2013
DOI: 10.1089/scd.2013.0040
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Defining the Diversity of Phenotypic Respecification Using Multiple Cell Lines and Reprogramming Regimens

Abstract: To better understand the basis of variation in cellular reprogramming, we performed experiments with two primary objectives: first, to determine the degree of difference, if any, in reprogramming efficiency among cells lines of a similar type after accounting for technical variables, and second, to compare the efficiency of conversion of multiple similar cell lines to two separate reprogramming regimens-induced neurons and induced skeletal muscle. Using two reprogramming regimens, it could be determined whethe… Show more

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Cited by 6 publications
(10 citation statements)
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“…Parallel analysis of selected mouse lines (Alicea et al 2013; see Supplemental Figure 3, panels C and D) showed a result similar to the human lines, with the exception of was because several independent laboratories had confirmed the production of iN cells using similar methodologies (Vierbuchen et al, 2010;Pfistetrer, et al, 2011;Pang et al, 2011;Caiazzo et al, 2011 (Vierbuchen et al, 2010;Caiazzo et al, 2011) of human origin fused to YFP as shown schematically in Alicea et al 2013 (Supplemental Figure 4). As predicted, all of the YFP-fusion proteins localized primarily to the nucleus, some presenting a mottled appearance and others appearing more diffuse (Figure 2.1, panel A).…”
Section: Resultsmentioning
confidence: 72%
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“…Parallel analysis of selected mouse lines (Alicea et al 2013; see Supplemental Figure 3, panels C and D) showed a result similar to the human lines, with the exception of was because several independent laboratories had confirmed the production of iN cells using similar methodologies (Vierbuchen et al, 2010;Pfistetrer, et al, 2011;Pang et al, 2011;Caiazzo et al, 2011 (Vierbuchen et al, 2010;Caiazzo et al, 2011) of human origin fused to YFP as shown schematically in Alicea et al 2013 (Supplemental Figure 4). As predicted, all of the YFP-fusion proteins localized primarily to the nucleus, some presenting a mottled appearance and others appearing more diffuse (Figure 2.1, panel A).…”
Section: Resultsmentioning
confidence: 72%
“…and satellite cells, but are not expressed at significant levels in fibroblasts (Day, Shefer, and Yablonka-Reuveni, 2010;Day et al, 2007;Ellis et al, 2004;D'Amour and Gage, 2003;Vogel et al, 2003;Avilion et al, 2003;Zimmerman et al, 1994). The results revealed that essentially all cells appeared immunopositive for the fibroblast-associated markers vimentin and fibronectin and no cells appeared positive for the stem cell markers nestin and Sox-2 that were readily observed in control cultures of human neural stem cells (Alicea et al 2013; see Supplemental Figure 3, panel A and B).…”
Section: Resultsmentioning
confidence: 92%
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