2015
DOI: 10.1128/ec.00267-14
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Defining the Morphology and Mechanism of the Hemoglobin Transport Pathway in Plasmodium falciparum-Infected Erythrocytes

Abstract: Hemoglobin degradation during the asexual cycle of Plasmodium falciparum is an obligate process for parasite development and survival. It is established that hemoglobin is transported from the host erythrocyte to the parasite digestive vacuole (DV), but this biological process is not well characterized. Three-dimensional reconstructions made from serial thin-section electron micrographs of untreated, trophozoite-stage P. falciparum-infected erythrocytes (IRBC) or IRBC treated with different pharmacological age… Show more

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Cited by 70 publications
(89 citation statements)
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“…Dynamins are guanine triphosphatases (GTPases) best known for their role in fission of endocytic vesicles forming at plasma membrane into the cytosol, but can also promote membrane fission at the TGN, endosomes and mitochondria T. gondii DrpB localizes to the TGN and potentially the ELCs, but has not been observed at the parasite plasma membrane . However, given that dynamin‐dependent fission of endocytic vesicles at the plasma membrane is conserved in plants, mammals, fungi and other protozoan parasites including Plasmodium, transient populations of DrpB that are difficult to detect by traditional microscopy could promote endocytosis at the plasma membrane similar to the yeast dynamin‐like protein Vps1 . If this is true, then interfering with DrpB function will inhibit ingestion and reduce the percentage of mCherry + parasites.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Dynamins are guanine triphosphatases (GTPases) best known for their role in fission of endocytic vesicles forming at plasma membrane into the cytosol, but can also promote membrane fission at the TGN, endosomes and mitochondria T. gondii DrpB localizes to the TGN and potentially the ELCs, but has not been observed at the parasite plasma membrane . However, given that dynamin‐dependent fission of endocytic vesicles at the plasma membrane is conserved in plants, mammals, fungi and other protozoan parasites including Plasmodium, transient populations of DrpB that are difficult to detect by traditional microscopy could promote endocytosis at the plasma membrane similar to the yeast dynamin‐like protein Vps1 . If this is true, then interfering with DrpB function will inhibit ingestion and reduce the percentage of mCherry + parasites.…”
Section: Resultsmentioning
confidence: 99%
“…given that dynamin-dependent fission of endocytic vesicles at the plasma membrane is conserved in plants, mammals, fungi and other protozoan parasites including Plasmodium,[43][44][45][46][47] transient populations of DrpB that are difficult to detect by traditional microscopy could promote endocytosis at the plasma membrane similar to the yeast dynamin-like protein Vps1. 46 If this is true, then interfering with DrpB function will inhibit ingestion and reduce the percentage of mCherry + parasites.…”
mentioning
confidence: 99%
“…Analogous trafficking and expression processes seem likely in Plasmodium- infected RBCs; however, DNA/membrane interactions and plasmid incorporation happen spontaneously in these cells without any need for electroporation. Plasmid DNA may be taken up with host RBC contents into parasite cytostomes and phagotrophs, which have been found to convey hemoglobin either directly or via intermediate vesicles to a central digestive vacuole [31] [32]. For expression to happen, DNA in these hemoglobin-containing compartments would have to escape for redirection and entry into the nucleus.…”
Section: Discussionmentioning
confidence: 99%
“…Previous ultrastructural studies of the feeding mechanism of P. falciparum employed thin-section electron micrographs [36][37] . There is a need for alternative methods to examine the endocytic process of live and intact cells under less-disruptive conditions [38][39] .…”
Section: Fig 3: (A) Efficiency Of Invasion Of Resealed Erythrocytes mentioning
confidence: 99%