Defining the morphologic features and products of cell disassembly during apoptosis

Tixeira, Rochelle; Caruso, Sarah; Paone, Stephanie; Baxter, Amy A.; Atkin-Smith, Georgia K.; Hulett, Mark D.; Poon, Ivan K. H.

doi:10.1007/s10495-017-1345-7

Cited by 56 publications

(53 citation statements)

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“…This plasma membrane blebbing could be due to the apoptotic process onset. In fact, this process takes place according to well-defined stages that are morphologically recognizable: (i) initially, the cell cytoskeleton breaks up and causes the membrane to bulge outward; (ii) during later stages, these bulges separate from the cell as apoptotic bodies, by carrying with them a portion of cytoplasm, larger organelle fragments, such as larger parts of the fragmented apoptotic cell nucleus [46][47][48]. It has been recently shown that blebs produced by actin-myosin contraction during apoptosis release damage-associated molecular pattern proteins before secondary necrosis onset [47].…”

Section: Incidence On Intercellular Communicationsmentioning

confidence: 99%

Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

Vergallo

Dini

2018

Sustainability

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Abstract:Moderate static magnetic fields (SMFs) are generated from sources such as new-generation electric trams and trains, electric arc welding, and magnetic resonance imaging (MRI) devices, as well as during the industrial production of aluminium, while extremely low frequency pulsed magnetic fields (ELF-PMFs) are produced by house power installations, household appliances, and high voltages transmission lines. Moderate SMFs and ELF-PMFs with magnetic flux densities (B) in the range of 1-60 mT and frequencies (f ) up to 50 Hz are common MF exposure sources for the population. Even though humans are continually exposed to these MFs, to date no definitive endpoint has been drawn about their safety. In this review, the state of knowledge about the biological effects induced by these MFs on different cell types will be addressed. In our own observation, the putative modulation of Ca 2+ /H + and Na + /H + plasma membrane antiporters of human peripheral blood lymphocytes (PBLs) was found to occur after a 24 h exposure to a 6 mT SMF, and the bystander effect observed on U937 cells cultivated for up to 6 h in the conditioned medium harvested from human PBLs previously exposed for 24 h to the same MF (secondary necrosis induction) will be also herein discussed.

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Section: Incidence On Intercellular Communicationsmentioning

confidence: 99%

Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

Vergallo

Dini

2018

Sustainability

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show abstract

“…[2] [5] An apoptotic cell can undergo a series of well-defined morphological steps (as described in detail below) to facilitate the fragmentation of the cell, leading to the formation of membrane-bound vesicles called apoptotic bodies [1][2][5] [6] ( Figure 1). The process of generating apoptotic bodies during apoptosis is known as apoptotic cell disassembly.…”

Section: Introductionmentioning

confidence: 99%

“…[5] [6] The functional significance of this process is still not well understood. Nevertheless, the formation of apoptotic bodies has been hypothesised to mediate intercellular communication and promote efficient clearance of apoptotic cells (a process known as efferocytosis).…”

Section: Introductionmentioning

confidence: 99%

“…[5] [6] The formation of large dynamic membrane blebs could facilitate the fragmentation of organelles such as the nucleus during the progression of apoptosis.…”

Section: Introductionmentioning

confidence: 99%

“…[5] [6] Although the mechanism underpinning the final fragmentation process is not well defined, the disassociation of apoptotic bodies from different types of apoptotic membrane protrusion may require shear stress or perhaps even interaction with neighbouring cells. [5] It should be noted that in addition to apoptotic bodies, membrane-bound vesicles that are smaller than 1 micron in diameter are also released during apoptosis.…”

Section: Introductionmentioning

confidence: 99%

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Cell disassembly during apoptosis

Smith¹,

Parkes²,

Atkin-Smith³

et al. 2017

Wiki J Med

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Frontline Science: Dynamic cellular and subcellular features of migrating leukocytes revealed by in vivo lattice lightsheet microscopy

Manley

Potter

Heddleston

et al. 2020

Journal of Leukocyte Biology

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Neutrophil and macrophage (M) migration underpin the inflammatory response. However, the fast velocity, multidirectional instantaneous movement, and plastic, ever-changing shape of phagocytes confound high-resolution intravital imaging. Lattice lightsheet microscopy (LLSM) captures highly dynamic cell morphology at exceptional spatiotemporal resolution. We demonstrate the first extensive application of LLSM to leukocytes in vivo, utilizing optically transparent zebrafish, leukocyte-specific reporter lines that highlighted subcellular structure, and a wounding assay for leukocyte migration. LLSM revealed details of migrating leukocyte morphology, and permitted intricate, volumetric interrogation of highly dynamic activities within their native physiological setting. Very thin, recurrent uropod extensions must now be considered a characteristic feature of migrating neutrophils. LLSM resolved trailing uropod extensions, demonstrating their surprising length, and permitting quantitative assessment of cytoskeletal contributions to their evanescent form. Imaging leukocytes in blood vessel microenvironments at LLSM's spatiotemporal resolution displayed blood-flow-induced neutrophil dynamics and demonstrated unexpected leukocyte-endothelial interactions such as leukocyte-induced endothelial deformation against the intravascular pressure. LLSM of phagocytosis and cell death provided subcellular insights and uncovered novel behaviors. Collectively, we provide high-resolution LLSM examples of leukocyte structures (filopodia lamellipodia, uropod extensions, vesicles), and activities (interstitial and intravascular migration, leukocyte rolling, phagocytosis, cell death, and cytoplasmic ballooning). Application of LLSM to intravital leukocyte imaging sets the stage for transformative studies into the cellular and subcellular complexities of phagocyte biology.

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Defining the morphologic features and products of cell disassembly during apoptosis

Cited by 56 publications

References 6 publications

Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

Cell disassembly during apoptosis

Frontline Science: Dynamic cellular and subcellular features of migrating leukocytes revealed by in vivo lattice lightsheet microscopy

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