Degeneration of monoaminergic fibers in the aged micrencephalic rat

Watabe, Yoshiyuki; Yoshimoto, Kanji; Eguchi, Mitsuoki; Ueda, Seinosuke

doi:10.1016/j.neulet.2005.05.020

Cited by 10 publications

(15 citation statements)

References 26 publications

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“…1), a highly laminar pattern of distribution of serotonergic fibers was noted in the hippocampus and overlying cortex of MAM groups rats, which is consistent with our previous findings (Ueda et al 1999;Watabe et al 2005). The density of serotonergic fibers was highest in the stratum lacunosum-moleculare (SLM) of CA1, the stratum oriens and stratum radiatum (SR) of CA3 and the heterotopias, lower in the molecular layer (ML) of the dentate gyrus (DG) and lowest in the pyramidal cell layer (PCL) of CA3 and the granule cell layer (GCL) of the DG (Fig.…”

Section: -Ht Immunohistochemistrysupporting

confidence: 91%

“…Pregnant Sprague-Dawley (SD) rats (Japan Charles River Laboratories, Tsukuba, Japan) were intraperitoneally injected with either 25 mg/kg MAM (10 mg/ml physiological saline) or an equal volume of vehicle alone at gestational day 15 (Ueda et al 1999;Watabe et al 2005). At 3 days after birth, pups initially underwent intraperitoneal injection of desipramine (DMI; Sigma, St. Louis, MO; 20 mg/kg), a catecholamine uptake inhibitor.…”

Section: Animalsmentioning

confidence: 99%

“…Biochemical measurements using high-performance liquid chromatography (HPLC) were performed as described previously (Ueda et al 1999;Watabe et al 2005). Briefly, at age 14 weeks, rats (n = 5-6 in each group) were killed with a lethal overdose of sodium pentobarbital and decapitated.…”

Section: Biochemical Measurementsmentioning

confidence: 99%

“…Sections were collected in 0.1 M PBS containing 0.3% Triton-X 100, and incubated in 1% H 2 O 2 solution treated with 1.5% normal goat serum or normal horse serum at room temperature for 1 h to prevent non-specific staining. Sections were then incubated with primary antibodies, polyclonal anti-serotonin antiserum (1:10,000 dilution; Ueda et al 1999;Watabe et al 2005) for 4 days at 4°C. After rinsing several times in PBS, sections were processed for avidinbiotin peroxidase immunostaining and then reacted with 20 mg of 3,3 0 -diaminobenzidine (DAB) in 100 ml of 0.05 M Tris-HCl buffer (pH 7.4), containing 20 ll of 30% H 2 O 2 for 10 min.…”

Section: Tissue Preparationmentioning

confidence: 99%

“…Exposure of pregnant rats to MAM at gestational day 15 results in micrencephaly with cortical, periventricular and hippocampal heterotopias in offspring , as well as alteration of neurotransmitter systems in the brain (Johnston and Coyle 1982;Catabeni and Di Lica 1997). All of these heterotopias in the MAM-induced micrencephalic rat (MAM rat) receive excessive catecholaminergic, serotonergic and c-aminobutyric acid (GABA) innervations (Johnston and Coyle 1982;Catabeni and Di Lica 1997;Baraban et al 2000;Watabe et al 2005). Electrophysiological and neuroanatomical analyses have demonstrated that heterotopic neurons in the hippocampus display cellular properties of neocortical neurons with altered fiber connections Colacitti et al 1998) and atypical electrophysiological characteristics because of aberrant expression of ion channels and transmitter receptors Castro et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection

et al. 2009

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In order to elucidate the regeneration properties of serotonergic fibers in the hippocampus of methylazoxymethanol acetate (MAM)-induced micrencephalic rats (MAM rats), we examined serotonergic regeneration in the hippocampus following neonatal intracisternal 5,7-dihydroxytryptamine (5,7-DHT) injection. Prenatal exposure to MAM resulted in the formation of hippocampal heterotopia in the dorsal hippocampus. Immunohistochemical and neurochemical analyses revealed hyperinnervation of serotonergic fibers in the hippocampus of MAM rats. After neonatal 5,7-DHT injection, most serotonergic fibers in the hippocampus of 2-week-old MAM rats had degenerated, while a small number of serotonergic fibers in the stratum lacunosum-moleculare (SLM) of the hippocampus and in the hilus adjacent to the granular cell layer of the dentate gyrus (DG) had not. Regenerating serotonergic fibers from the SLM first extended terminals into the hippocampal heterotopia, then fibers from the hilus reinnervated the DG and some fibers extended to the heterotopia. These findings suggest that the hippocampal heterotopia exerts trophic target effects for regenerating serotonergic fibers in the developmental period in micrencephalic rats.

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Section: -Ht Immunohistochemistrysupporting

confidence: 91%

Section: Animalsmentioning

confidence: 99%

Section: Biochemical Measurementsmentioning

confidence: 99%

Section: Tissue Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection

et al. 2009

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Recovery and aging of serotonergic fibers after single and intermittent MDMA treatment in dark agouti rat

Ádori

Andó

Szekeres

et al. 2011

J of Comparative Neurology

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3,4-Methylenedioxymethamphetamine (MDMA; ecstasy) is a popular party drug known to cause selective serotonergic damage. Here we examined the long-term recovery and aging of serotonergic fibers and levels of brain-derived neurotrophic factor (BDNF) after intermittent MDMA administration (15 mg kg(-1) i.p. every 7th day for 4 weeks, MDMA ×4) and a single-dose treatment (15 mg kg(-1) i.p., MDMA ×1) in adolescent/young adult male Dark Agouti rats. After MDMA treatment, tryptophan hydroxylase-immunoreactive fiber density decreased and then recovered in all brain regions. Recovery was more pronounced in the MDMA ×4 group compared with the MDMA ×1 group, but similar long-term BDNF responses were found after both treatments. Twenty-two months after treatment, there were fewer clusters of aberrant serotonergic fibers in the parietal cortex in the MDMA ×4 group compared with the MDMA ×1 group. There was no difference in the density of microglial cells or astrocytes in treated groups versus the control 22 months after the treatments. These results indicate that recovery of serotonergic fibers is faster after intermittent MDMA treatment than after single-dose administration, and differences in BDNF levels per se are unlikely to account for this difference. Moreover, it seems that intermittent MDMA treatment attenuates the morphological signs of aging in serotonergic fibers. In addition, neither intermittent nor single-dose MDMA exposition of young animals induces accelerated aging processes or neurodegeneration in senescence, as indicated by the unaltered densities of microglial cells and astrocytes in the treated groups compared with the control.

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Acute urinary retention in two patients after subthalamic nucleus deep brain stimulation (STN‐DBS) for the treatment of advanced Parkinson's disease

et al. 2009

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3,4‐Methylenedioxymethamphetamine (MDMA; ecstasy) is a popular party drug known to cause selective serotonergic damage. Here we examined the long‐term recovery and aging of serotonergic fibers and levels of brain‐derived neurotrophic factor (BDNF) after intermittent MDMA administration (15 mg kg−1 i.p. every 7th day for 4 weeks, MDMA ×4) and a single‐dose treatment (15 mg kg−1 i.p., MDMA ×1) in adolescent/young adult male Dark Agouti rats. After MDMA treatment, tryptophan hydroxylase‐immunoreactive fiber density decreased and then recovered in all brain regions. Recovery was more pronounced in the MDMA ×4 group compared with the MDMA ×1 group, but similar long‐term BDNF responses were found after both treatments. Twenty‐two months after treatment, there were fewer clusters of aberrant serotonergic fibers in the parietal cortex in the MDMA ×4 group compared with the MDMA ×1 group. There was no difference in the density of microglial cells or astrocytes in treated groups versus the control 22 months after the treatments. These results indicate that recovery of serotonergic fibers is faster after intermittent MDMA treatment than after single‐dose administration, and differences in BDNF levels per se are unlikely to account for this difference. Moreover, it seems that intermittent MDMA treatment attenuates the morphological signs of aging in serotonergic fibers. In addition, neither intermittent nor single‐dose MDMA exposition of young animals induces accelerated aging processes or neurodegeneration in senescence, as indicated by the unaltered densities of microglial cells and astrocytes in the treated groups compared with the control. J. Comp. Neurol. 519:2353–2378, 2011. © 2011 Wiley‐Liss, Inc.

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Degeneration of monoaminergic fibers in the aged micrencephalic rat

Cited by 10 publications

References 26 publications

Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection

Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection

Recovery and aging of serotonergic fibers after single and intermittent MDMA treatment in dark agouti rat

Acute urinary retention in two patients after subthalamic nucleus deep brain stimulation (STN‐DBS) for the treatment of advanced Parkinson's disease

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