“…The PCR amplification was performed as previously described (Hu, Yang, Long, Liu, & Rengel, 2016). The PCR products were confirmed, purified, and quantified as previously described (Huang, Yang, & Li, 2016). The quality and quantity of the amplicon library were assessed on a Bioanalyzer (Agilent, Santa Clara, CA, U.S.A.) and with the Library Quantification Kit for Illumina (Kapa Biosciences, Wilmington, MA, U.S.A.), respectively.…”