Deformation of human erythrocytes in a centrifugal field

Corry, W. D.; Meiselman, H.J.

doi:10.1016/s0006-3495(78)85506-4

Cited by 18 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Red cell deformability has been measured by the micropipette technique (EVANS and LACELLE, 1975), filtration (CHIEN, 1977), resistive pulse spectroscopy (MEL andYEE, 1975), automated ektacytometer (BEssis et al, 1980), centrifugal elongation (CORRY and MEISELMAN, 1978 ;NAGASAWA et al, 1980), and so forth . According to the ektacytometric study (ALLARD et al, 1978), red cell deformability was regulated by three factors: internal viscosity, surface area to volume ratio, and viscoelastic modules of the membrane.…”

Section: Discussionmentioning

confidence: 99%

Coil planet centrifugal and capillary tube centrifugal analysis of factors regulating erythrocyte osmotic fragility and deformability.

1982

View full text Add to dashboard Cite

Hydrated and dehydrated red cell samples were prepared from normal human red cells using the antibiotic nystatin. Furthermore, a series of red cell samples exposed to elevated temperature (20-50°C, 10 min) were prepared. The osmotic fragility and deformability of these red cells were then measured, using the coil planet centrifuge system and the capillary tube centrifugal technique, respectively. The osmotic fragility of nystatin-treated red cells decreased and the deformability increased as dehydration of red cells progressed and alternatively, hydrated cells showed increased osmotic fragility and reduced deformability. Red cells exposed to elevated temperatures up to 49°C for 10 min had no changes in mean corpuscular volume or in red cell shape. Above 47°C, however, spectrin extractability progressively decreased and osmotic fragility and deformability decreased. Results suggest that the osmotic fragility and deformability of red cells are interrelated, and are controlled by the geometry of the cell, including the ratio of cell surface area to cell volume and the viscoelastic properties of the membrane.

show abstract

Section: Discussionmentioning

confidence: 99%

Coil planet centrifugal and capillary tube centrifugal analysis of factors regulating erythrocyte osmotic fragility and deformability.

1982

View full text Add to dashboard Cite

show abstract

“…Centrifugation deforms cell shape and vast majority of cells resemble spheres with a flat, triangular tail (Corry & Meiselman, 1978). Centrifugation deforms cell shape and vast majority of cells resemble spheres with a flat, triangular tail (Corry & Meiselman, 1978).…”

Section: Mr Studiesmentioning

confidence: 99%

“…Diffusion anisotropy in red blood cells might be a concern measuring in one direction only, but diffusion anisotropy was proven only in cell suspensions and we and others (P. W. Kuchel, personal communication) could not confirm the existance of diffusion anisotropy in red blood cells after centrifugation. Centrifugation deforms cell shape and vast majority of cells resemble spheres with a flat, triangular tail (Corry & Meiselman, 1978).…”

Section: Mr Studiesmentioning

confidence: 99%

Osmotic and diffusive properties of intracellular water in camel erythrocytes: Effect of hemoglobin crowdedness

Bogner

Miseta

Berente

et al. 2005

Cell Biology International

View full text Add to dashboard Cite

Camel erythrocytes have exceptional osmotic resistance and is believed to be due to augmented water-binding associated with the high hydrophilicity of camel hemoglobin. In practical terms this means that the proportion of osmotically non-removable water in camel erythrocytes is nearly 3-fold greater than that in human erythrocytes (approximately 65 vs approximately 20%). The relationship between water diffusion and the osmotic characteristics of intracellular water is the subject of this report. The amount of osmotically inactive water is 2-fold greater in camel hemoglobin solution in vitro compared to that of human, but water diffusion does not differ in camel and human hemoglobin solutions. However, the evaluation of water diffusion by magnetic resonance measurements in camel erythrocytes revealed approximately 15% lower apparent diffusion coefficient (ADC) compared with human erythrocytes. When human erythrocytes were dehydrated to the level of camel erythrocytes, their osmotic and water diffusion properties were similar. These results show that a lower ADC is associated with a more pronounced increase in osmotically inactive water fraction. It is proposed that increased hemoglobin hydrophilicity allows not only augmented water-binding, but also a closer hemoglobin packaging in vivo, which in turn is associated with slower ADC and increased osmotic resistance.

show abstract

“…This finding was interpreted to indicate that normal cells, being more deformable, packed to a greater degree. A novel variation of this scheme involves centrifuging, horizontally, a dilute suspension of biconcave human red blood cells for 5 sec (2-15,000 ϫ g) through a Teflon capillary tube into a 2% acetaldehyde fixation solution (10,11). The idea was that any deformation of the cells that took place while in the capillary would be preserved on contact with the fixation solution.…”

mentioning

confidence: 99%

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human andAmphiumared blood cells

Hoffman¹,

Inoué²

2008

Collected Works of Shinya Inoué

View full text Add to dashboard Cite

This paper describes changes that occur in human and Amphiuma red blood cells observed during centrifugation with a special microscope. Dilute suspensions of cells were layered, in a centrifuge chamber, above an osmotically matched dense solution, containing Nycodenz, Ficoll, or Percoll (Pharmacia) that formed a density gradient that allowed the cells to slowly settle to an equilibrium position. Biconcave human red blood cells moved downward at low forces with minimum wobble. The cells oriented vertically when the force field was increased and Hb sedimented as the lower part of each cell became bulged and assumed a ''baglike'' shape. The upper centripetal portion of the cell became thinner and remained biconcave. These changes occurred rapidly and were completely reversible upon lowering the centrifugal force. Bag-shaped cells, upon touching red cells in rouleau, immediately reverted to biconcave disks as they flipped onto a stack. Amphiuma red cells displayed a different type of reversible stratification and deformation at high force fields. Here the cells became stretched, with the nucleus now moving centrifugally, the Hb moving centripetally, and the bottom of the cells becoming thinner and clear. Nevertheless, the distribution of the marginal bands at the cells' rim was unchanged. We conclude that centrifugation, per se, while changing a red cell's shape and the distribution of its intracellular constituents, does so in a completely reversible manner. Centrifugation of red cells harboring altered or missing structural elements could provide information on shape determinants that are still unexplained. centrifuge polarization microscope ͉ Hb sedimentation ͉ shape deformations Conflict of interest statement: No conflicts declared.

show abstract

Deformation of human erythrocytes in a centrifugal field

Cited by 18 publications

References 13 publications

Coil planet centrifugal and capillary tube centrifugal analysis of factors regulating erythrocyte osmotic fragility and deformability.

Coil planet centrifugal and capillary tube centrifugal analysis of factors regulating erythrocyte osmotic fragility and deformability.

Osmotic and diffusive properties of intracellular water in camel erythrocytes: Effect of hemoglobin crowdedness

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human andAmphiumared blood cells

Contact Info

Product

Resources

About