Definition of Two New Groups of Atypical Rotaviruses

Pedley, Steve; Bridger, Janice C.; Chasey, D.; McCrae, Malcolm A.

doi:10.1099/0022-1317-67-1-131

Cited by 132 publications

(89 citation statements)

References 31 publications

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“…These were similar to the variations seen among orthoreovirues (Fields, 1982), rotaviruses (Pedley et al, 1986) or orbiviruses (Gorman et al, 1981). The viruses could also be separated on the basis of differences in mol.…”

Section: Discussionsupporting

confidence: 59%

“…The genus Rotavirus is composed of viruses with 11 segments of dsRNA, but electrophoresis of RNA segments from the five currently recognized groups (Pedley et al, 1986) yields segment patterns quite different from the electropherotypes of these viruses of fish and shellfish. Also, the sharp margin of the outer capsid layer makes the Rotavirus virion quite distinct by electron microscopy (Holmes, 1983).…”

Section: Discussionmentioning

confidence: 99%

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Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

Winton

Lannan

Fryer

et al. 1987

Journal of General Virology

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SUMMARYThe morphological, biochemical and growth characteristics of four members of the Reoviridae, three from the fish hosts, golden shiner (Notemigonus crysoleucas), chum salmon (Oncorhynchus keta) and channel catfish (Ictalurus punetatus) and one from American oyster (Crassostrea virginica), were compared. Electron microscopy of negatively stained virions revealed icosahedral particles approximately 75 nm in diameter composed of a double capsid. Complete particles had buoyant densities in CsC1 of 1.34 to 1.36 g/ml. The viruses replicated well in several fish cell lines, forming plaque-like syncytia in monolayer cultures. Each virus could be distinguished by the range of cell lines supporting its growth. Polyacrylamide gel electrophoresis showed that the genome of each virus was composed of 11 segments of dsRNA distributed among three size classes. There were three large, three medium and five small segments in each genome and each isolate had a unique electropherotype. The segments ranged from 2.5 × 10 6 to 0"31 X 106 mol. wt. with a total genome of approximately 15 × 10 6 mol. wt. Analysis by SDS-PAGE revealed that each virus had five major structural proteins. There were two large polypeptides of approximately 135 000 and 125 000 mol. wt., one medium size polypeptide of 70000 mol. wt. and two small polypeptides of 45000 and 34000 mol. wt. Of the major structural proteins, those of approximately 70000 and 34000 tool. wt. were consistently present in the highest concentrations.Minor virion proteins were detected but were not characterized. These four viruses, isolated from aquatic animals, were unlike viruses of the six established genera of the Reoviridae.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

Winton

Lannan

Fryer

et al. 1987

Journal of General Virology

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“…Recently, novel rotavirus strains with limited sequence homologies to group B rotaviruses (strains J19 and B219) have been identified in adults with diarrhea but are without a final classification (31,51,77). Group A, B, and C rotaviruses are found in both humans and animals, whereas group E rotaviruses have been detected only in pigs and group D, F, and G rotaviruses have been detected only in birds (21,58,59,62,66). So far, nucleotide sequences are available only for group A, B, and C rotaviruses.…”

mentioning

confidence: 99%

The Genome Segments of a Group D Rotavirus Possess Group A-Like Conserved Termini but Encode Group-Specific Proteins

Trojnar

Otto

Roth

et al. 2010

J Virol

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Rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. They are grouped according to gene composition and antigenicity of VP6. Whereas group A, B, and C rotaviruses are found in humans and animals, group D rotaviruses have been exclusively detected in birds. Despite their broad distribution among chickens, no nucleotide sequence data exist so far. Here, the first complete genome sequence of a group D rotavirus (strain 05V0049) is presented, which was amplified using sequence-independent amplification strategies and degenerate primers. Open reading frames encoding homologues of rotavirus proteins VP1 to VP4, VP6, VP7, and NSP1 to NSP5 were identified. Amino acid sequence identities between the group D rotavirus and the group A, B, and C rotaviruses varied between 12.3% and 51.7%, 11.0% and 23.1%, and 9.5% and 46.9%, respectively. Segment 10 of the group D rotavirus has an additional open reading frame. Generally, phylogenetic analysis indicated a common evolution of group A, C, and D rotaviruses, separate from that of group B. However, the NSP4 sequence of group C has only very low identities in comparison with cogent sequences of all other groups. The avian group A NSP1 sequences are more closely related to those of group D than those of mammalian group A rotaviruses. Most interestingly, the nucleotide sequences at the termini of the 11 genome segments are identical between group D and group A rotaviruses. Further investigations should clarify whether these conserved structures allow an exchange of genome segments between group A and group D rotaviruses.Rotaviruses are a major cause of acute gastroenteritis in young children (22,32,54,55). They are also etiological agents of diarrhea in several mammalian and avian species (7,9,10,39,52,65,75). Rotaviruses belong to the family Reoviridae and have a nonenveloped viral capsid containing a genome of 11 double-stranded RNA (dsRNA) segments (21, 62). The outer layer of the virus particle is formed by VP4 and VP7 proteins, which possess neutralization antigens. The intermediate layer consists of VP6, a conserved protein, which defines the rotavirus groups. The inner layer is formed by VP2 surrounding a complex of VP1 and VP3, which represent the RNA-dependent RNA polymerase and the guanylyl transferase, respectively (12, 60), and the viral genomic RNAs. In addition, at least five nonstructural proteins are encoded by the rotavirus genome and have diverse functions, e.g

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“…VP6 carries the group and subgroup determinants of rotaviruses and five groups (A to E) are currently defined (Pedley et al, 1986). The presence or absence of particular epitopes on VP6, as determined by reactivity with subgroup-specific monoclonal antibodies (MAbs), allows Group A rotaviruses, the major cause of acute gastroenteritis in children worldwide, to be further…”

mentioning

confidence: 99%

Sequences of VP6 genes of human rotavirus strain RV3 and its vaccine derivative

Palombo

Bishop²

1994

Journal of General Virology

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The nucleotide sequence of the genomic segment encoding the major antigen, VP6, of human rotavirus strain RV3 was determined for the virus isolated from faeces. This was compared with the sequence of the cognate gene of tissue culture-adapted candidate vaccine virus that was derived from RV3 and had been passaged 30 times. A single silent nucleotide difference was detected between the two genes and the deduced amino acid sequence for both showed the highest degree of identity (~> 97"5 %) with the VP6 proteins of rotavirus strains belonging to the same subgroup. The amino acid residues that might affect VP6 subgroup epitope type from RV3 and other viruses of the same or different subgroups were compared. The commonality of particular amino acids among strains of different subgroups suggests that the presence of all subgroup-specific amino acids may be necessary for subgroup determination.

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Definition of Two New Groups of Atypical Rotaviruses

Cited by 132 publications

References 31 publications

Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

Morphological and Biochemical Properties of Four Members of a Novel Group of Reoviruses Isolated from Aquatic Animals

The Genome Segments of a Group D Rotavirus Possess Group A-Like Conserved Termini but Encode Group-Specific Proteins

Sequences of VP6 genes of human rotavirus strain RV3 and its vaccine derivative

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