Definitive tests for dengue fever: when and which should I use?

Chan, Hui Bin Yvonne; How, Choon How; Ng, Chung Wai Mark

doi:10.11622/smedj.2017100

Cited by 27 publications

(22 citation statements)

References 17 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 Ordering a dengue NS-1 antigen assay within the first week of symptom onset is one of the initial investigations recommended by the Ministry of Health, Singapore, for dengue infections. 35 NS1 test detects at the same time as viral RNA and before an antibody response, so the time to do the best examination is day 0 to day 4 and can be detected before the decrease in platelets. our study included several patients with negative NS-1 results when examined using the Panbio rapid test.…”

Section: Discussionmentioning

confidence: 99%

MCP-1 LEVELS AND ATYPICAL LYMPHOCYTES IN EARLY FEVER OF DENGUE VIRUS INFECTION WITH NON-STRUCTURAL PROTEIN 1 (NS-1) ANTIGEN TEST IN dr DARSONO HOSPITAL, PACITAN

Agustiningrum

Nugraha

Kahar

2020

IJTID

View full text Add to dashboard Cite

Dengue infection caused by DENV and transmitted by mosquitoes Aedes aegypti and Aedes albopictus is a major health problem in the world, including Indonesia. Clinical manifestations of dengue infection are very widely, from asymptomatic until dengue shock syndrome (DSS). DENV will attack macrophages and dendritic cells (DC) and replicate them. Monocytes are macrophages in the blood (± 10% leukocytes). Macrophages produce cytokines and chemokines such as monocyte chemotactic protein-1 (MCP-1)/CCL2. The monocytes that are infected with DENV will express MCP-1, which will increase the permeability of vascular endothelial cells so that they have a risk of developing DHF/DSS. Macrophages and DC secrete NS1 proteins, which are the co-factors that are needed for viral replication and can be detected in the early phase of fever. The increased MCP-1 levels in dengue infection followed by an increase in the number of atypical lymphocytes indicate the arrival of macrophages and monocytes to the site of inflammation which triggers proliferation rather than lymphocytes. This is an observational analytical study with a cross-sectional design to determine the MCP-1 level in dengue infection patients with 1st until the 4th day of fever and the presence of a typical lymphocytes. Dengue infection was determined by rapid tests NS1 positive or negative and MCP-1 levels were measured using by ELISA sandwich method.MCP-1 level of sixty patients dengue infection NS-1 rapid positive or negative with 2nd until 4rt fever were significantly higher than healthy subjects (420.263 ± 158,496 vs 29, 475 ± 23.443;p=0.000), but there was no significant difference in subjects with DF, DHF or DSS (436,47 ± 225,59 vs 422,77 ± 170,55 vs 448,50 ± 117,39; p =0.844). A typically lymphocytes differs significantly in healthy subjects than subjects infected with DENV an average of 2% (p= 0,000). In conclusion, this shows the arrival of macrophages and monocytes to the site of inflammation, which triggers the proliferation of lymphocytes. ABSTRAK Infeksi dengue disebabkan DENV ditularkan oleh nyamuk Aedes aegypti dan Aedes albopictus yang masalah kesehatan utama di dunia, termasuk Indonesia. Manifestasi klinik infeksi dengue sangat bervariasi, dapat asimptomatik sampai dengue shock syndrome (DSS). DENV akan menyerang makrofag juga sel dendritik (DC) dan akan bereplikasi. Monosit merupakan makrofag dalam darah (±10% leukosit). Makrofag memproduksi sitokin dan kemokin seperti MCP 1. MCP-1 terekspresi oleh monosit terinfeksi DENV yang dapat meningkatkan permeabilitas sel endotel vaskular sehingga memiliki risiko mengalami DHF/ DSS. Makrofag dan DC mengeluarkanCorresponding Author.protein NS1 merupakan co-factor yang dibutuhkan untuk replikasi virus dan dapat dideteksi pada fase awal demam.Adanya peningkatan kadar MCP-1 pada infeksi dengue diikuti dengan peningkatan jumlah limfosit biru menunjukkan datangnya makrofag dan monosit ke tempat terjadinya inflamasi yang memicu proliferasi daripada limfosit. Penelitian ini merupakan penelitian observasional analitik ...

show abstract

Section: Discussionmentioning

confidence: 99%

MCP-1 LEVELS AND ATYPICAL LYMPHOCYTES IN EARLY FEVER OF DENGUE VIRUS INFECTION WITH NON-STRUCTURAL PROTEIN 1 (NS-1) ANTIGEN TEST IN dr DARSONO HOSPITAL, PACITAN

Agustiningrum

Nugraha

Kahar

2020

IJTID

View full text Add to dashboard Cite

show abstract

“…The detection of NS1Ag offers reliable point of care diagnosis of acute dengue infection with a sensitivity of 90% in primary infection and 60 to 80% in secondary infection. 9,10 Although specificity is high (97-100%) for IgM antibodies in CSF, it has low sensitivity. Poor sensitivity of IgM antibodies in CSF for dengue virus makes it a less reliable screening tool.…”

Section: Discussionmentioning

confidence: 99%

Dengue Infection: Not Just Fever—A Rare Presentation with Literature Review

Baby

Gilvaz

Santhakumar

et al. 2019

Journal of Pediatric Neurology

View full text Add to dashboard Cite

Dengue fever is a common viral infection in the tropical areas, especially in India. However, dengue infection presenting as hemorrhagic encephalitis is rare. A 10-year-old girl presented with fever, headache, vomiting, and seizures followed by altered sensorium. Her blood serum was positive for antidengue immunoglobulin M enzyme-linked immunosorbent assay (ELISA) and NS1Ag, and cerebrospinal fluid study showed lymphocytic pleocytosis. Magnetic resonance imaging T2-weighted and fluid-attenuated inversion recovery (FLAIR) images showed hyperintensities involving the bilateral thalami, pons, and midbrain with patchy hemorrhage. Despite all supportive measures, she expired 3 days after admission. Our case highlights that dengue encephalitis may present even in the absence of other classical signs.

show abstract

“…Locally available rapid point-of-care tests, which include both assays in one convenient test kit, can enhance dengue diagnosis in an endemic setting." (1) As a matter of fact, dengue fever is presently a public health problem in Southeast Asia and early diagnosis is the main determinant of success in case management. (2) We would hereby like to share our ideas and experience on this issue.…”

Section: E T T E R T O T H E E D I T O Rmentioning

confidence: 99%