Deformed wing virus (DWV) is a virulent and ubiquitous disease that affects honey bee colonies. DWV infects bees at all life stages, but is most noticeable in adult bees, where clinical symptoms include shriveled, non-functional wings, and a drastically shortened lifespan. DWV is recorded in upwards of 90% of honey bee colonies worldwide, and has been linked to colony loss in symptomatic hives. DWV is primarily spread byVarroa destructormites, who feed on the fat bodies of honey bee adults and pupae. There is currently no direct treatment or preventative for DWV, with the primary method of reduction being vector control using acaracides. In this study, we tested the effect that vaccinating honey bee queens using killedPaenibacillus larvaebacterin, the causative agent of the honey bee disease American Foulbrood, had on deformed wing virus load in honey bee colonies. We placed vaccinated queens in 200 honey bee colonies, and unvaccinated queens in 200 colonies, and measured quantities of DWV-B in both groups immediately before, and 4 months after vaccination. We found that levels of DWV-B were identical before vaccination, but were significantly reduced in colonies 4 months post vaccination. This change was found despite no difference in mite quantities between groups. Overall, these data provide evidence that vaccination of queens withP. larvaebacterin is an effective method for reduction of DWV-B quantities in honey bee colonies in a commercially relevant field setting.