Defining Theoretical Limits of Aberration-Corrected Electron Tomography: New Bounds for Resolution, Object Size, and Dose

Achieving High-resolution of Large Specimens Using Aberration-corrected Tomography

“…In aberration-corrected tomography each through-focal stack is mapped to Fourier (k-) space to fill information within a 3D contrast transfer function (CTF) [4]. The object is reconstructed from a simple inverse Fourier transform.…”

“…Remarkably, aberration-corrected scanning transmission electron tomography can offer dose-efficient 3D reconstruction that measures complete specimen information of unbounded object sizes up to a specified cutoff resolution [4]. With aberration-corrected electron tomography, defocus and specimen tilt are combined (Figure 1a,b).…”

Filling in the Missing Wedge with Aberration-corrected Electron Tomography

“…Without any prior assumptions, the small missing wedges of information can be measured using aberration-corrected STEM tomography that acquires a through-focal stack at every tilt [7]. Using a 3D linear incoherent imaging model, information is no longer mapped to a plane in Fourier (k) space, but becomes a volumetric toroid [8]. Figure shows the 3D structure of a contrast transfer function (CTF) for through focal tomography where every specimen tilt measures a toroid with petal-shaped cross-section.…”