Deformation of erythrocytes under shear: A small-angle light scattering study

Mazeron, Paul; Müller, Stéphane; Azouzi, H. El

doi:10.1016/s0006-355x(97)00007-3

Cited by 20 publications

(10 citation statements)

References 24 publications

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“…Keller and Skalak have modeled the behavior of nondeforming ellipsoidal particles in shear flow and predicted the shear rate at which the transition to tank treading occurs. 8 The average deformation of a suspension of red blood cells was most recently measured by Mazeron et al 9 Chang and Olbrecht have measured the deformation of a single synthetic cell whose membrane is viscoelastic in extensional and Couette flows. 10,11 The flow of deformable RBCs through capillaries can be considered axisymmetric under appropriate conditions and has been studied theoretically.…”

Section: Introductionmentioning

confidence: 99%

Large deformation of red blood cell ghosts in a simple shear flow

1998

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Red blood cells are known to change shape in response to local flow conditions. Deformability affects red blood cell physiological function and the hydrodynamic properties of blood. The immersed boundary method is used to simulate three-dimensional membrane-fluid flow interactions for cells with the same internal and external fluid viscosities. The method has been validated for small deformations of an initially spherical capsule in simple shear flow for both neoHookean and the Evans-Skalak membrane models. Initially oblate spheroidal capsules are simulated and it is shown that the red blood cell membrane exhibits asymptotic behavior as the ratio of the dilation modulus to the extensional modulus is increased and a good approximation of local area conservation is obtained. Tank treading behavior is observed and its period calculated.

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Section: Introductionmentioning

confidence: 99%

Large deformation of red blood cell ghosts in a simple shear flow

1998

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“…Therefore, the shear stress was calculated to be 133 dyn/cm 2 by the formula: shear stress ¼ (pressure difference 3 radius of tube)/ (2 3 length of tube). The similar rate of share stress has been well used for the erythrocyte-elongation by the ektacytometer, to simulate the physiological condition (Mazeron et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

Dynamic study of intramembranous particles in human fresh erythrocytes using an “in vitro cryotechnique”

Terada

Ohno

Fujii

et al. 2006

Microsc. Res. Tech.

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For analyses of dynamic ultrastructures of erythrocyte intramembranous particles (IMPs) in situ, a quick-freezing method was used to stabilize the flow behavior of erythrocytes embedded in vitreous ice. Fresh human blood was jetted at various pressures through artificial tubes, in which the flowing erythrocytes were elongated from biconcave discoid shapes to elliptical ones, and quickly frozen in liquid isopentane-propane cryogen (-193 degrees C). They were freeze-fractured using a scalpel in liquid nitrogen, and routinely prepared for replica membranes. Many IMPs were observed on the protoplasmic freeze-fracture face (P-face) of the erythrocyte membranes. Some control erythrocytes under nonflowing or stationary conditions showed IMPs with their random distribution. However, other jetted erythrocytes under flowing conditions showed variously sized IMPs with much closer distribution. They were also arranged into parallel rows in some parts, and aggregated together. This quick-freezing method enabled for the first time the visualization of time-dependent topology and the molecular alteration of IMPs in dynamically flowing erythrocytes.

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“…Numerical estimations show that when n s /n 0 is equal to 1.05 (difference of ~ 5%), trapping efficiency reaches about 60% of maximum possible efficacy. In practice, this corresponds to a RBC in a phosphate buffer solution (PBS) 37 , which also shows a relatively stable trap and allows carrying out measurements 9,38 .…”

Section: Discussionmentioning

confidence: 99%

Optical tweezers-assisted measurements of elastic light scattering

et al. 2014

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Optical tweezers have been used in biophysical studies for over twenty years. Typical application areas are force measurements of subcellular structures and cell biomechanics. Optical tweezers can also be used to manipulate the orientation of objects. Moreover, using various beam shapes, optical tweezers allow measuring light scattering from single and multiple objects by keeping particles and cells in place during the measurement. At single cell level, light scattering yields important information about the object being studied, including its size, shape and refractive index. Also dependent scattering can be studied. In this paper, we review experimental work conducted in this area by our group and show new results relating to optical clearing phenomena at single microparticle level.

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Deformation of erythrocytes under shear: A small-angle light scattering study

Cited by 20 publications

References 24 publications

Large deformation of red blood cell ghosts in a simple shear flow

Large deformation of red blood cell ghosts in a simple shear flow

Dynamic study of intramembranous particles in human fresh erythrocytes using an “in vitro cryotechnique”

Optical tweezers-assisted measurements of elastic light scattering

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