Deformability of Human Mesenchymal Stem Cells Is Dependent on Vimentin Intermediate Filaments

Sharma, Poonam; Bolten, Zachary T.; Wagner, Diane R.; Hsieh, Adam H.

doi:10.1007/s10439-016-1787-z

Cited by 30 publications

(25 citation statements)

References 36 publications

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“…Some studies have demonstrated that the changes of cytoskeleton and adhesion ability influenced the invasive potential and motility of cancer cells . Our results suggested that AmotP130 expression status was related with the arrangements of the cytoskeleton.…”

Section: Resultssupporting

confidence: 55%

“…Some studies have demonstrated that the changes of cytoskeleton and adhesion ability influenced the invasive potential and motility of cancer cells. 26,27 Our results suggested that AmotP130 expression status was related with the arrangements of the cytoskeleton. Therefore, we measured the invasion ability and motility of of the cell at 30 minutes, while the MM231OE cells showed no obvious protrusions until 1.5 hours ( Figure 3E).…”

Section: Amotp130 Affected Cell Motor Ability and Invasion Ability mentioning

confidence: 63%

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AmotP130 regulates RhoGTPase and decreases breast cancer cell mobility

Chen

Jiao

Shen

et al. 2018

J Cellular Molecular Medi

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Angiomotin (Amot) is a newly discovered, multifunctional protein that is involved in cell migration and angiogenesis. However, the role of its isoform, AmotP130, in the regulation of cytoskeleton and metastasis of breast cancer, is unclear. The aim of this study was to investigate the role of AmotP130 in the reorganization of the actin cytoskeleton and the changes of morphology in breast cancer cells through the Rho pathway that influences the invasion and migration of cells. The results suggested that AmotP130 suppressed the invasion ability through remodelling the cytoskeleton of breast cancer cells, including the actin fibre organization and focal adhesion protein turnover. Global transcriptome changes in breast cancer cells following knockdown of AmotP130 identified pathways related with the cytoskeleton and cell motility that involved the Rho GTPase family. From database analyses, changes in the Rho GTPase family of proteins were identified as possible prognostic factors in patients with breast cancer. We have been suggested that AmotP130 suppressed the invasion ability through remodelling of the cytoskeleton of breast cancer cells, involving regulation of the Rho pathway. The cytoskeleton‐related pathway components may provide novel, clinically therapeutic targets for breast cancer treatment.

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Section: Resultssupporting

confidence: 55%

Section: Amotp130 Affected Cell Motor Ability and Invasion Ability mentioning

confidence: 63%

AmotP130 regulates RhoGTPase and decreases breast cancer cell mobility

Chen

Jiao

Shen

et al. 2018

J Cellular Molecular Medi

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“…It is generally considered that vimentin forms passive scaffolding, which mechanically transmits the pulling forces generated by actomyosin but also protects the cells from fast and large mechanically induced deformation . However, actin microfilaments and microtubules can compensate for its action when it is deficient . Vimentin also plays a role in actin‐driven nucleus positioning during migration .…”

Section: Nuclear Structures and Nuclear Cytoskeletal Interactionsmentioning

confidence: 99%

Role of the Nucleus as a Sensor of Cell Environment Topography

Anselme

Wakhloo

Rougerie

et al. 2017

Adv Healthcare Materials

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The proper integration of biophysical cues from the cell vicinity is crucial for cells to maintain homeostasis, cooperate with other cells within the tissues, and properly fulfill their biological function. It is therefore crucial to fully understand how cells integrate these extracellular signals for tissue engineering and regenerative medicine. Topography has emerged as a prominent component of the cellular microenvironment that has pleiotropic effects on cell behavior. This progress report focuses on the recent advances in the understanding of the topography sensing mechanism with a special emphasis on the role of the nucleus. Here, recent techniques developed for monitoring the nuclear mechanics are reviewed and the impact of various topographies and their consequences on nuclear organization, gene regulation, and stem cell fate is summarized. The role of the cell nucleus as a sensor of cell-scale topography is further discussed.

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“…Vimentin is a widely expressed and highly conserved 57-kD protein that constitutes type III intermediate filaments and is constitutively expressed in mesenchymal cells, being important in their development, integrity, and maintenance [93], and modifications in these intermediate filaments can lead to morphological and functional changes [94,95]. Vimentin is o en identified on the surface and intracellular filament of MSCs and different tissues, namely the OM [96] and on olfactory receptor neurons which continue to express this marker unlike other postmitotic neurons that cease vimentin expression as nerve development progresses [97].…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study

Alvites

Branquinho

Amorim

et al. 2020

International Journal of Cell Biology

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Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.

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Deformability of Human Mesenchymal Stem Cells Is Dependent on Vimentin Intermediate Filaments

Abstract: ABSTRACT

Cited by 30 publications

References 36 publications

AmotP130 regulates RhoGTPase and decreases breast cancer cell mobility

AmotP130 regulates RhoGTPase and decreases breast cancer cell mobility

Role of the Nucleus as a Sensor of Cell Environment Topography

Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study

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