Deformation-based brain morphometry in rats

Gaser, Christian; Schmidt, S.; Metzler, Martin; Herrmann, Karl‐Heinz; Krumbein, Ines; Reichenbach, Jürgen R.; Witte, Otto W.

doi:10.1016/j.neuroimage.2012.06.066

Cited by 33 publications

(30 citation statements)

References 61 publications

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“…Accordingly, all rat brains were parcellated into 150 regions of interest (ROIs) covering almost the entire brain (75 ROIs for each hemisphere, Fig 1) using a pre-existing template [39], which are defined in Paxinos space [22]. These were then nonlinearly normalized to the rat brain template used for this study [34].…”

Section: Methodsmentioning

confidence: 99%

Characterization of the resting-state brain network topology in the 6-hydroxydopamine rat model of Parkinson’s disease

et al. 2017

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Resting-state functional MRI (rsfMRI) is an imaging technology that has recently gained attention for its ability to detect disruptions in functional brain networks in humans, including in patients with Parkinson’s disease (PD), revealing early and widespread brain network abnormalities. This methodology is now readily applicable to experimental animals offering new possibilities for cross-species translational imaging. In this context, we herein describe the application of rsfMRI to the unilaterally-lesioned 6-hydroxydopamine (6-OHDA) rat, a robust experimental model of the dopamine depletion implicated in PD. Using graph theory to analyse the rsfMRI data, we were able to provide meaningful and translatable measures of integrity, influence and segregation of the underlying functional brain architecture. Specifically, we confirm that rats share a similar functional brain network topology as observed in humans, characterised by small-worldness and modularity. Interestingly, we observed significantly reduced functional connectivity in the 6-OHDA rats, primarily in the ipsilateral (lesioned) hemisphere as evidenced by significantly lower node degree, local efficiency and clustering coefficient in the motor, orbital and sensorimotor cortices. In contrast, we found significantly, and bilaterally, increased thalamic functional connectivity in the lesioned rats. The unilateral deficits in the cortex are consistent with the unilateral nature of this model and further support the validity of the rsfMRI technique in rodents. We thereby provide a methodological framework for the investigation of brain networks in other rodent experimental models of PD, as well as of animal models in general, for cross-comparison with human data.

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Section: Methodsmentioning

confidence: 99%

Characterization of the resting-state brain network topology in the 6-hydroxydopamine rat model of Parkinson’s disease

et al. 2017

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“…Therefore, we used T2‐weighted images in this study. T2‐weighted images have been used in previous VBM (Sawiak et al ., ; Biedermann et al ., ) and deformation‐based morphometry (Lerch et al ., ; Gaser et al ., ) studies in rodents. The T2‐weighted images were obtained using the respiration‐gated 2D‐rapid acquisition with refocused echoes (RARE) sequence with the following parameters: repetition time = 4628 ms, effective echo time = 30 ms, RARE factor = 4, flip angle = 90 degrees, field of view = 18 × 18 mm 2 , matrix size = 144 × 144, voxel size = 125 × 125 m 2 , number of slices = 54, slice thickness = 300 μm and number of averages = 20.…”

Section: Methodsmentioning

confidence: 99%

Specific regions display altered grey matter volume in μ‐opioid receptor knockout mice: MRI voxel‐based morphometry

Sasaki

Sumiyoshi

Nonaka

et al. 2014

British J Pharmacology

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BACKGROUND AND PURPOSEμ Opioid receptor knockout (MOP-KO) mice display several behavioural differences from wild-type (WT) littermates including differential responses to nociceptive stimuli. Brain structural changes have been tied to behavioural alterations noted in transgenic mice with targeting of different genes. Hence, we assess the brain structure of MOP-KO mice. EXPERIMENTAL APPROACHMagnetic resonance imaging (MRI) voxel-based morphometry (VBM) and histological methods were used to identify structural differences between extensively backcrossed MOP-KO mice and WT mice. KEY RESULTSMOP-KO mice displayed robust increases in regional grey matter volume in olfactory bulb, several hypothalamic nuclei, periaqueductal grey (PAG) and several cerebellar areas, most confirmed by VBM analysis. The largest increases in grey matter volume were detected in the glomerular layer of the olfactory bulb, arcuate nucleus of hypothalamus, ventrolateral PAG (VLPAG) and cerebellar regions including paramedian and cerebellar lobules. Histological analyses confirm several of these results, with increased VLPAG cell numbers and increased thickness of the olfactory bulb granule cell layer and cerebellar molecular and granular cell layers. CONCLUSIONS AND IMPLICATIONSMOP deletion causes previously undescribed structural changes in specific brain regions, but not in all regions with high MOP receptor densities (e.g. thalamus, nucleus accumbens) or that exhibit adult neurogenesis (e.g. hippocampus). Volume differences in hypothalamus and PAG may reflect behavioural changes including hyperalgesia. Although the precise relationship between volume change and MOP receptor deletion was not determined from this study alone, these findings suggest that levels of MOP receptor expression may influence a broader range of neural structure and function in humans than previously supposed.

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“…Several studies have looked at cerebral asymmetry in both rodents and humans. In rodents, significant cerebral asymmetry has been found mainly in the hippocampus, but the asymmetry seems to vary by strain, gender, and age (24)(25)(26). In human studies, hemispheric asymmetry of the whole hippocampus in healthy adults was also observed, with larger volume in the right hemisphere (27,28).…”

Section: Discussionmentioning

confidence: 99%

Atrophy associated with tau pathology precedes overt cell death in a mouse model of progressive tauopathy

Fung

Guo

Figueroa

et al. 2020

Preprint

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In the early stages of Alzheimer's disease (AD), tau pathology first develops in the entorhinal cortex (EC), then spreads to the hippocampus and at later stages, to the neocortex.Pathology in the neocortex correlates with impaired cognitive performance. Overall, tau pathology correlates well with neurodegeneration but the spatial and temporal association between tau pathology and overt volume loss is unclear. Using in vivo magnetic resonance imaging (MRI) with tensor-based morphometry (TBM) we mapped the spatio-temporal pattern of structural changes in a mouse model of AD-like progressive tauopathy. A novel, co-registered in vivo MRI atlas identified particular regions in the medial temporal lobe (MTL) that had significant volume reduction. The medial entorhinal cortex (MEC) and pre-/para-subiculum (PPS) had the most significant atrophy at the early stage, but atrophy then spread into the hippocampus, most notably, the CA1, dentate gyrus (DG) and subiculum (Sub). TBM-related atrophy in the DG and Sub preceded overt cell loss that has been reported in ex vivo studies in the same mouse model. By unifying an ex vivo 3D reconstruction of tau pathology with the TBM-MRI results we mapped the progression of atrophy in the MTL with the corresponding spread of tau pathology. Our study shows that there is an association between the spread of tau pathology and TBM-related atrophy from the EC to the hippocampus, but atrophy in the DG and Sub preceded overt cell loss.

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Deformation-based brain morphometry in rats

Cited by 33 publications

References 61 publications

Characterization of the resting-state brain network topology in the 6-hydroxydopamine rat model of Parkinson’s disease

Characterization of the resting-state brain network topology in the 6-hydroxydopamine rat model of Parkinson’s disease

Specific regions display altered grey matter volume in μ‐opioid receptor knockout mice: MRI voxel‐based morphometry

Atrophy associated with tau pathology precedes overt cell death in a mouse model of progressive tauopathy

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