Definition of chromosome aberrations in testicular germ cell tumor cell lines by 24-color karyotyping and complementary molecular cytogenetic analyses

Summersgill, Brenda; Jafer, Osman; Wang, Rubin; Göker, Hakan; Niculescu-Duvaz, Ioana; Huddart, Robert; Shipley, Janet

doi:10.1016/s0165-4608(01)00414-9

Cited by 24 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was supported by our interphase FISH analysis ( Figure 1) and is consistent with previous interphase FISH studies (Roelofs et al, 2000). A small increase in copy number of markers from the amplified region has also been noted in a cell line (Summersgill et al, 2001b). Subtle differences in the copy number of markers can make it difficult to delineate the extent of amplification by these methods.…”

Section: Discussionsupporting

confidence: 91%

“…This was carried out as previously described using the QUIPS CGH analysis software (Vysis, Chicago, IL, USA) (Summersgill et al, 1998b). A further four cases with previously published amplicons at 12p11.2-p12.1 were available (Summersgill et al, 2001b;Zafarana et al, 2002). The primary samples and cell lines indicated in Table 1 were used for the CESH and microarray analyses.…”

Section: Sample Selection and Preparationmentioning

confidence: 99%

See 1 more Smart Citation

Expression profile of genes from 12p in testicular germ cell tumors of adolescents and adults associated with i(12p) and amplification at 12p11.2–p12.1

et al. 2003

View full text Add to dashboard Cite

Gain of 12p material is invariably associated with testicular germ cell tumors (TGCTs) of adolescents and adults, most usually as an isochromosome 12p. We analyzed TGCTs with i(12p) using a global approach to expression profiling targeting chromosomes (comparative expressed sequence hybridization, CESH). This indicated overexpression of genes from 12p11.2-p12.1 relative to testis tissue and fibroblasts. The nonseminoma subtype showed higher levels of expression than seminomas. Notably, 12p11.2-p12.1 is amplified in about 10% of TGCTs and CESH analysis of such amplicon cases showed high levels of overexpression from this region. Microarray analysis, including cDNA clones representing most UniGene clusters from 12p11.2-p12.1, was applied to DNA and RNA from 5 TGCTs with amplification of 12p11.2-p12.1 and seven TGCTs with gain of the entire short arm of chromosome 12. Expression profiles were consistent with the CESH data and overexpression of EST595078, MRPS35 and LDHB at 12p11.2-p12.1 was detected in most TGCTs. High-level overexpression of BCAT1 was specific to nonseminomas and overexpression of genes such as CMAS, EKI1, KRAS2, SURB7 and various ESTs correlated with their amplification. Genes such as CCND2, GLU3, LRP6 and HPH1 at 12p13 were also overexpressed. The overexpressed sequences identified, particularly those in the region amplified, represent candidate genes for involvement in TGCT development.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Sample Selection and Preparationmentioning

confidence: 99%

Expression profile of genes from 12p in testicular germ cell tumors of adolescents and adults associated with i(12p) and amplification at 12p11.2–p12.1

et al. 2003

View full text Add to dashboard Cite

show abstract

“…These were GCT27, GCT44, Tera1, Tera2, H12.2, 2102Ep, 577MF, NTERA2, SUSA and TCam-2. The cell lines were cultured as described previously (Pera et al, 1987;Kelland et al, 1992;Mizuno et al, 1993;Summersgill et al, 2001;Goddard et al, 2007). A pool of 45 individual samples of normal testis RNA was available from Clontech (Mountain View, CA, USA) and normal DNA from Sigma (St Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Genomic copy number and expression patterns in testicular germ cell tumours

McIntyre

Summersgill

et al. 2007

Br J Cancer

View full text Add to dashboard Cite

Testicular germ cell tumours of adults and adolescents (TGCT) include seminomas (SE) and nonseminomas (NS), with spermatocytic seminomas (SSE) representing a distinct entity in older men. SE and NS have gain of 12p material in all cases, whereas SSE are associated with overrepresentation of chromosome 9. Here, we compare at the chromosomal level, copy number imbalances with global expression changes, identified by comparative expressed sequence hybridisation analyses, in seven SE, one combined tumour, seven NS and seven cell lines. Positive correlations were found consistent with copy number as a main driver of expression change, despite reported differences in methylation status in SE and NS. Analysis of chromosomal copy number and expression data could not distinguish between SE and NS, in-keeping with a similar genetic pathogenesis. However, increased expression from 4q22, 5q23.2 and 9p21 distinguished SSE from SE and NS and decreased copy number and expression from 2q36 -q37 and 6q24 was a specific feature of NS-derived cell lines. Our analysis also highlights 19 regions with both copy number and expression imbalances in greater than 40% of cases. Mining available expression array data identified genes from these regions as candidates for involvement in TGCT development. Supplementary data is available at

show abstract

“…Ethical approval and appropriate consent has been obtained for the use of samples in this study. The cell lines Tera1, Tera2, GCT27, and GCT44 were cultured as previously described (10). Normal diploid DNA (Promega, Leiden, the Netherlands) and RNA from normal pooled testis tissues from 45 samples (BD Biosciences, Palo Alto, CA) were used for reference.…”

Section: Methodsmentioning

confidence: 99%

Amplification and Overexpression of the KIT Gene Is Associated with Progression in the Seminoma Subtype of Testicular Germ Cell Tumors of Adolescents and Adults

et al. 2005

Self Cite

View full text Add to dashboard Cite

We have previously identified amplification at 4q12 in testicular germ cell tumors of adolescents and adults centered around the KIT gene encoding a tyrosine kinase transmembrane receptor. Analysis of primary testicular germ cell tumors totaling 190 cases revealed 21% of the seminoma subtype with an increased copy number of KIT whereas this change was rarely found in the nonseminomas. In most cases, gain of KIT did not include the immediately flanking noncoding DNA or the flanking genes KDR and PDGFRA. Increased copy number of KIT was not found in the putative precursor lesion, carcinoma in situ (CIS), adjacent to tumor with this change. KIT overexpression was found independent of gain and KIT immunostaining was stronger in selected cases with gain of KIT compared to those without. Taken together with activating mutations of KIT in exon 17 identified in 13% of seminomas, this suggests that the KIT gene product plays a role in the progression of CIS towards seminoma, the further understanding of which may lead to novel less toxic therapeutic approaches. (Cancer Res 2005; 65(18): 8085-89)

show abstract

Definition of chromosome aberrations in testicular germ cell tumor cell lines by 24-color karyotyping and complementary molecular cytogenetic analyses

Cited by 24 publications

References 33 publications

Expression profile of genes from 12p in testicular germ cell tumors of adolescents and adults associated with i(12p) and amplification at 12p11.2–p12.1

Expression profile of genes from 12p in testicular germ cell tumors of adolescents and adults associated with i(12p) and amplification at 12p11.2–p12.1

Genomic copy number and expression patterns in testicular germ cell tumours

Amplification and Overexpression of the KIT Gene Is Associated with Progression in the Seminoma Subtype of Testicular Germ Cell Tumors of Adolescents and Adults

Contact Info

Product

Resources

About