Deformation analysis of gellan-gum based bone scaffold using on-the-fly tomography

Kytýř, Daniel; Zlámal, Petr; Koudelka, Petr; Fíla, Tomáš; Krčmářová, Nela; Kumpová, Ivana; Vavřı́k, Daniel; Gantar, Ana; Novak, Saša

doi:10.1016/j.matdes.2017.08.036

Cited by 28 publications

(26 citation statements)

References 47 publications

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Section: Biomechanical Xct Analysis Of Bone Regenerationmentioning

confidence: 97%

“…Kytýř et al . () studied the real‐time deformation response of a hydrogel‐bioglass composite, overcoming the limitations of time‐lapsed XCT mechanical testing for imaging compliant materials. On the other hand, Bormann et al .…”

Section: Biomechanical Xct Analysis Of Bone Regenerationmentioning

confidence: 99%

Section: Digital Volume Correlationmentioning

confidence: 99%

“…In the field of biomaterials for bone regeneration, XCT biomechanical imaging and DVC has shown great potential to evaluate strain distribution in polymeric and metallic scaffolds (Bormann et al, 2014;Kytýř et al, 2017). Kytýř et al (2017) studied the real-time deformation response of a hydrogel-bioglass composite, overcoming the limitations of time-lapsed XCT mechanical testing for imaging compliant materials. On the other hand, Bormann et al (2014) investigated local strains in a porous 3D printed metallic scaffold, demonstrating that DVC-computed strains may allow not only for an optimisation of the scaffold design, but also to better understand how mechanical stimuli may affect cell proliferation within the scaffolds.…”

Section: Digital Volume Correlationmentioning

confidence: 99%

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Applications of X‐ray computed tomography for the evaluation of biomaterial‐mediated bone regeneration in critical‐sized defects

Fernández

Witte²,

Tozzi

2019

Journal of Microscopy

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Bone as such displays an intrinsic regenerative potential following fracture; however, this capacity is limited with large bone defects that cannot heal spontaneously. The management of critical‐sized bone defects remains a major clinical and socioeconomic need with osteoregenerative biomaterials constantly under development aiming at promoting and enhancing bone healing. X‐ray computed tomography (XCT) has become a standard and essential tool for quantifying structure–function relationships in bone and biomaterials, facilitating the development of novel bone tissue engineering strategies. This paper presents recent advancements in XCT analysis of biomaterial‐mediated bone regeneration. As a noninvasive and nondestructive technique, XCT allows for qualitative and quantitative evaluation of three‐dimensional (3D) scaffolds and biomaterial microarchitecture, bone growth into the scaffold as well as the 3D characterisation of biomaterial degradation and bone regeneration in vitro and in vivo. Furthermore, in combination with in situ mechanical testing and digital volume correlation (DVC), XCT demonstrated its potential to better understand the bone–biomaterial interactions and local mechanics of bone regeneration during the healing process in relation to the regeneration achieved in vivo, which will likely provide valuable knowledge for the development and optimisation of novel osteoregenerative biomaterials. Lay Description Bone, being a dynamically adaptable material, displays excellent regenerative properties following fracture. However, the self‐healing capacity of bone becomes more difficult with large bone defects. Those defects are common and occur in many clinical situations; hence, biomaterials are mostly used to restore both bone structure and function in the defect site. X‐ray computed tomography (XCT) is a powerful tool to evaluate bone regeneration in critical‐sized defects after the implantation of biomaterials, allowing to an improved understanding of the regeneration process following different bone tissue engineering approaches. This paper focuses on recent advancements in XCT analysis to characterise biomaterial‐mediated bone regeneration in critical‐sized defects. XCT supports three‐dimensional (3D) analysis of biomaterials, scaffolds and regenerated bone microarchitecture, as well as bone ingrowth into the scaffold. As a nondestructive technique, XCT allows for a 3D characterisation of biomaterial degradation and bone regeneration over time. In addition, XCT combined with in situ mechanical experiments and digital volume correlation (DVC) provides a 3D evaluation and quantification of bone–biomaterial interactions and deformation mechanisms during the regeneration process. This remains essential for the development and enhancement of novel biomaterials able to produce bone that is comparable with the native tissue they aim to replace.

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Section: Biomechanical Xct Analysis Of Bone Regenerationmentioning

confidence: 97%

Section: Biomechanical Xct Analysis Of Bone Regenerationmentioning

confidence: 99%

Section: Digital Volume Correlationmentioning

confidence: 99%

Section: Digital Volume Correlationmentioning

confidence: 99%

See 2 more Smart Citations

Applications of X‐ray computed tomography for the evaluation of biomaterial‐mediated bone regeneration in critical‐sized defects

Fernández

Witte²,

Tozzi

2019

Journal of Microscopy

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“…The second high power XWT 240 SE (X-RAY WorX, Germany) 280 W reflection tube allows to analyse physically large samples or samples of materials with high attenuation of X-rays. Furthermore, it is possible to use different types of radiation imaging detectors -scintillating large-area flat panel detector, or various semiconductor pixelated detectors of the Medipix family [6,7] with theoretically unlimited dynamic range, including the large area detector composed of 10 × 10 Timepix 2 assembly [8], fast devices with maximum possible frame rate of 200 fps without compression in two-row/twocolumn configuration of Timepix chips [9], and also spectroscopic detectors. For the analysis presented in this paper, a combination of the XWT 160 source and XRD1622 (PerkinElmer, USA) scintillating large-area flat panel detector was used.…”

Section: X-ray Imaging Devicementioning

confidence: 99%

Instrumentation of Four-Point Bending Test During 4d Computed Tomography

Kytýř

Fíla

Koudelka

et al. 2018

APP

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High-resolution time-lapse micro-focus X-ray computed tomography is an effective method for investigation of deformation processes on volumetric basis including fracture propagation characteristics of non-homogeneous materials subjected to mechanical loading. This experimental method requires implementation of specifically designed loading devices to X-ray imaging setups. In case of bending tests, our background research showed that no commercial solution allowing for reliable investigation of so called fracture process zone in quasi-brittle materials is currently available. Thus, this paper is focused on description of recently developed in-situ four-point bending loading device and its instrumentation for testing of quasi-brittle materials. Proof of concept together with the pilot experiments were successfully performed in a CT scanner TORATOM. Based on results of the pilot experiments, we demonstrate that crack development and propagation in a quasi-brittle material can be successfully observed in 3D using high resolution 4D micro-CT under loading.

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