions [7,27]. In the E2 degradation pathway for the mixed culture, E1 was formed by the hydroxylation of E2 at the c-17 position prior to further degradation of E1.…”

Section: Analysis Of the Mechanisms Of E2 Degradation By The Mixed Bamentioning

confidence: 99%

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Degradation of 17β-estradiol and products by a mixed culture of Rhodococcus equi DSSKP-R-001 and Comamonas testosteroni QYY20150409

Wang

Shao

Zhu

et al. 2019

Biotechnology & Biotechnological Equipment

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In this study, we investigated environmental oestrogen 17b-estradiol (E2) degradation by a mixed culture of Rhodococcus equi DSSKP-R-001(RS) and Comamonas testosteroni QYY20150409(CT). The results demonstrated that the two strains exhibited synergistic E2 degradation activity, showing that multispecies bacterial systems can improve microbial survival and activity. The E2 degradation rate for the mixed culture system was 94% under conditions in which E2 was the sole source of carbon and growth energy, while the degradation rates of the R. equi DSSKP-R-001 and C. testosteroni QYY20150409 single strains were 86% and 76%, respectively. Comparing and analyzing the degradation products of E2, intermediate cross-metabolism products were observed in the culture containing the two bacteria. The metabolic products of benzene were essentially removed during the process of E2 degradation in the mixed culture, which showed stronger degradation ability than cultures of the individual strains. The results from this investigation on the degradation of E2 by individual and mixed bacterial cultures provide a theoretical basis for the microbial remediation of environmental oestrogens.

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“…The complete mineralization of estrogens to CO 2 can be accomplished by a limited number of bacteria, mainly from the phyla Proteobacteria and Actinobacteria (Fujii et al, 2002;Fahrbach et al, 2006;Yu et al, 2007Yu et al, , 2016Kurisu et al, 2010;Chen et al, 2017;Wang et al, 2019;Li et al, 2020). Although some biotransformation steps have been described in estrogen-degrading strains, the complete degradation pathway still remains unknown (Yu et al, 2013;Wang et al, 2014Wang et al, , 2018Wang et al, , 2019Chen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725

et al. 2020

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We have analyzed the catabolism of estrogens in Novosphingobium tardaugens NBRC 16725, which is able to use endocrine disruptors such as 17β-estradiol, estrone, and estriol as sole carbon and energy sources. A transcriptomic analysis enabled the identification of a cluster of catabolic genes (edc cluster) organized in two divergent operons that are involved in estrogen degradation. We have developed genetic tools for this estrogen-degrading bacterium, allowing us to delete by site-directed mutagenesis some of the genes of the edc cluster and complement them by using expression plasmids to better characterize their precise role in the estrogen catabolism. Based on these results, a catabolic pathway is proposed. The first enzyme of the pathway (17β-hydroxysteroid dehydrogenase) used to transform 17β-estradiol into estrone is encoded out of the cluster. A CYP450 encoded by the edcA gene performs the second metabolic step, i.e., the 4-hydroxylation of estrone in this strain. The edcB gene encodes a 4-hydroxyestrone-4,5-dioxygenase that opens ring A after 4-hydroxylation. The initial steps of the catabolism of estrogens and cholate proceed through different pathways. However, the degradation of estrogens converges with the degradation of testosterone in the final steps of the lower catabolic pathway used to degrade the common intermediate 3aα-H-4α(3′-propanoate)7a-β-methylhexahydro-1,5-indanedione (HIP). The TonB-dependent receptor protein EdcT appears to be involved in estrogen uptake, being the first time that this kind of proteins has been involved in steroid transport.

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Degradation characteristics and metabolic pathway of 17β-estradiol (E2) by Rhodococcus sp. DS201

Cited by 25 publications

References 19 publications

Mycoremediation of 17 β‐Estradiol Using Trichoderma citrinoviride Strain AJAC3 along with Enzyme Studies

Mycoremediation of 17 β‐Estradiol Using Trichoderma citrinoviride Strain AJAC3 along with Enzyme Studies

Degradation of 17β-estradiol and products by a mixed culture of Rhodococcus equi DSSKP-R-001 and Comamonas testosteroni QYY20150409

Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725

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