Degradable methacrylic acid-based synthetic hydrogel for subcutaneous islet transplantation

Kinney, Sean M.; Ortaleza, Krystal; Vlahos, Alexander E.; Sefton, Michael V.

doi:10.1016/j.biomaterials.2021.121342

Cited by 28 publications

(32 citation statements)

References 52 publications

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“…Our results highlight a new potential application of MAA-based biomaterials beyond exploitation of it’s vascular-regenerative properties. MAA-based biomaterials have been previously shown to promote angiogenesis[8,9] leading to its use in soft tissue repair[14,19] and islet transplantation[15,33]. Here we show that MAA-based hydrogels or a coating promote peripheral nerve growth.…”

Section: Discussionmentioning

confidence: 51%

Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of mice

Androschuk

Tam

Mahou

et al. 2022

Preprint

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Peripheral nerve innervation is essential for regulating tissue repair and regeneration. MAA-based biomaterials have been previously shown to promote angiogenesis. Here we show a new role for MAA-based biomaterials in promoting terminal axon nerve growth. Our results demonstrate that MAA-based biomaterials promote peripheral nerve growth in an Igf-1 and Shh dependent manner. The resulting nerves increased the sensitivity of treated mice paws to nociception. iDISCO clearing showed that MAA increased the presence of peripheral nerve structures in whole explants. MAA was also able to increase the expression of key neuronal markers and growth factors in a peripheral neuropathy model, the diabetic db/db mouse, suggesting that MAA-based biomaterials may be relevant to treatment of peripheral neuropathy. Moreover, in a peripheral neuropathy model, MAA was able to up-regulate the expression of growth factors for an extended duration suggesting MAA may prevent degeneration through an effect on factors that promote survival. As all tissues are innervated, MAA-based biomaterials could have broad applications in the promoting regeneration and preventing degeneration of peripheral nerves.

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Section: Discussionmentioning

confidence: 51%

Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of mice

Androschuk

Tam

Mahou

et al. 2022

Preprint

Self Cite

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“…Thus, denervation impaired the angiogenic effects of MAA as well as the nerve regeneration effects shown in Figure 5. angiogenesis [8,9] leading to its use in soft tissue repair [14,19] and islet transplantation [15,33]. Here we show that MAA-based hydrogels or a coating promote peripheral nerve growth.…”

Section: Maa-induced Blood Vessel Growth Is Nerve Dependentmentioning

confidence: 74%

Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of mice

et al. 2022

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“…Several additional models with various degrees of immune depletion have also been used for human islet transplant studies. This includes CB17.Cg- Prkdc scid Lyst bg-J /Crl (SCID beige) mice, a congenic line that results in defective NK cells, T cells, and B cells through mutations in Pkrdc and Lysbtg ( 79 , 83 , 84 ). Another immunocompromised mouse model occasionally used in human islet transplants is the CAnN.Cg- Foxn1 nu /Crl (BALB/c nu/nu) mouse model ( 85 , 86 ).…”

Section: Mouse Models Used For Transplantationmentioning

confidence: 99%

Mouse models and human islet transplantation sites for intravital imaging

et al. 2022

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Human islet transplantations into rodent models are an essential tool to aid in the development and testing of islet and cellular-based therapies for diabetes prevention and treatment. Through the ability to evaluate human islets in an in vivo setting, these studies allow for experimental approaches to answer questions surrounding normal and disease pathophysiology that cannot be answered using other in vitro and in vivo techniques alone. Intravital microscopy enables imaging of tissues in living organisms with dynamic temporal resolution and can be employed to measure biological processes in transplanted human islets revealing how experimental variables can influence engraftment, and transplant survival and function. A key consideration in experimental design for transplant imaging is the surgical placement site, which is guided by the presence of vasculature to aid in functional engraftment of the islets and promote their survival. Here, we review transplantation sites and mouse models used to study beta cell biology in vivo using intravital microscopy and we highlight fundamental observations made possible using this methodology.

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Degradable methacrylic acid-based synthetic hydrogel for subcutaneous islet transplantation

Cited by 28 publications

References 52 publications

Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of mice

Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of mice

Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of mice

Mouse models and human islet transplantation sites for intravital imaging

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