Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus

Howson, Emma L.A.; Kurosaki, Yohei; Yasuda, Jiro; Takahashi, Masayoshi; Goto, Hiroaki; Gray, Ashley R.; Mioulet, Valérie; King, Donald P.; Fowler, Veronica L.

doi:10.1016/j.jviromet.2017.08.013

Cited by 34 publications

(39 citation statements)

References 24 publications

(64 reference statements)

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“…With further field validation, the results from two3 could potentially be accepted with the same level of confidence as the reference lab‐based platforms, especially for oral and nasal swabs and specifically vesicular flu acquired during the acute phase of infection. Similar results have been reported for FMDV on other field‐deployable real‐time molecular assays/platforms (Howson et al, ; Howson, Armson, et al, ; Howson, Kurosaki, et al, ; Moniwa et al, ). The fact that two3 detected FMDV in the absence of RNA extraction further potentiates its utility for onsite testing.…”

Section: Resultssupporting

confidence: 86%

“…Some of the field‐deployable molecular assays for FMD have been proven field ready (Howson et al, ; Howson, Armson, et al, ; Howson, Kurosaki, et al, ). However, two3 being a handheld, smart phone‐operated device, it is very easy to transport and does both real‐time PCR and isothermal molecular detection of nucleic acids with run programming that is customizable by the end‐user, providing results within 30–60 min.…”

Section: Resultsmentioning

confidence: 99%

“…Real‐time reverse transcription polymerase chain reaction (RRT‐PCR) assays for FMDV are highly sensitive (Callahan et al, ; Howson, Armson, et al, ; Moniwa, Clavijo, Li, Collignon, & Kitching, ; Rasmussen, Uttenthal, Stricker, Belak, & Storgaard, ) but are mostly restricted to specialized laboratories. Nevertheless, significant progress has been made on field‐deployable molecular assays for FMDV (Ambagala et al, ; Dukes, King, & Alexandersen, ; Howson et al, ; Howson, Armson, et al, ; Howson, Kurosaki, et al, ; Kasanga et al, ; Waters et al, ). However, a sensitive handheld platform has not been described for FMDV.…”

Section: Introductionmentioning

confidence: 99%

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Foot‐and‐mouth disease virus detection on a handheld real‐time polymerase chain reaction platform

Hole

Nfon

2019

Transbound Emerg Dis

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Foot-and-mouth disease (FMD) is a highly contagious disease of livestock that requires rapid control. Early detection is critical but transportation of samples to laboratory delays testing. Sensitive and specific field-deployable assays are therefore desirable. Real-time reverse transcription polymerase chain reaction (RRT-PCR) andRRT-loop-mediated isothermal amplification assays for FMDV on portable platforms have been described but none of these are handheld. In this report, we have evaluated a handheld Biomeme two3™ Real-Time PCR Thermocycler (two3) as a fielddeployable platform for FMDV RRT-PCR targeting the 3D gene segment. Two3's performance was compared with the laboratory-based reference assay on the ABI7500 platform. RNA extraction using a rapid Biomeme proprietary sample prep technology (M1) was compared with MagMax RNA extraction. Two3 successfully detected FMDV isolates for six serotypes (O, A, Asia 1, SAT 1, 2 and 3). Serotype C was excluded since it has not been detected in the field since 2004. The limits of detection for serial 10-fold dilutions of cell culture isolates were equal or one log different between two3 and ABI7500. Furthermore, two3 detected FMDV RNA in multiple sample types including serum, vesicular fluid, tissue suspensions, oral fluid, oral and nasal swabs. Two3 also detected FMDV RNA directly in vesicular fluid and other samples without prior RNA extraction. Comparison of the time to first detection of a positive result in serial samples in MagMax RNA extraction/ABI7500 (MgMx/ABI) system vs. M1 RNA extraction/Two3 system revealed similar or slightly better analytical sensitivity for the MgMx/ABI system. Overall, RNA extraction by M1 yielded good results and FMDV RNA detection on two3 was not significantly different from the ABI7500. Therefore, two3 could potentially enable sensitive penside detection of FMDV within an hour using M1-extracted RNA or direct testing of vesicular fluid and swabs without RNA extraction thereby ensuring prompt implementation of appropriate control measures. K E Y W O R D S Foot-and-mouth disease virus, handheld, polymerase chain reaction Reproduced with the permission of the Minister of Health.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Foot‐and‐mouth disease virus detection on a handheld real‐time polymerase chain reaction platform

Hole

Nfon

2019

Transbound Emerg Dis

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“…S7). RT-RPA also exhibits nonspecific amplification [66][67][68] , and therefore, alternative isothermal amplification technologies, such as RT-LAMP 41,69 , could be implemented to improve specificity. Although RT-LAMP is likely to improve specificity as well as sensitivity, the requirement of two separate reactions remains problematic, increasing the likelihood of contamination due to sample transfers 70 .…”

Section: Discussionmentioning

confidence: 99%

A Sensitive, Rapid, and Portable CasRx-based Diagnostic Assay for SARS-CoV-2

Brogan

Chaverra-Rodríguez

Lin

et al. 2020

Preprint

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Since its first emergence from China in late 2019, the SARS-CoV-2 virus has spread globally despite unprecedented containment efforts, resulting in a catastrophic worldwide pandemic. Successful identification and isolation of infected individuals can drastically curtail virus spread and limit outbreaks. However, during the early stages of global transmission, point-of-care diagnostics were largely unavailable and continue to remain difficult to procure, greatly inhibiting public health efforts to mitigate spread. Furthermore, the most prevalent testing kits rely on reagent- and time-intensive protocols to detect viral RNA, preventing rapid and cost-effective diagnosis. Therefore the development of an extensive toolkit for point-of-care diagnostics that is expeditiously adaptable to new emerging pathogens is of critical public health importance. Recently, a number of novel CRISPR-based diagnostics have been developed to detect COVID-19. Herein, we outline the development of a CRISPR-based nucleic acid molecular diagnostic utilizing a Cas13d ribonuclease derived from Ruminococcus flavefaciens (CasRx) to detect SARS-CoV-2, an approach we term SENSR (Sensitive Enzymatic Nucleic-acid Sequence Reporter). We demonstrate SENSR robustly detects SARS-CoV-2 sequences in both synthetic and patient-derived samples by lateral flow and fluorescence, thus expanding the available point-of-care diagnostics to combat current and future pandemics.

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“…As described earlier, as an isothermal DNA amplification method, RPA has been widely used in the detection of different pathogens. The newly established FMDV RT-RPA-LFD assay has a higher sensitivity, up to 10 copies as compared with the previous FMDV RT-RPA assays (14,40) with sensitivity limited to 100 RNA copies. Furthermore, this RT-RPA-LFD assay only requires a thermos metal bath at 38 • C unlike other previous RT-RPA assays, which need a sophisticated instrumentation, and the RPA amplicons can be detected by LFD within 5 min.…”

Section: Chromatographic Strip Testsmentioning

confidence: 95%

Advances in the Diagnosis of Foot-and-Mouth Disease

et al. 2020

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Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically.

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Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus

Cited by 34 publications

References 24 publications

Foot‐and‐mouth disease virus detection on a handheld real‐time polymerase chain reaction platform

Foot‐and‐mouth disease virus detection on a handheld real‐time polymerase chain reaction platform

A Sensitive, Rapid, and Portable CasRx-based Diagnostic Assay for SARS-CoV-2

Advances in the Diagnosis of Foot-and-Mouth Disease

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