Degradable polyethylenimine-alt-poly(ethylene glycol) copolymers as novel gene carriers

Park, Mi Ran; Han, Kyoung-Sik; Han, In K.; Cho, Myung‐Haing; Nah, Jae Woon; Choi, Yun Jaie; Cho, Chong Su

doi:10.1016/j.jconrel.2005.04.008

Cited by 225 publications

(171 citation statements)

References 44 publications

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“…The low efficiency of conventional therapies in achieving long-term survival of patients with lung cancer calls for 22 To overcome such obstacles, numerous attempts have been made for the modification of existing carriers. In the previous works, we reported that modified UAC-DNA complex enhanced gene delivery activity and aerosol gene delivery with UAC/PDCD4 was compatible with in vivo gene delivery and could be applied as a noninvasive gene therapy.…”

Section: Discussionmentioning

confidence: 99%

Urocanic acid-modified chitosan-mediated PTEN delivery via aerosol suppressed lung tumorigenesis in K-rasLA1 mice

Jin

et al. 2008

Cancer Gene Ther

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The low efficiency of conventional therapies in achieving long-term survival of lung cancer patients calls for development of novel options. Revisiting of aerosol gene delivery may provide an alternative for safe and effective treatment for lung cancer. In this study, imidazole ring-containing urocanic acid-modified chitosan (UAC) designed in the previous study was used as a gene carrier. The potential effects of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) on Akt-related signals and cell cycle regulation were evaluated. Aerosols of UAC-PTEN were delivered into K-ras LA1 lung cancer model mice through the nose-only inhalation system twice a week for total 4 weeks. Delivered PTEN suppressed lung tumor development significantly through nuclear complex formation between PTEN and p53, suppressing Akt-related signals as well as cell cycle regulation. Together, our results suggest that aerosol delivery of UAC-PTEN may be compatible with noninvasive in vivo gene therapy.

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Section: Discussionmentioning

confidence: 99%

Urocanic acid-modified chitosan-mediated PTEN delivery via aerosol suppressed lung tumorigenesis in K-rasLA1 mice

Jin

et al. 2008

Cancer Gene Ther

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“…Nonviral vectors manifest considerable advantages over their viral counterparts. However, nonviral vector systems have their own down sides, such as low transfection efficiency, short duration of target gene expression, and limited targeting (23). To overcome such obstacles, numerous attempts have been made for modifications of existing carriers.…”

Section: Discussionmentioning

confidence: 99%

Aerosol delivery of urocanic acid–modified chitosan/programmed cell death 4 complex regulated apoptosis, cell cycle, and angiogenesis in lungs of K-ras null mice

Jin

Kim

Hwang³

et al. 2006

Molecular Cancer Therapeutics

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The low efficiency of conventional therapies in achieving long-term survival of patients with lung cancer calls for development of novel treatment options. Although several genes have been investigated for their antitumor activities through gene delivery, problems surrounding the methods used, such as efficiency, specificity, and toxicity, hinder application of such therapies in clinical settings.

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“…26 To test whether CMCS-CLDPD could protect the loaded plasmid DNA from digestion by nucleases, they were exposed to DNase I at various time intervals. As illustrated in Figure 10 …”

Section: Stability Of Cmcs-cldpd Against Dnase Imentioning

confidence: 99%

A pH-sensitive multifunctional gene carrier assembled via layer-by-layer technique for efficient gene delivery

Liu²,

Miao³

et al. 2012

IJN

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Background:The success of gene therapy asks for the development of multifunctional vectors that could overcome various gene delivery barriers, such as the cell membrane, endosomal membrane, and nuclear membrane. Layer-by-layer technique is an efficient method with easy operation which can be used for the assembly of multifunctional gene carriers. This work describes a pH-sensitive multifunctional gene vector that offered long circulation property but avoided the inhibition of tumor cellular uptake of gene carriers associated with the use of polyethylene glycol. Methods: Deoxyribonucleic acid (DNA) was firstly condensed with protamine into a cationic core which was used as assembly template. Then, additional layers of anionic DNA, cationic liposomes, and o-carboxymethyl-chitosan (CMCS) were alternately adsorbed onto the template via layer-by-layer technique and finally the multifunctional vector called CMCS-cationic liposome-coated DNA/protamine/DNA complexes (CLDPD) was constructed. For in vitro test, the cytotoxicity and transfection investigation was carried out on HepG2 cell line. For in vivo evaluation, CMCS-CLDPD was intratumorally injected into tumor-bearing mice and the tumor cells were isolated for fluorescence determination of transfection efficiency. Results: CMCS-CLDPD had ellipsoidal shapes and showed "core-shell" structure which showed stabilization property in serum and effective protection of DNA from nuclease degradation. In vitro and in vivo transfection results demonstrated that CMCS-CLDPD had pH-sensitivity and the outermost layer of CMCS fell off in the tumor tissue, which could not only protect CMCS-CLDPD from serum interaction but also enhance gene transfection efficiency. Conclusion: These results demonstrated that multifunctional CMCS-CLDPD had pH-sensitivity, which may provide a new approach for the antitumor gene delivery.

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Degradable polyethylenimine-alt-poly(ethylene glycol) copolymers as novel gene carriers

Cited by 225 publications

References 44 publications

Urocanic acid-modified chitosan-mediated PTEN delivery via aerosol suppressed lung tumorigenesis in K-rasLA1 mice

Urocanic acid-modified chitosan-mediated PTEN delivery via aerosol suppressed lung tumorigenesis in K-rasLA1 mice

Aerosol delivery of urocanic acid–modified chitosan/programmed cell death 4 complex regulated apoptosis, cell cycle, and angiogenesis in lungs of K-ras null mice

A pH-sensitive multifunctional gene carrier assembled via layer-by-layer technique for efficient gene delivery

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