Defining the function of SUMO system in pod development and abiotic stresses in Peanut

Abstract

Background: Posttranslational modification of proteins by small ubiquitin like modifier (SUMO) proteins play an important role during the developmental process and in response to abiotic stresses in plants. However, little is known about SUMOylation in peanut (Arachis hypogaea L.), one of the world's major food legume crops. In this study, we characterized the SUMOylation system from the diploid progenitor genomes of peanut, Arachis duranensis (AA) and Arachis ipaensis (BB). Results: Genome-wide analysis revealed the presence of 40 SUMO system genes in A. duranensis and A. ipaensis. Our results showed that peanut also encodes a novel class II isotype of the SCE1, which was previously reported to be uniquely present in cereals. RNA-seq data showed that the core components of the SUMOylation cascade SUMO1/2 and SCE1 genes exhibited pod-specific expression patterns, implying coordinated regulation during pod development. Furthermore, both transcripts and conjugate profiles revealed that SUMOylation has significant roles during the pod development. Moreover, dynamic changes in the SUMO conjugates were observed in response to abiotic stresses. Conclusions: The identification and organization of peanut SUMO system revealed SUMOylation has important roles during stress defense and pod development. The present study will serve as a resource for providing new strategies to enhance agronomic yield and reveal the mechanism of peanut pod development.

Comparative Repeat Profiling of Two Closely Related Conifers (Larix decidua and Larix kaempferi) Reveals High Genome Similarity With Only Few Fast-Evolving Satellite DNAs

“…We have applied short read sequencing followed by read clustering to efficiently gain insights into both genomes' satDNA contents (as laid out by Weiss-Schneeweiss et al, 2015;Novák et al, 2017). This approach has been successfully used to characterize the repeat landscapes of many non-model plant species as for example beans, various grasses, camellias, crocuses, quinoa, and ferns (Cai et al, 2014;Heitkam et al, 2015;Ávila Robledillo et al, 2018;Kirov et al, 2018;Liu et al, 2019;Schmidt et al, 2019;Heitkam et al, 2020;Ribeiro et al, 2020), and also of non-model animals, such as locusts, grasshoppers, or fishes (Ruiz-Ruano et al, 2016;Ferretti et al, 2020;Boštjančić et al, 2021). For larch genomes, we provided evidence that LTR retrotransposons and derived fragments are their main components, well in line with reports for the related pines and spruces (Kamm et al, 1996;Kossack and Kinlaw, 1999;Nystedt et al, 2013;Stevens et al, 2016;Voronova et al, 2017;Perera et al, 2018).…”

The Major Intrinsic Protein Family and Their Function Under Salt-Stress in Peanut

“…The gene expression in various tissues were investigated by quantitative-polymerase chain reaction (qPCR), and the samples were prepared as previously described (Clevenger et al, 2016). The tissues for gene expression analysis were collected, including radicles, shoots and roots from 20-day-old seedlings, flowers, and aerial and subterranean pegs and fruits in five stages, as described elsewhere (Liu et al, 2019).…”

Plasma Exosome-Derived Sentrin SUMO-Specific Protease 1: A Prognostic Biomarker in Patients With Osteosarcoma

“…Small ubiquitin-related modifier (SUMO) is a small ubiquitin-like protein with a molecular weight of 12 kDa and consisted of about 100 amino acids ( 8 ). The modification of SUMO is mainly catalyzed by SUMO-specific protease (SENP), conjugating enzyme E2 (Ubc9) and E3 ligase ( 9 ). SUMO modification participates in the basic physiological activities of cells, which is one of the necessary mechanisms for growth and development.…”