Deformation of caveolae impacts global transcription and translation processes through relocalization of cavin-1

Qifti, Androniqi; Balaji, Shravani; Scarlata, Suzanne

doi:10.1016/j.jbc.2022.102005

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…For example, cavin proteins are released from the PM when caveolae are disassembled. Cavin1 has been localized in the cell nucleus, where it regulates rRNA transcription in response to insulin stimulation and osmotic stress [ 99 , 100 ]. Cavin3 could also enter the nucleus when caveolae are disassembled by ultraviolet light exposure, and nuclear Cavin3 regulates DNA damage and apoptosis through the interaction with the phosphatase PP1α [ 101 ] and BRCA1 [ 102 ].…”

Section: Caveolae: Composition Organization and Functionmentioning

confidence: 99%

Caveolae Mechanotransduction at the Interface between Cytoskeleton and Extracellular Matrix

Sotodosos-Alonso

Pulgarín-Alfaro

Pozo

2023

Cells

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The plasma membrane (PM) is subjected to multiple mechanical forces, and it must adapt and respond to them. PM invaginations named caveolae, with a specific protein and lipid composition, play a crucial role in this mechanosensing and mechanotransduction process. They respond to PM tension changes by flattening, contributing to the buffering of high-range increases in mechanical tension, while novel structures termed dolines, sharing Caveolin1 as the main component, gradually respond to low and medium forces. Caveolae are associated with different types of cytoskeletal filaments, which regulate membrane tension and also initiate multiple mechanotransduction pathways. Caveolar components sense the mechanical properties of the substrate and orchestrate responses that modify the extracellular matrix (ECM) according to these stimuli. They perform this function through both physical remodeling of ECM, where the actin cytoskeleton is a central player, and via the chemical alteration of the ECM composition by exosome deposition. Here, we review mechanotransduction regulation mediated by caveolae and caveolar components, focusing on how mechanical cues are transmitted through the cellular cytoskeleton and how caveolae respond and remodel the ECM.

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Section: Caveolae: Composition Organization and Functionmentioning

confidence: 99%

Caveolae Mechanotransduction at the Interface between Cytoskeleton and Extracellular Matrix

Sotodosos-Alonso

Pulgarín-Alfaro

Pozo

2023

Cells

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“…Notably, our STED-CLEM approach cannot evaluate quantitative changes in protein levels at caveolae membrane sites. Consequently, different results will be observed compared to other analysis of cavin plasma membrane localization and cavin-caveolin1 40 , 49 , 61 or caveolin1-caveolin1 62 , 63 association after osmotic shock. Future experiments are needed to dismantle these discrepancies.…”

Section: Discussionmentioning

confidence: 94%

The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane

et al. 2022

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Caveolae are small coated plasma membrane invaginations with diverse functions. Caveolae undergo curvature changes. Yet, it is unclear which proteins regulate this process. To address this gap, we develop a correlative stimulated emission depletion (STED) fluorescence and platinum replica electron microscopy imaging (CLEM) method to image proteins at single caveolae. Caveolins and cavins are found at all caveolae, independent of curvature. EHD2 is detected at both low and highly curved caveolae. Pacsin2 associates with low curved caveolae and EHBP1 with mostly highly curved caveolae. Dynamin is absent from caveolae. Cells lacking dynamin show no substantial changes to caveolae, suggesting that dynamin is not directly involved in caveolae curvature. We propose a model where caveolins, cavins, and EHD2 assemble as a cohesive structural unit regulated by intermittent associations with pacsin2 and EHBP1. These coats can flatten and curve to enable lipid traffic, signaling, and changes to the surface area of the cell.

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Emerging Insights into the Molecular Architecture of Caveolin-1

Ohi

Kenworthy

2022

J Membrane Biol

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Deformation of caveolae impacts global transcription and translation processes through relocalization of cavin-1

Cited by 4 publications

References 45 publications

Caveolae Mechanotransduction at the Interface between Cytoskeleton and Extracellular Matrix

Caveolae Mechanotransduction at the Interface between Cytoskeleton and Extracellular Matrix

The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane

Emerging Insights into the Molecular Architecture of Caveolin-1

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