Deformed wing virus

Miranda, Joachim R. de; Genersch, Elke

doi:10.1016/j.jip.2009.06.012

Cited by 472 publications

(504 citation statements)

References 162 publications

Supporting

Mentioning

473

Contrasting

Unclassified

Order By: Relevance

“…Cuticular hydrocarbon profiles can be altered by activation of the immune system and their changes mediated through hygienic and undertaking behaviours in honeybees (Salvy et al, 2001;Richard et al, 2008;Evans and Spivak, 2010). No new compounds were found in the cuticular profiles of our infected bees and, according to Richard et al (2008), our results demonstrate that neither immune stimulation by Deformed Wing Viruses nor probably by the Varroa destructor (the main factor capable of triggering visible symptoms of DWV infected bees, (de Miranda and Genersch, 2010)) produces a specific cue for disease, but rather alters the overall cuticular hydrocarbon patterns in a non-specific way. On average, CHC profiles of healthy bees present a relatively greater abundance of low molecular weight compounds, while the DWV bees have more higher molecular weight compounds.…”

Section: Discussionmentioning

confidence: 61%

Evidence for antiseptic behaviour towards sick adult bees in honey bee colonies

Baracchi

Fadda

Turillazzi

2012

Journal of Insect Physiology

103

View full text Add to dashboard Cite

a b s t r a c tSocial life is generally associated with an increased risk of disease transmission, but at the same time it allows behavioural defence at both the individual and collective level. Bees infected with deformed-wing virus were introduced into observation hives; through behavioural observations and chemical analysis of cuticular hydrocarbons from healthy and infected bees, we offer the first evidence that honeybee colonies can detect and remove infected adult bees, probably by recognising the cuticular hydrocarbon profiles of sick individuals. We also found that health-compromised colonies were less efficient at defending themselves against infected bees, thus facing an ever increasing risk of epidemics. This work reveals a new antiseptic behaviour that can only be interpreted as an adaptation at colony level and one which should be considered an element of the social immunity system of the beehive, re-enforcing the view of a colony as an integrated organism.

show abstract

Section: Discussionmentioning

confidence: 61%

Evidence for antiseptic behaviour towards sick adult bees in honey bee colonies

Baracchi

Fadda

Turillazzi

2012

Journal of Insect Physiology

103

View full text Add to dashboard Cite

show abstract

“…DWV complex strains are closely related at proteolytic sites (de Miranda & Genersch 2010) and naturally recombine (Moore et al . 2011).…”

Section: Resultsmentioning

confidence: 99%

A sting in the spit: widespread cross‐infection of multipleRNAviruses across wild and managed bees

McMahon

Fürst

Caspar

et al. 2015

Journal of Animal Ecology

251

285

View full text Add to dashboard Cite

Summary Declining populations of bee pollinators are a cause of concern, with major repercussions for biodiversity loss and food security. RNA viruses associated with honeybees represent a potential threat to other insect pollinators, but the extent of this threat is poorly understood.This study aims to attain a detailed understanding of the current and ongoing risk of emerging infectious disease (EID) transmission between managed and wild pollinator species across a wide range of RNA viruses.Within a structured large‐scale national survey across 26 independent sites, we quantify the prevalence and pathogen loads of multiple RNA viruses in co‐occurring managed honeybee (Apis mellifera) and wild bumblebee (Bombus spp.) populations. We then construct models that compare virus prevalence between wild and managed pollinators.Multiple RNA viruses associated with honeybees are widespread in sympatric wild bumblebee populations. Virus prevalence in honeybees is a significant predictor of virus prevalence in bumblebees, but we remain cautious in speculating over the principle direction of pathogen transmission. We demonstrate species‐specific differences in prevalence, indicating significant variation in disease susceptibility or tolerance. Pathogen loads within individual bumblebees may be high and in the case of at least one RNA virus, prevalence is higher in wild bumblebees than in managed honeybee populations.Our findings indicate widespread transmission of RNA viruses between managed and wild bee pollinators, pointing to an interconnected network of potential disease pressures within and among pollinator species. In the context of the biodiversity crisis, our study emphasizes the importance of targeting a wide range of pathogens and defining host associations when considering potential drivers of population decline.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Recently, it has been shown that DWV infections can be vertically transmitted through drones and queens and that this transmission route results in covert infections (presence of the virus in the absence of visible symptoms) (de Miranda & Genersch, 2010;de Miranda & Fries, 2008;Yue et al, 2007), which follows the general assumption that vertical transmission selects for less virulent forms of a pathogen (Ewald, 1983;Ewald & De Leo, 2002;Fries & Camazine, 2001). These vertically infected bees also lacked another hallmark of overt DWV infection, the detection of DWV RNA in bees' heads (Yue & Genersch, 2005;Yue et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Overt DWV infections characterized by the occurrence of visible disease symptoms (deformed wings, bloated and shortened abdomen, and miscolouring) are associated with V. destructor infestation (Ball & Allen, 1988;Bowen-Walker et al, 1999;Martin, 2001;Martin et al, 1998; Santillán-Galicia et al, 2010;Tentcheva et al, 2006;Yue & Genersch, 2005). However, the exact role of the mite in the pathogenesis of DWV is still not fully understood and a direct causal link between the virus and the symptom 'wing deformity' has not been established yet (de Miranda & Genersch, 2010).Recently, it has been shown that DWV infections can be vertically transmitted through drones and queens and that this transmission route results in covert infections (presence of the virus in the absence of visible symptoms) (de Miranda & Genersch, 2010;de Miranda & Fries, 2008;Yue et al, 2007), which follows the general assumption that vertical transmission selects for less virulent forms of a pathogen (Ewald, 1983;Ewald & De Leo, 2002;Fries & Camazine, 2001). These vertically infected bees also lacked another hallmark of overt DWV infection, the detection of DWV RNA in bees' heads (Yue & Genersch, 2005;Yue et al, 2007).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Horizontal transmission of deformed wing virus: pathological consequences in adult bees (Apis mellifera) depend on the transmission route

Möckel

Gisder

Genersch

2010

Journal of General Virology

159

191

View full text Add to dashboard Cite

Recent reports on a steady decline of honeybee colonies in several parts of the world caused great concern. There is a consensus that pathogens are among the key players in this alarming demise of the most important commercial pollinator. One of the pathogens heavily implicated in colony losses is deformed wing virus (DWV). Overt DWV infections manifested as deformed-wing syndrome started to become a threat to honeybees only in the wake of the ectoparasitic mite Varroa destructor, which horizontally transmits DWV. However, a direct causal link between the virus and the symptom 'wing deformity' has not been established yet. To evaluate the impact of different horizontal transmission routes, and especially the role of the mite in the development of overt DWV infections, we performed laboratory infection assays with pupae and adult bees. We could demonstrate that pupae injected with DWV dose-dependently developed overt infections characterized by deformed wings in adult bees, suggesting that DWV, if transmitted to pupae by the mite, is the causative agent of the deformed-wing syndrome. The OID 50 (overt infection dosage) was approximately 2500 genome equivalents. Injecting more than 1¾10 7 DWV genome equivalents into adult bees also resulted in overt infections while the same viral dosage fed to adult bees only resulted in covert infections. Therefore, both infection of adult bees through DWVtransmitting phoretic mites and infection of nurse bees through their cannibalizing DWV-infected pupae might represent possible horizontal transmission routes of DWV. INTRODUCTIONManaged honeybees are indispensable for profitable and sustainable agriculture and for many non-agricultural ecosystems because of the extraordinary pollination service they provide. Hence, reports on a steady decline in honeybee populations in Europe, USA and Japan in the recent past (for a review see vanEngelsdorp & Meixner, 2010) caused great concern and several studies were initiated to identify the underlying cause. From these studies it became more and more evident that the key players in most of the 'inexplicable' colony losses are pathogens (Ratnieks & Carreck, 2010). Especially, the ectoparasitic mite Varroa destructor in concert with certain pathogenic bee viruses played a major role in the observed colony collapses (Cox-Foster et al., 2007;Genersch et al., 2010; Guzmán-Novoa et al., 2010;Highfield et al., 2009;Siede et al., 2008;vanEngelsdorp, 2008;vanEngelsdorp et al., 2009).One of the viruses heavily implicated in colony losses is Deformed wing virus (DWV), a plus-stranded RNA virus belonging to the genus Iflaviridae (Lanzi et al., 2006). DWV normally causes covert infections in honeybees (de Miranda & Fries, 2008;Hails et al., 2008;Yue et al., 2007). Overt DWV infections characterized by the occurrence of visible disease symptoms (deformed wings, bloated and shortened abdomen, and miscolouring) are associated with V. destructor infestation (Ball & Allen, 1988;Bowen-Walker et al., 1999;Martin, 2001;Martin et al., 1998; Santillán-Galicia et...

show abstract

Deformed wing virus

Cited by 472 publications

References 162 publications

Evidence for antiseptic behaviour towards sick adult bees in honey bee colonies

Evidence for antiseptic behaviour towards sick adult bees in honey bee colonies

A sting in the spit: widespread cross‐infection of multipleRNAviruses across wild and managed bees

Horizontal transmission of deformed wing virus: pathological consequences in adult bees (Apis mellifera) depend on the transmission route

Contact Info

Product

Resources

About